Antiangiogenic activity of 4-O-methylgallic acid from Canavalia gladiata, a dietary legume

A Novel Ca2+/Calmodulin Antagonist HBC Inhibits Angiogenesis and Down-regulates Hypoxia-inducible Factor

“…Cell Growth and Viability Assay-Cell growth was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and cell viability was assessed using trypan blue staining as described previously (30).…”

“…Chemoinvasion Assay-The invasiveness of endothelial cells was determined in vitro using a Transwell chamber system with polycarbonate filter inserts with a pore size of 8.0 m as described previously (30). Briefly, the lower side of the filter was coated with 10 l of gelatin (1 mg/ml), and the upper side was coated with 10 l of Matrigel (3 mg/ml).…”

“…Capillary Tube Formation Assay-Capillary tube formation of endothelial cells in vitro was assessed as described previously (30). Briefly, HUVECs (1 ϫ 10 5 cells) were inoculated on the surface of the Matrigel, and HBC or TFP was added for 6 -18 h in the presence or absence of VEGF.…”

The structure, occurrence and biological activity of ellagitannins: a general review

“…Ellagitannins exhibit a wide range of biological activity, refl ected in their anti-atherogenic [Kaplan et al 2001, Khateeb et al 2010, antithrombotic [Teng et al 1997, Umar et al 2003, Crescente et al 2009, De Lange et al 2007, Mattiello et al 2009], anti-infl ammatory , Ngoumfo et al 2008 and anti-angiogenic [Jeon et al 2005, Lee and Lee 2005, Oak et al 2006 properties. Studies have confi rmed a correlation between the consumption of foods rich in ellagitannins (walnuts, pomegranates) and improved cardiovascular health [Beretta et al 2009, Larossa et al 2010.…”

Studies on the regulation of Hematopoietic enhancement of Brassica campestris var narinosa., Canavalia gladiata DC semen and their combinational prescription via Jak2/STAT5/GATA1 Pathway in Sca-1+hematopoietic stem cells

“…작두콩(Canavalia gladiata DC. CGD)의 종자(semen)를 꼬 투리가 작두날 모양 이어서 도두(도두) 9) 라고 하여 암 10) , 모든 종기, 만성신장염, 그리고 빈혈 예방에 효과적이라 보고되었다 11) . 또한 이아소플라본(isoflavone) 중에서 제니스틴 (genistin) 이 항암효과와 폐경기여성의 증상 완화가 보고되어 있다 12) .…”