Antiangiogenic and Antioxidant In Vitro Properties of Hydroethanolic Extract from açaí (Euterpe oleracea) Dietary Powder Supplement

Abstract

The Euterpe oleracea fruit (açaí) is a promising source of polyphenols with health-promoting properties. To our knowledge, few studies have focused on the influence of açaí phytochemicals on angiogenesis, with a significant impact on cancer. This study aimed at investigating the phytochemical profile of a purple açaí hydroethanolic extract (AHE) obtained from a commercial dietary powder supplement by high-performance liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry, and evaluate its in vitro effects on distinct angiogenic steps during vessel growth and on oxidative markers in human microvascular endothelial cells (HMEC-1). The phenolic profile of AHE revealed the presence of significant levels of anthocyanins, mainly cyanidin-3-O-rutinoside, and other flavonoids with promising health effects. The in vitro studies demonstrated that AHE exerts antiangiogenic activity with no cytotoxic effect. The AHE was able to decrease HMEC-1 migration and invasion potential, as well as to inhibit the formation of capillary-like structures. Additionally, AHE increased antioxidant defenses by upregulating superoxide dismutase and catalase enzymatic activities, accompanied by a reduction in the production of reactive oxygen species. These data bring new insights into the potential application of angiogenic inhibitors present in AHE on the development of novel therapeutic approaches for angiogenesis-dependent diseases.

Phenolic composition, antiproliferative and antiulcerogenic activities of a polyphenol‐rich purified extract from açai (Euterpe oleracea) fruits

“…C-3-G and C-3-R were shown to be the major anthocyanins, representing approximately 88% of the total quantified phenolic content, of which 40% and 60% were C-3-G and C-3-R, respectively. The PPEA presented twice higher concentration of anthocyanins than the hydroethanolic extracts from six genotypes of ac ßai reported by Torma et al (2017), which obtained total anthocyanin values ranging from 9.15 to 64.83 mg g À1 DE and higher non-anthocyanin content compared with the hydroethanolic extract from ac ßai (1.23 mg g À1 DE) reported by Costa et al (2021). Another study found from freeze-dried ac ßai a total anthocyanin content of 3.19 mg g À1 DE, of which 36.7% was C-3-G, 60.5% was C-3-R and 2.8% were others anthocyanins (Schauss et al, 2006).…”

“…Another study found from freeze‐dried açai a total anthocyanin content of 3.19 mg g −1 DE, of which 36.7% was C‐3‐G, 60.5% was C‐3‐R and 2.8% were others anthocyanins (Schauss et al ., 2006). In agreement, the predominance of C‐3‐R and C‐3‐G were also previously described in commercial and non‐commercial freeze‐dried açai (Dias et al ., 2012, 2013; Xiong et al ., 2020; Costa et al ., 2021).…”

“…The major non‐anthocyanin compounds were orientin, homoorientin, vitexin, isovitexin, rutin, scoparin, taxifolin deoxyhexose or its isomer, catechin and simple phenols such as vanillic acid and protocatechuic acid. They have also been characterised and reported in other studies with açai (Schauss et al ., 2006; Dias et al ., 2013; Costa et al ., 2021). The content of homoorientin, orientin and vitexin in PPEA (5.60 ± 0.08, 3.33 ± 0.06 and 0.55 ± 0.02 mg g −1 DE, respectively) were found to be higher compared with the content previously described in another hydroethanolic extract from açai (0.12, 0.05 and 0.05 mg g −1 DE, respectively) (Costa et al ., 2021).…”

Angioprevention of Urologic Cancers by Plant-Derived Foods

“…The phenolic profile of purple açaí hydroethanolic extract reveals the presence of significant levels of anthocyanins, mainly cyanidin-3-glucoside, and other flavonoids. In vitro studies demonstrate that the mentioned extract exerts antiangiogenic activity with no cytotoxic effect and abrogates HMEC-1 migration and invasion, as well as their ability to form capillary-like structures [339].…”

The Relieving Effects of a Polyherb-Based Dietary Supplement ColonVita on Gastrointestinal Quality of Life Index (GIQLI) in Older Adults with Chronic Gastrointestinal Symptoms Are Influenced by Age and Cardiovascular Disease: A 12-Week Randomized Placebo-Controlled Trial

“…Acai is a phytochemical plant with antioxidant, antiinflammatory, and antigenotoxic effects [32][33][34][35][36]. Dried extract of acai berries improves ethanol-induced ulcer in rats [37].…”