Antiangiogenic effect of 2-benzoyl–phenoxy acetamide in EAT cell is mediated by HIF-1α and down regulation of VEGF of in-vivo

Prabhakar, B.T.; Khanum, Shaukath Ara; Shashikanth, S.; Salimath, Bharathi P.

doi:10.1007/s10637-006-6587-0

Cited by 32 publications

(10 citation statements)

References 33 publications

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“…Inhibition of neovessel formation following the treatment with compounds 8d , 8l , and 8o (10 µM) in fertilized egg CAM was analyzed and changes in the microvessel density were photographed by using Nikon D3200 camera.…”

Section: Biologymentioning

confidence: 99%

“…In continuation of our previous research toward the development of antiangiogenic as well as anticancer drugs , more specifically benzophenone derivatives, [2‐(4‐methoxy‐benzoyl)‐4‐methylphenoxy]‐ N ‐(4‐chloro‐phenyl)‐acetamide (BP‐1, IC 50 : 42.50 µM) was known to inhibit angiogenesis. It prevents the angiogenesis‐dependent disorders like mammary carcinoma and rheumatoid arthritis both in vivo and in vitro , where it down‐regulated the vascular endothelial growth factor (VEGF) gene expression, which is responsible for angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Design, Synthesis, and Anticancer Properties of Novel Benzophenone‐Conjugated Coumarin Analogs

Ranganatha

Zameer

Meghashri³

et al. 2013

Archiv der Pharmazie

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In the current scenario, development of anticancer drugs with specific targets is of prime importance in modern chemical biology. Observing the importance of benzophenone and coumarin nucleus, it would be worthwhile to design and synthesize novel benzophenone derivatives (8a-o) bearing the coumarin nucleus. Further, they were screened for prospective anticancer activities in vitro against the Michigan Cancer Foundation-7 (MCF-7) and Ehrlich's ascites tumor (EAT) cell lines and their biomarkers, followed by in silico studies regarding phosphoinositide 3-kinase (PI3K) and caspase by molecular docking. Benzophenones have been reported as potential drugs targeting tumor angiogenesis; thus, the formation of neovessels in an in vivo model system like CAM, which is angiogenesis dependent, was observed in the presence of compounds 8a-o. The above findings would help in understanding their putative potential as therapeutic agents for cancer patients.

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Section: Biologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design, Synthesis, and Anticancer Properties of Novel Benzophenone‐Conjugated Coumarin Analogs

Ranganatha

Zameer

Meghashri³

et al. 2013

Archiv der Pharmazie

Self Cite

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“…Tumor tissues fixed with 10% neutral-buffered formalin were processed and subjected to histopathological analysis with hematoxylin and eosin (HE) staining for the measurement of MVD using a Lawrence and Mayo Lynx Reg microscope, as previously described (12). …”

Section: Methodsmentioning

confidence: 99%

Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma

Dai

Jiang

et al. 2017

Oncology Letters

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Abstract. Marsdenia tenacissima (MT), a traditional Chinese medicine, has been utilized in the treatment of a variety of malignant conditions for decades, but the underlying mechanism remains unclear. Angiogenesis, new blood vessel formation by nearby endothelial cells (ECs) from pre-existing vessels, plays a key role in cancer growth. In the present study, the effects of MT extract (MTE) on EC proliferation and apoptosis in vitro, and on A20 mouse lymphoma growth and angiogenesis in vivo were investigated. MTE exhibited an anti-proliferative effect on the ECs, with a half maximal inhibitory concentration of 11.91±0.24 µl/ml. Acridine orange/propidium iodide staining indicated that cell apoptosis increased with MTE concentration. Flow cytometry revealed that the EC apoptosis rates induced by 0, 6.25, 12.5 and 25 µl/ml MTE were 4.8, 23.3, 49.8 and 92.3%, respectively. In vivo, the volume and weight of the A20 solid tumors were significantly inhibited following administration of 300 µl MTE per day for 14 days (P<0.05). MTE showed extended survivability and a satisfactory security. Subsequent to treatment with MTE, peritumorous angiogenesis was significantly reduced, with lower microvessel density (P<0.05) was quantified by hemotoxylin and eosin staining. Moreover, serum vascular endothelial growth factor, matrix metalloproteinase (MMP)-2 and MMP-9 expression at the protein level in the MTE-treated group, quantified using an ELISA, was significantly lower than that of the control (P<0.05). In a chick chorioallantoic membrane assay, 12.5 and 25 µl/ml MTE distinctly decreased the level of angiogenesis (P<0.05). In conclusion, MTE exhibited potent anti-lymphoma efficacy in vitro and this may be associated with its effects against tumor angiogenesis. IntroductionAngiogenesis, the process of the formation of new blood vessels from pre-existing ones, plays a key role in the progression of solid tumors and hematological malignancies, such as lymphoma (1). Anti-angiogenesis can inhibit tumor growth and metastasis, and has become an appealing strategy for tumor therapy.Traditional Chinese medicine (TCM) has been used for several thousand years, and has been suggested to aid the prevention and treatment of diseases such as cancer. Over the past 40 years, a number of TCM herbs have become a focus of interest globally; anticancer components have been isolated from medicinal herbs, which have been demonstrated to serve as modifiers of biological responses and as inhibitors of angiogenesis (2).The stem of Marsdenia tenacissima (MT), also known as Marsdeniae tenacissimae (Roxb) Wight et Arn (family Asclepiadaceae), is well known as 'Tong-guan-teng' in TCM. Over the past 30 years, the antitumor effects of MT have aroused attention and passion from researchers. Previous studies have indicated that MT could inhibit proliferation and induce apoptosis in various cancer cell lines, such as HepG2/Dox, SGC 7901, Lewis lung carcinoma, U937, HL60, Jurkat, Raji and RPMI8226 (3-7). Studies in vitro revealed that MT exerts antitumor activity ...

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“…The cells in the ascites model the life span of mice is restricted to 10-12 days. 14,15 For the induction of solid tumor, Cells were taken from the tumor mouse and without dilution along with the ascetic fluid 1x10 6 cells/animal were injected to thigh region of experimental mouse and observed for the solid tumor to final stage. The animal with solid tumor type will survive for 40-60 days.…”

Section: Tumor Inductionmentioning

confidence: 99%

Differential genotypical expression of a NEDD9 in normal and tumor tissues: a possible pharmacological target

Kumar¹,

Krishna

Prabahkar

2018

Int J Basic Clin Pharmacol

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Background: Neural precursor cell expressed developmentally down regulated-9 (NEDD9) is a E, D, D scaffolding metastatic marker protein in multiple cancer types. Generally, the expression occurs during the embryonic development and depletes in adults. Expression of NEDD9 in adults leads to the progression of tumor which is sufficient for the cellular invasion. Elevated behavior of the gene mediates metastatic movement which includes protease dependent neovessel formation, invasion and migration of tumor cells from the site of origin to the distant tissues.Methods: The current study involves the screening and elucidation of differential expression of NEDD9 in normal and tumor subtypes with various tissues of mice by immunohistochemistry.Results: The validating approaches in the study, low expression of NEDD9 was observed in the normal tissues and predominance in the tumor subsets.Conclusions: The experimental analysis proven that NEDD9 expression is merely associated with tumor progression and the molecular mechanism of NEDD9 is restricted in the establishment of metastatic cascade. NEDD9 association in tumor prognosis which helps in the emergence of diagnostic and therapeutic approaches.

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Antiangiogenic effect of 2-benzoyl–phenoxy acetamide in EAT cell is mediated by HIF-1α and down regulation of VEGF of in-vivo

Cited by 32 publications

References 33 publications

Design, Synthesis, and Anticancer Properties of Novel Benzophenone‐Conjugated Coumarin Analogs

Design, Synthesis, and Anticancer Properties of Novel Benzophenone‐Conjugated Coumarin Analogs

Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma

Differential genotypical expression of a NEDD9 in normal and tumor tissues: a possible pharmacological target

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