Antiangiogenic Potential of Troxerutin and Chitosan Loaded Troxerutin on Chorioallantoic Membrane Model

Subbaraj, Gowtham Kumar; Elangovan, Harini; Chandramouli, Prema; Yasam, Santhosh Kumar; Chandrasekaran, Kirubhanand; Kulanthaivel, Langeswaran; Pandi, Sangavi; Senthilkumar, Subramanian

doi:10.1155/2023/5956154

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…Maintaining a constant and narrow size distribution is crucial for achieving optimal clinical outcomes with nanocarrier formulations [39]. The size and polydispersity index in the present study was comparatively less than the recently published work with chitosan-loaded troxerutin nanoparticles, in which the size and polydispersity index was 692 d•nm and 0.355, respectively [30]. Both particle size and PDI were expected to increase with the addition of lipid components to the formulations [41,42].…”

Section: Discussionmentioning

confidence: 55%

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Formulation and Characterization of Solid Lipid Nanoparticles Loaded with Troxerutin

Jamous,

Altwaijry,

Saleem

et al. 2023

Processes

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Troxerutin (TXR), a naturally derived compound with diverse therapeutic potential, faces limitations in clinical efficacy due to poor bioavailability and rapid plasma clearance. This study focuses on troxerutin-loaded solid lipid nanoparticles (TXR-SLNs) and their physicochemical properties, intending to enhance drug release. TXR-SLNs were prepared via high-shear homogenization followed by ultrasonication, yielding optimized nanoparticles with an average size of 140.5 ± 1.02 nm, a uniform distribution (polydispersity index: 0.218 ± 0.01), and a stable emulsion (zeta potential: 28 ± 8.71 mV). The formulation exhibited 83.62% entrapment efficiency, indicating improved drug-loading capacity and extended drug release. Spectroscopic and thermodynamic analyses confirmed component compatibility. Despite a decline in entrapment efficiency induced by temperature after one month of storage at 23 °C, the formulation may retain acceptable stability. This study provides insight into SLNs as effective carriers for enhancing troxerutin’s release profile, motivating further in vivo investigations to optimize therapeutic interventions.

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Section: Discussionmentioning

confidence: 55%

“…Recent studies have reported the potential pharmacological benefits of troxerutinloaded nanoparticles by using various polymers like chitosan and selenium [30,31]. In the present study, we aimed to establish the application of TXR loading within nanoparticles by adopting simple methods.…”

Section: Introductionmentioning

confidence: 99%

Formulation and Characterization of Solid Lipid Nanoparticles Loaded with Troxerutin

Jamous,

Altwaijry,

Saleem

et al. 2023

Processes

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“…The importance of MMPs in protein degradation can be evidenced by their wide distribution in different groups of vertebrates [44], including mammals [6][7][8][9], birds [45], reptiles [46,47], amphibians [48] and fish [10], as well as insects [49] and echinoderms [50].…”

Section: Discussionmentioning

confidence: 99%

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

de Oliveira,

Kotowski,

Sampaio-Filho

et al. 2023

Biomedicines

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In dentistry, various animal models are used to evaluate adhesive systems, dental caries and periodontal diseases. Metalloproteinases (MMPs) are enzymes that degrade collagen in the dentin matrix and are categorized in over 20 different classes. Collagenases and gelatinases are intrinsic constituents of the human dentin organic matrix fibrillar network and are the most abundant MMPs in this tissue. Understanding such enzymes’ action on dentin is important in the development of approaches that could reduce dentin degradation and provide restorative procedures with extended longevity. This in silico study is based on dentistry’s most used animal models and intends to search for the most suitable, evolutionarily close to Homo sapiens. We were able to retrieve 176,077 mammalian MMP sequences from the UniProt database. These sequences were manually curated through a three-step process. After such, the remaining 3178 sequences were aligned in a multifasta file and phylogenetically reconstructed using the maximum likelihood method. Our study inferred that the animal models most evolutionarily related to Homo sapiens were Orcytolagus cuniculus (MMP-1 and MMP-8), Canis lupus (MMP-13), Rattus norvegicus (MMP-2) and Orcytolagus cuniculus (MMP-9). Further research will be needed for the biological validation of our findings.

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“…Furthermore, troxerutin protected HacaTcells from UVB-induced decrease in cell growth through regulated miRNA function and suppressed apoptosis ( 25 ). In addition, TRX can reduce Edema and capillary hydrostatic pressure through hyaluronidase and histamine syntheses by TRX application, resulting in the protection of vascular endothelial cells and the effective improvement of microcirculation ( 26 ). Notably, Histamine, 5-hydroxytryptamine, and kinin in venom of jellyfish can operate to contract and spasm vascular smooth muscle and increase the permeability of nearby blood vessels, causing severe discomfort and localized skin congestion and edema ( 7 , 27 ).…”

Section: Introductionmentioning

confidence: 99%

Troxerutin suppress inflammation response and oxidative stress in jellyfish dermatitis by activating Nrf2/HO-1 signaling pathway

Liu,

Wang,

Kuai

et al. 2024

Front. Immunol.

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BackgroundStomolophus meleagris envenomation causes severe cutaneous symptoms known as jellyfish dermatitis. The potential molecule mechanisms and treatment efficiency of dermatitis remain elusive because of the complicated venom components. The biological activity and molecular regulation mechanism of Troxerutin (TRX) was firstly examined as a potential treatment for jellyfish dermatitis. MethodsWe examined the inhibit effects of the TRX on tentacle extract (TE) obtained from S. meleagris in vivo and in vitro using the mice paw swelling models and corresponding assays for Enzyme-Linked Immunosorbent Assay (ELISA) Analysis, cell counting kit-8 assay, flow cytometry, respectively. The mechanism of TRX on HaCaT cells probed the altered activity of relevant signaling pathways by RNA sequencing and verified by RT-qPCR, Western blot to further confirm protective effects of TRX against the inflammation and oxidative damage caused by TE. ResultsTE significantly induced the mice paw skin toxicity and accumulation of inflammatory cytokines and reactive oxygen species in vivo and vitro. Moreover, a robust increase in the phosphorylation of mitogen-activated protein kinase (MAPKs) and nuclear factor-kappa B (NF-κB) signaling pathways was observed. While, the acute cutaneous inflammation and oxidative stress induced by TE were significantly ameliorated by TRX treatment. Notablly, TRX suppressed the phosphorylation of MAPK and NF-κB by initiating the nuclear factor erythroid 2-related factor 2 signaling pathway, which result in decreasing inflammatory cytokine release. ConclusionTRX inhibits the major signaling pathway responsible for inducing inflammatory and oxidative damage of jellyfish dermatitis, offering a novel therapy in clinical applications.

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Antiangiogenic Potential of Troxerutin and Chitosan Loaded Troxerutin on Chorioallantoic Membrane Model

Cited by 9 publications

References 46 publications

Formulation and Characterization of Solid Lipid Nanoparticles Loaded with Troxerutin

Formulation and Characterization of Solid Lipid Nanoparticles Loaded with Troxerutin

Metalloproteinases in Restorative Dentistry: An In Silico Study toward an Ideal Animal Model

Troxerutin suppress inflammation response and oxidative stress in jellyfish dermatitis by activating Nrf2/HO-1 signaling pathway

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