Antibacterial Action of Zinc Oxide Nanoparticles against Staphylococcus aureus in Broiler Breast Fillet

Abstract

Zinc oxide can be called a multifunctional material thanks to its unique physical and chemical properties. Practical application of zinc oxide nanoparticles (ZnO NPs) suspension with different concentrations (5, 8, and 10 mM) were investigated against Staphylococcus aureus artificially inoculated into broiler breast fillets. The results indicated that ZnO suspensions (5, 8 and 10 mM) had a significant inhibitory effect on the growth of Staphylococcus aureus during 12 days of refrigerator storage at 4ºC. Accurately, ZnO NPs 10 mM showed the highest reduction percentage (25.35%) to Staphylococcus aureus from 8.6 to 6.42 log cfu/g, compared to other concentrations (5 and 8 mM). Thus, that the antibacterial activity of ZnO was concentration dependent.

Evaluation of the Antimicrobial Activity of ZnO Nanoparticles against Enterotoxigenic Staphylococcus aureus

“…Our data demonstrated that the highest inhibitory action of ZnO NPs was achieved by smaller size 20 nm with a higher concentration 10 mM against 10 5 CFU/mL S. aureus . The results were concordant with Saafan et al [ 40 ], who reported that size 20 nm achieved a high growth reduction 97.49% and 99.10% for S. aureus with 5 and 10 mM ZnO NPs, respectively at 24 h. However, decreased outcomes were achieved by Ibrahim et al [ 41 ], who reported that the highest growth reduction for S. aureus (10 7 CFU/mL) treated with a concentration 10 mM at sizes 50 and 20 nm ZnO NPs was 12.56% and 25.35%, respectively. The results here with Mirhosseini and Firouzabadi [ 38 ] showed that 20–25 nm at 5 mM ZnO NPs had a 33.9% growth decrease, which is lower than our results, while showing agreement with significant growth inhibition by 10 mM, which nearly totally stopped the growth of 10 7 CFU/mL S. aureus at 24 h. The probable mechanisms of action of metal nanoparticles could be: (a) excessive generation of reactive oxygen species within bacteria, (b) disturbance of key enzymes in the respiratory chain through microbial plasma membranes damage, (c) metal ion collecting in microbial membranes, (d) electrostatic attraction among nanoparticles of metal and microbial cells inhibits metabolic processes, and (e) suppression of microbial proteins/enzymes through improved production of H2O2 [ 42 ].…”

Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)

“…Based on this result, ZnO nanoparticles have an antibacterial effect against E. coli O26.. Narayanan et al (2012) reported that even low concentrations of ZnO NPs had strong antibacterial activity on pathogenic E. coli. While some researchers argued that the antibacterial concentrations of ZnO NPs were safe to a certain level but toxic at higher concentrations to pathogenic E. coli (Bratz et al, 2013;Ibrahim et al, 2017). However, Sikora et al (2018) and Matula et al (2019) found that bacteria could regrow and that the nano-rods were not as strong as the antibiotic treatment because the used strain showed several problems while studying.…”