Antibacterial activities of bark extracts from Fraxinus rhynchophylla Hance and Geranium koreanum Kom. against clinical strains of Clostridium perfringens in chickens

Abstract

: Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compounds for preventing NE. We compared antibacterial activities using two methods and evaluated susceptibilities of the isolates based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Two (Fraxinus rhynchophylla Hance [FRH] and Geranium koreanum Kom.[GKK]) of the 30 plant extracts had potent antibacterial activities against C. perfringens ATCC 13124 in two assays. The MIC values for FRH and GKK against 20 C. perfringens isolates were 128~256 µg/mL and 32~128 µg/mL, respectively. The geometric MIC mean values for the two extracts were 147.2 µg/mL and 68.8 µg/mL, respectively. The MBCs for the two extracts against the same strains were 1,024~2,048 µg/mL and 256~1,024 µg/mL, respectively. The geometric mean MIC and MBC for GKK were about two-fold lower than those of FRH. The modified spot-on-lawn assay may be useful for measuring primary antibacterial potential. FRH and GKK are expected to be used as feed additives to prevent or treat NE in veterinary practice.

Dichloromethane Extracts of Geranium Koreanum Kom. Alleviates Esophagus Damage in Acute Reflux Esophagitis-Induced Rats by Anti-Inflammatory Activities

“…is perennial plant from the Geraniaceae family and grows the Korean and northeast China. Geranium species have several phenolic compounds and commonly used as a conventional medicine to treat a various injury and symptoms such as diarrhea, itching, stomachic, intestinal inflammation and liver disorder, and antibacterial and hepatoprotective effects of Geranium koreanum extracts have been demonstrated [ 14 , 15 , 24 , 25 ]. Geraniin is the main polyphenolic compounds isolated from various Geranium species and is an important Chinese herbal medicine [ 26 ].…”

Bioactivity of the Geranium Genus: A Comprehensive Review

“…25 Trolox (EC50 = 18 µg/mL) was used as a reference compound. 26 Trolox (EC50 = 23 µg/mL) was used as reference compound. 27 Trolox (EC50 = 41 µg/mL) was used as reference compound.…”

Geranium tuberosum Metanol Ekstraktının Enzim İnhibitör Özellikleri ve Antimikrobiyal Aktivitesi

“…It is possible to find many studies examining the various biological characteristics of a big amount of this genus in the literature. For example, ringworm, diarrhea, hypotensive agent, central depression, cancer, cardiovascular, skin, gastrointestinal ulcers, antibacterial, and antiviral activities have been reported ( Kosuge et al, 1985;Ivancheva & Stantcheva, 2000;Chalchat et al, 2002;Williamson, 2002;González-Tejero et al, 2008;Ngezahayo et al, 2015;Oh et al, 2015;).…”