Antibacterial activities of physiologically stable, self-assembled peptide nanoparticles

Nazeer, Nauman; Simmons, Jeffrey R.; Rainey, Jan K.; Rodríguez-Lecompte, J. C.; Ahmed, Marya

doi:10.1039/d1tb01864g

Cited by 9 publications

(19 citation statements)

References 43 publications

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“…The antibacterial activity of WT and mutant Ang4 against S. typhimurium LT2 was examined by colony count and growth curve assays, as described previously ( Doolin et al, 2020 ; Nazeer et al, 2021b ). Briefly, overnight culture of S. typhimurium LT2 was diluted in Luria broth (LB) medium (1:100) and incubated at 37°C until the culture reached the log phase of bacterial growth, which was subsequently washed three times with PBS and diluted to 2 × 10 6 CFU/ml in PBS.…”

Section: Methodsmentioning

confidence: 99%

Identification of Crucial Amino Acid Residues for Antimicrobial Activity of Angiogenin 4 and Its Modulation of Gut Microbiota in Mice

et al. 2022

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Angiogenin 4 bearing ribonuclease activity is an endogenous antimicrobial protein expressed in small and large intestine. However, the crucial amino acid residues responsible for the antibacterial activity of Ang4 and its impact on gut microbiota remain unknown. Here, we report the contribution of critical amino acid residues in the functional regions of Ang4 to its activity against Salmonella typhimurium LT2 and the effect of Ang4 on gut microbiota in mice. We found that Ang4 binds S. typhimurium LT2 through two consecutive basic amino acid residues, K58 and K59, in the cell-binding segment and disrupts the bacterial membrane integrity at the N-terminal α-helix containing residues K7 and K30, as evidenced by the specific mutations of cationic residues of Ang4. We also found that the RNase activity of Ang4 was not involved in its bactericidal activity, as shown by the H12 mutant, which lacks RNase activity. In vivo administration of Ang4 through the mouse rectum and subsequent bacterial 16S rRNA gene sequencing analyses demonstrated that administration of Ang4 not only increased beneficial bacteria such as Lactobacillus, Akkermansia, Dubosiella, Coriobacteriaceae UCG-002, and Adlercreutzia, but also decreased certain pathogenic bacteria, including Alistipes and Enterohabdus, indicating that Ang4 regulates the shape of gut microbiota composition. We conclude that Ang4 kills bacteria by disrupting bacterial membrane integrity through critical basic amino acid residues with different functionalities rather than overall electrostatic interactions and potentially maintains gut microflora in vivo under physiological and pathophysiological conditions.

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Section: Methodsmentioning

confidence: 99%

Identification of Crucial Amino Acid Residues for Antimicrobial Activity of Angiogenin 4 and Its Modulation of Gut Microbiota in Mice

et al. 2022

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“…Nanoparticles can range from nanospheres with hollow cores to a wide variety of solid structures composed of different building blocks [ 105 ]. For example, there is a self-assembled host defense protein-derived peptide, in the form of a β-sheet and a β-turn, which self-assembles to form nanoparticles that can exhibit better antibacterial effects [ 106 ]. Fan and colleagues modified fluorescent nanoparticles (f-PNPs) assembled from cyclic peptides with RGD peptides and then implanted epirubicin (EPI) into the nanoparticles to form a nanocouple (RGD-f-PNPs/EPI).…”

Section: Self-assembled Peptide Nanostructures and Their Application ...mentioning

confidence: 99%

Advances in Self-Assembled Peptides as Drug Carriers

et al. 2023

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In recent years, self-assembled peptide nanotechnology has attracted a great deal of attention for its ability to form various regular and ordered structures with diverse and practical functions. Self-assembled peptides can exist in different environments and are a kind of medical bio-regenerative material with unique structures. These materials have good biocompatibility and controllability and can form nanoparticles, nanofibers and hydrogels to perform specific morphological functions, which are widely used in biomedical and material science fields. In this paper, the properties of self-assembled peptides, their influencing factors and the nanostructures that they form are reviewed, and the applications of self-assembled peptides as drug carriers are highlighted. Finally, the prospects and challenges for developing self-assembled peptide nanomaterials are briefly discussed.

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“…For example, the self‐assembled nanospheres (DSNs) were encapsulated by β‐CD (β‐cyclodextrin) forming polymers (β‐CD‐DSNs) with antimicrobial properties. The polymers exhibited low cytotoxicity, high cellular uptake, faster bacterial flocculation and excellent antibacterial effect against Gram‐negative and Gram‐positive bacteria [29] . A functionalized peptide ACA‐PA derived from an antimicrobial heparin‐binding Cardin motif peptide (AKKARK) 2 could self‐assemble into nanoparticles, which destroy the local cell membrane of bacteria, resulting in bacterial death [30] .…”

Section: Applications Of Peptide‐based Self‐assemblymentioning

confidence: 99%

“…The polymers exhibited low cytotoxicity, high cellular uptake, faster bacterial flocculation and excellent antibacterial effect against Gram-negative and Gram-positive bacteria. [29] A functionalized peptide ACA-PA derived from an antimicrobial heparin-binding Cardin motif peptide (AKKARK) 2 could self-assemble into nanoparticles, which destroy the local cell membrane of bacteria, resulting in bacterial death. [30] Malhotra et al constructed two hybrid antimicrobial peptides, Boc-D-Phe-γ 4 -L-Phe-PEA (NH007) and Boc-L-Phe-γ 4 -L-Phe-PEA (NH009), which can self-assemble into hydrogels.…”

Section: Antibacterial and Hemostasismentioning

confidence: 99%

Self‐Assembling Peptide‐Based Functional Biomaterials

Huo

Yin

et al. 2022

ChemBioChem

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Peptides can self-assemble into various hierarchical nanostructures through noncovalent interactions and form functional materials exhibiting excellent chemical and physical properties, which have broad applications in bio-/nanotechnology. The self-assembly mechanism, self-assembly morphology of peptide supramolecular architecture and their various applications, have been widely explored which have the merit of biocompatibility, easy preparation, and controllable function-ality. Herein, we introduce the latest research progress of selfassembling peptide-based nanomaterials and review their applications in biomedicine and optoelectronics, including tissue engineering, anticancer therapy, biomimetic catalysis, energy harvesting. We believe that this review will inspire the rational design and development of novel peptide-based functional bio-inspired materials in the future.

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Antibacterial activities of physiologically stable, self-assembled peptide nanoparticles

Abstract: In this study, we report that host defense protein-derived ten amino acid long disulfide-linked peptides self-assemble in the form of β-sheets and β-turns, and exhibit concentration-dependent self-assembly in the form...

Cited by 9 publications

References 43 publications

Identification of Crucial Amino Acid Residues for Antimicrobial Activity of Angiogenin 4 and Its Modulation of Gut Microbiota in Mice

Identification of Crucial Amino Acid Residues for Antimicrobial Activity of Angiogenin 4 and Its Modulation of Gut Microbiota in Mice

Advances in Self-Assembled Peptides as Drug Carriers

Self‐Assembling Peptide‐Based Functional Biomaterials

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