Antibacterial activities of the essential oil and hydroethanolic extract from Aeollanthus heliotropioides Oliv

Nguimatsia, François; Kenmogne, Sidonie Beatrice; Ngo-Mback, M.N.L.; Kouamouo, Jonas; Tchuitio, Léa Larissa Nzenti; Dongmo, Yannick Kevin Melogmo; Dongmo, Pierre Michel Jazet

doi:10.13171/mjc02102021555mnlnm

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…To study the bacterial growth curve, cultures containing E. coli and S. aureus strains were incubated with the most active antibacterial nanoparticles (AgNPs) and nanocomposites (CAC/AgNPs) as per the protocol reported by Nguimatsia et al [76] with minor modifications. In short, 20 µL of AgNPs and 8 µL of CAC/AgNPs were added to 180 µL and 192 µL of Mueller Hinton Broth, respectively, in the first twelve wells of column A (A).…”

Section: Bacterial Growth Kinetics Resulting From Various Concentrati...mentioning

confidence: 99%

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

Tchinda Taghu,

Pone Kamdem,

Ngouana

et al. 2023

DDC

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The development of antimicrobial resistance has increased the prevalence of infectious diseases, causing a global health problem that accounts for over 4.95 million deaths worldwide annually. The side effects associated with current antibiotics prompt a crucial need to search for effective and safe antimicrobial agents. In this study, silver nanoparticles (AgNPs) were prepared by chemical reduction method using silver nitrates as a metallic precursor and Croton macrostachyus bark aqueous extract as a reducing and capping agent. The nanoparticles were further functionalized using C. macrostachyus-based activated carbon (CAC) to generate nanocomposites (CAC-AgNPs). The nanomaterials were characterized by ultraviolet-visible (UV–vis) absorption spectra and Fourier transform infrared (FTIR) spectra. The antibacterial activity of the as-prepared nanomaterials was evaluated against an array of bacterial strains by microdilution method, whereas their cytotoxicity profile was evaluated using Vero cells (human mammalian cells). Antibacterial mechanistic studies of active nanomaterials were carried out through bacterial growth kinetics, nucleic acid leakage tests, and catalase inhibition assays. As a result, the as-prepared nanomaterials exhibited antibacterial activity against an array of bacterial strains (minimum inhibitory concentration (MIC) range: 62.5 to 500 µg/mL), the most susceptible being Escherichia coli and Staphylococcus aureus. Cytotoxicity studies of the nanomaterials on Vero cells revealed that the nanocomposite (median cytotoxic concentration (CC50): 213.6 µg/mL) was less toxic than its nanoparticle (CC50 value: 164.75 µg/mL) counterpart. Antibacterial mechanistic studies revealed that the nanomaterials induced (i) bacteriostatic activity vis à vis E. coli and S. aureus and (ii) inhibition of catalase in these bacteria. This novel contribution regarding the antibacterial mechanisms of action of silver nanocomposites from C. macrostachyus-based activated carbon may contribute to our understanding of the antibacterial action of these biomaterials. Nevertheless, more chemistry and in vivo experiments as well as in depth antibacterial mechanistic studies are warranted for the successful utilization of these antibacterial biomaterials.

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Section: Bacterial Growth Kinetics Resulting From Various Concentrati...mentioning

confidence: 99%

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

Tchinda Taghu,

Pone Kamdem,

Ngouana

et al. 2023

DDC

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“…The time-kill kinetic study of the most active nanoparticles and nanocomposites was performed on E. coli according to the method described by Nguimatsia et al [18]. Herein, the active nanomaterials were prepared at various concentrations (MIC, 2MIC and 4MIC), by two-fold serial dilutions in a 96-well microplate.…”

Section: Time-kill Kinetics Testmentioning

confidence: 99%

“…In addition, contemporary medicines derive basically from herbs with reference to traditional knowledge, practices, and beliefs. According to the World Health Organization (WHO), more than 80% of the world's population relies on traditional medicine for their basic health care [18]. One such plant is Cocos nucifera, which is used for the traditional treatment of diarrhea and stomach aches, and as a topic ointment for dermatitis, abscesses, injuries, etc.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Cocos nucifera-Based Nanomaterials and Mechanistic Basis of Their Antimicrobial Action

Yajeh Tanka,

Ankoro,

Ngouana

et al. 2024

BioMed

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Caused by pathogenic microorganisms, infectious diseases are known to cause high mortality rates, severe burdens of disability, and serious worldwide aftermaths. Drug-resistant pathogens have reduced the efficacy of available therapies against these diseases, thus accentuating the need to search for effective antimicrobials. Medicinal plants have served as starting material for the preparation of a number of antimicrobial agents. To this end, the present study highlights the green synthesis of Cocos nucifera-based nanomaterials and evaluation of the mechanistic basis of their antimicrobial action. Accordingly, Cocos nucifera extract was used for the reduction of silver nitrate solution to afford silver nanoparticles. These entities were further incorporated onto sulfuric-acid-based activated carbons to generate the nanocomposites. The antimicrobial activity of the as-prepared nanomaterials was evaluated using the broth microdilution method, while the antioxidant activity was assessed through standard methods. The cytotoxicity of potent nanomaterials was assessed on Vero cells by the spectrophotometric method. As a result, nanoparticles were successfully synthesized, as evidenced by the ultraviolet–visible spectroscopy analysis that revealed an intense absorption spectrum at 433 nm. Fourier Transform Infrared Spectroscopy presented the functional group moieties involved as a capping and reducing agent in the synthesis of the nanomaterials. The incubation of nanomaterials with selected bacterial and fungal strains has led to significant inhibitory effects of these pathogens with minimum inhibitory concentrations ranging from 7.813 to 250 μg/mL. In antioxidant assays, the nanocomposites presented scavenging activities comparable to those of ascorbic acid. Cytotoxicity experiment revealed no toxic effects on Vero cells (range of selectivity indices: from >4 to >128). These results provide evidence of the implication of Cocos nucifera-based nanomaterials in targeting bacterial or fungal systems that mediate free-radical damage or by inhibiting the oxidative damage caused by selected bacteria and fungi, the most susceptible being Escherichia coli and Candida albicans, respectively.

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“…To study the bacterial growth curve, cultures containing E. coli and S. aureus strains were incubated with the most active antibacterial nanoparticles (AgNPs) and nanocomposites (CAC/AgNPs) as per the protocol reported by Nguimatsia et al [69] with minor modifications. In short, 20 µL of AgNPs and 8 µL of CAC/AgNPs were added respectively in 180 µL and 192 µL of Mueller Hinton Broth in the first twelve wells of column A (A).…”

Section: Bacterial Growth Kinetics On Various Concentrations Of the N...mentioning

confidence: 99%

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

Tchinda Taghu,

Pone Kamdem,

Ngouana

et al. 2023

Preprint

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In this study, we report the antibacterial mechanisms of action of uniform silver nanoparticles (AgNPs) and decorated activated carbon nanocomposite (CAC-AgNPs) obtained using a green synthesis approach. The nanomaterials were characterized by ultraviolet-visible (UV-vis) absorption spectra and Fourier transform infrared (FTIR) spectra. The antibacterial activity of the as-prepared nanomaterials was evaluated against an array of bacterial strains by microdilution method, whereas their cytotoxicity profile was evaluated on Vero cells (human mammalian cells). The antibacterial mechanistic studies of active nanomaterials were carried out through bacterial growth kinetics, nucleic acid leakage test, and catalase inhibition assay. A silver nanocomposite was successfully fabricated from Croton macrostachyus-based activated carbon. The as-prepared nanomaterials exhibited antibacterial activity against an array of bacterial strains (minimum inhibitory concentration (MIC) range: 62.5 to 500 µg/mL), the most susceptible being Escherichia coli and Staphylococcus aureus. Cytotoxicity studies of the nanomaterials on Vero cells revealed that the nanocomposite (median cytotoxic concentration (CC50): 213.6 µg/mL) was less toxic than the nanoparticles (CC50 value: 164.75 µg/mL) counterpart. Antibacterial mechanistic studies unveiled that the nanomaterials induced (i) bacteriostatic activity vis-à-vis E. coli and S. aureus and (ii) inhibition of catalase in these bacteria. This novel contribution on the antibacterial mechanisms of action of silver nanocomposite from C. macrostachyus-based activated carbon might contribute to the understanding of antibacterial action of these biomaterials. Nevertheless, more chemistry and in vivo experiments, as well as in depth antibacterial mechanistic studies are warranted for the successful utilization of these antibacterial biomaterials.

show abstract

Antibacterial activities of the essential oil and hydroethanolic extract from Aeollanthus heliotropioides Oliv

Cited by 4 publications

References 25 publications

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

Green Synthesis of Cocos nucifera-Based Nanomaterials and Mechanistic Basis of Their Antimicrobial Action

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

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