Antibacterial activity against resistant bacteria and cytotoxicity of four alkaloid toxins isolated from the marine sponge Arenosclera brasiliensis

Torres, Yohandra Reyes; Berlinck, Roberto G. S.; Nascimento, Gislene Garcia Franco do; Fortier, Sérgio Costa; Pessoa, Cláudia; Moraes, Manoel Odoríco de

doi:10.1016/s0041-0101(01)00286-0

Cited by 67 publications

(59 citation statements)

References 30 publications

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“…Although Brazil has the world's second most extensive coastline after Australia, the development of the chemistry and pharmacology of Brazilian marine organisms has been hampered for many years because the main focus of Brazilian natural product chemists has been directed to the study of medicinal plants and microorganisms. Only recently, a few Brazilian research groups have focused on the chemistry of marine organisms (Torres et al, 2002;Berlinck et al, 2004;Seleghim et al, 2007). Our results add new information to the growing database of known biological activities of Brazilian sponges.…”

Section: Discussionmentioning

confidence: 60%

“…Seven out of twelve sponges from Rio de Janeiro (58.3%) were active against such resistant bacteria, a high percentage when compared with other studies. Probably, the methodologies used by other authors (e.g., Monks et al, 2002;Torres et al, 2002 andSeleghim et al, 2007) may be not as sufficiently sensitive as the methodology described by Giambiagi-deMarval et al, (1990), where each crude extract of sponge was spotted on the surface of agar, instead of using filter paper discs impregnated with the crude extracts. The general problems inherent to antimicrobial screening of plant extracts have been reviewed by Cos et al, (2006).…”

Section: Discussionmentioning

confidence: 99%

“…The Arenosclerins A-C and haliclonacyclamine E isolated from the Brazilian sponge Arenosclera brasiliensis inhibited growth of twelve antibiotic-resistant bacteria isolated from a hospital (Torres et al, 2002). Crude extracts of less than 3% of 215 Brazilian sponge species tested showed activity against antibiotic-resistant bacteria (Seleghim et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Up to 800 antibiotic compounds have been isolated from marine sponges (Torres et al, 2002). In the continuing effort by the marine natural products community, many antibacterial agents have been identified from sponges.…”

Section: Introductionmentioning

confidence: 99%

“…So far, pure compounds isolated from one specie (Torres et al, 2002) and crude extracts of 215 species of Brazilian sponges have been investigated through bioassays against antibiotic-resistant bacteria (Seleghim et al, 2007). Although a growing number of studies investigated the cytotoxic, neurotoxic and antimicrobial activities of sponges from the Brazilian coast (Muricy et al, 1993;Rangel et al, 2001;Monks et al, 2002;Berlinck et al, 2004;Seleghim et al, 2007), most Brazilian sponges remain unstudied.…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Marinho¹,

Muricy²,

Silva³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Bactérias resistentes a antibióticos inibidas por extratos e frações de esponjas marinhas do Brasil". O número crescente de bactérias resistentes aos antibióticos tem se tornado um sério problema médico nos últimos anos. As esponjas marinhas são uma fonte rica em compostos bioativos e muitas espécies podem ser úteis para o desenvolvimento de novos antimicrobianos. Esse estudo descreve uma triagem in vitro de esponjas para a pesquisa de novas substâncias contra bactérias resistentes. Os extratos de esponjas foram testados sobre 44 estirpes bacterianas, incluindo quatorze resistentes a antibióticos. Dez entre doze espécies de esponjas apresentaram atividade em um ou mais bioensaios. Os extratos aquosos de Cinachyrella sp. e Petromica citrina apresentaram um amplo espectro de ação sobre estirpes bacterianas resistentes, tais como, Staphylococcus aureus, Staphylococcus coagulase-negativos e Enterococcus faecalis. O extrato aquoso de P. citrina foi fracionado e a fração aquosa apresentou atividade inibitória sobre estirpes de Staphylococcus. Esta fração, na concentração do CMI (16 μg/mL), demonstrou efeito bactericida sobre células de S. aureus na fase exponencial de crescimento. O mecanismo de ação da fração ainda não foi elucidado, mas nós observamos que esta afeta a síntese protéica de Staphylococcus. Nossos resultados demonstraram pela primeira vez que Petromica citrina é uma fonte potencial de novas drogas para o tratamento de infecções causadas por bactérias resistentes. Unitermos: atividade antibacteriana, bactéria resistente a antibióticos, esponjas marinhas, Petromica citrina. ABSTRACT:The growing number of bacterial strains resistant to conventional antibiotics has become a serious medical problem in recent years. Marine sponges are a rich source of bioactive compounds, and many species can be useful for the development of new antimicrobial drugs. This study reports the in vitro screening of marine sponges in the search for novel substances against antibiotic-resistant bacteria. Sponge extracts were tested against 44 bacterial strains, including fourteen antibiotic-resistant strains. Ten out of the twelve sponge species studied showed activity in one or more of the bioassays. Aqueous extracts of Cinachyrella sp. and Petromica citrina showed a large action spectrum over resistant-bacteria such as Staphylococcus aureus, coagulase-negative staphylococci and Enterococcus faecalis. Aqueous extract of P. citrina was fractioned and aqueous fraction showed a greatest inhibitory activity on Staphylococcus strains. In addition, this fraction demonstrated a bactericidal effect on exponentially growing S. aureus cells at the MIC (16 µg/ mL). The mechanism of action of bioactive fraction is still unclear, but we showed that it affect protein biosynthesis of Staphylococcus. Our results demonstrated for the first time that P. citrina is a potential source of new drugs for the treatment of infections by antibiotic-resistant bacteria.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Marinho¹,

Muricy²,

Silva³

et al. 2010

Rev. bras. farmacogn.

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Porifera (Sponges): Recent Knowledge and New Perspectives

Renard

Gazave

Fierro-Constaín

et al. 2013

Encyclopedia of Life Sciences

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Porifera, commonly named sponges, are true animals or metazoan despite their anatomical and morphological simplicity that had led to a long‐time debate about their nature (animal versus vegetal) and constitution (colonies of unicellular organisms versus multicellular organisms). Sponges are a successful group of mostly marine filter feeder organisms that represent a major life form of several ecosystems. Devoid of organs, the main characteristics of their body plan are the presence of a network of pores (at the origin of their name), choanocyte chambers and canals devoted to water filtration and spicules (when present) allowing tissue physical support. Currently considered as the sister group to all the other animals, these organisms have a key phylogenetic position. Recent studies have shown that they possess an unexpected molecular complexity raising exciting questions about early animal evolution. Key Concepts: As adults, sponges are sedentary filter feeder animals. The water flow is canalised in an aquiferous system composed of pores (ostia), canals and choanocyte chambers. At present, the number of formally described sponge species is comprised between 8000 and 9000. Sponges are divided into four clades […] Hexactinellida, Demospongiae, Calcarea and Homoscleromorpha. As sponges are devoid of organs, their individual cells or cell layers ensure vital functions. One cell type may have several functions. In addition to cell plurifunctionality, the functional plasticity of sponges also relies on a high capacity of cell transdifferentiation. Sponges often use both asexual and sexual reproduction. Most of key transcription families and main signalling pathways required for eumetazoan development and body patterning are present in sponges. The recent rising interest for Porifera is expected to lead to a better understanding of animal evolution.

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Brominated Diphenyl Ethers Including a New Tribromoiododiphenyl Ether from the Vietnamese Marine Sponge Arenosclera sp. and Their Antibacterial Activities

Awouafack

Wong

et al. 2019

Chemistry & Biodiversity

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A new tribromoiododiphenyl ether (1) and eight known brominated diphenyl ethers (2–9) were isolated from the MeOH extract of the sponge Arenosclera sp. collected in Vietnam, using repeated open column chromatography and preparative thin layer chromatography. The chemical structure of the new compound 1 was determined by analyses of spectroscopic (1D‐ and 2D‐NMR, and MS) data and by comparison of our data with those reported in the literature. Compounds 1, 3, and 8 exhibited strong antibacterial activities against the Gram‐positive bacteria Bacillus subtilis and Staphylococcus aureus and the Gram‐negative bacterium Klebsiella pneumoniae with MIC values ranging from 0.8 to 6.3 μm, while compounds 5 and 7 only displayed activities against Gram‐positive bacteria with MIC values from 0.5 to 3.1 μm. Compound 2 showed activities against the four tested bacteria with MIC values ranging from 0.5 to 6.3 μm.

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Antibacterial activity against resistant bacteria and cytotoxicity of four alkaloid toxins isolated from the marine sponge Arenosclera brasiliensis

Cited by 67 publications

References 30 publications

Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Porifera (Sponges): Recent Knowledge and New Perspectives

Brominated Diphenyl Ethers Including a New Tribromoiododiphenyl Ether from the Vietnamese Marine Sponge Arenosclera sp. and Their Antibacterial Activities

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