Antibacterial Activity Against Streptococcus Pneumoniae by Mouse Lung Lymphocytes

Sestini, Piersante; Nencioni, L; Villa, L; Boraschi, Diana; Tagliabue, Aldo

doi:10.1007/978-1-4684-5344-7_61

Cited by 8 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ASCs are responsible for recall immune responses that confer protection against infection. The generation of IgA ASCs in the lung is important to fight against S. pneumoniae infection since lungs are the primary invasion sites of S. pneumoniae, and mouse lung has IgA-bearing lymphocytes that help against pneumococcal infection through an IgA-driven mechanism (91). The higher IgA ASC responses in lungs at 7 weeks were correlated with the slightly enhanced IgA responses in vaginal washes at 6 and 8 weeks (Fig.…”

Section: Resultsmentioning

confidence: 87%

A Colanic Acid Operon Deletion Mutation Enhances Induction of Early Antibody Responses by Live Attenuated Salmonella Vaccine Strains

Wang

Shi

et al. 2013

Infect Immun

View full text Add to dashboard Cite

bColanic acid (CA) is a common exopolysaccharide produced by many genera in the Enterobacteriaceae. It is critical for biofilm formation on HEp-2 cells and on chicken intestinal tissue by Salmonella. In this study, we generated different CA synthesis gene mutants and evaluated the immune responses induced by these mutants. One of these mutations, ⌬(wza-wcaM)8, which deleted the whole operon for CA synthesis, was introduced into two Salmonella vaccine strains attenuated by auxotrophic traits or by the regulated delayed attenuation strategy (RDAS). The mice immunized with the auxotrophic Salmonella vaccine strain with the deletion mutation ⌬(wza-wcaM)8 developed higher vaginal IgA titers against the heterologous protective antigen and higher levels of antigen-specific IgA secretion cells in lungs. In Salmonella vaccine strains with RDAS, the strain with the ⌬(wza-wcaM)8 mutation resulted in higher levels of protective antigen production during in vitro growth. Mice immunized with this strain developed higher serum IgG and mucosal IgA antibody responses at 2 weeks. This strain also resulted in better gamma interferon (IFN-␥) responses than the strain without this deletion at doses of 10 8 and 10 9 CFU. Thus, the mutation ⌬(wza-wcaM)8 will be included in various recombinant attenuated Salmonella vaccine (RASV) strains with RDAS derived from Salmonella enterica serovar Paratyphi A and Salmonella enterica serovar Typhi to induce protective immunity against bacterial pathogens.

show abstract

Section: Resultsmentioning

confidence: 87%

A Colanic Acid Operon Deletion Mutation Enhances Induction of Early Antibody Responses by Live Attenuated Salmonella Vaccine Strains

Wang

Shi

et al. 2013

Infect Immun

View full text Add to dashboard Cite

show abstract

“…This assay was conducted in the absence of complement and therefore focuses on events mediated solely by antibody. Even though IgG2a is thought to be the most effective opsonizing antibody in mice (27,36), IgAmediated phagocytosis of S. pneumoniae has actually been demonstrated with murine alveolar macrophages (32). In humans, IgA-mediated phagocytosis by an Fc receptor mechanism was initially described in neutrophils (40).…”

Section: Discussionmentioning

confidence: 99%

Intranasal Vaccination with Pneumococcal Surface Protein A and Interleukin-12 Augments Antibody-Mediated Opsonization and Protective Immunity againstStreptococcus pneumoniaeInfection

et al. 2001

View full text Add to dashboard Cite

Streptococcus pneumoniae is a major pathogen in humans that enters the host primarily through the respiratory tract. Targeting mucosal surfaces directly may therefore be an optimal approach for vaccination to prevent bacterial colonization and invasive disease. We have previously demonstrated the effectiveness of interleukin-12 (IL-12) delivered intransally (i.n.) as an antiviral respiratory adjuvant. In this study, we examined the effects of i.n. IL-12 treatment on induction of protective humoral immunity against S. pneumoniae. Immunization i.n. with pneumococcal surface protein A (PspA) and IL-12 resulted in enhanced lung IL-10 mRNA expression and marked augmentation of respiratory and systemic immunoglobulin G1 (IgG1), IgG2a, and IgA antibody levels compared to those in animals receiving PspA alone. In addition, i.n. vaccination with PspA and IL-12 provided increased protection against nasopharyngeal carriage. Flow cytometric analysis revealed a threefold increase in antibody-mediated, complement-independent opsonic activity in the sera of PspA-and IL-12-treated animals, which was mainly contributed by IgG2a and, to a lesser extent, IgA. Passive transfer of these immune sera conferred complete protection from death upon systemic pneumococcal challenge. These findings demonstrate the effectiveness of combining PspA and IL-12 at mucosal sites to achieve optimal antibody-mediated opsonization and killing of S. pneumoniae.

show abstract

“…(180,181). Similarly, the same group found antibacterial activity against Streptococcus pneumoniae by mouse lung lymphocytes in conjunction with IgA (182). ADCC has also been described for Ehrlichia risticii and Coxiella burnetii-infected cells and for Brucella abortus, although, as with other bacteria, the role, if any, of ADCC in these infections in vivo is unknown (183,184,185).…”

Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 96%

Functions of Antibodies

Forthal

2014

Microbiol Spectr

176

View full text Add to dashboard Cite

Antibodies can impact pathogens in the presence or in the absence of effector cells or effector molecules such as complement, and experiments can often sort out with precision the mechanisms by which an antibody inhibits a pathogen in vitro. In addition, in vivo models, particularly those engineered to knock in or knock out effector cells or effector molecules, are excellent tools for understanding antibody functions. However, it is highly likely that multiple antibody functions occur simultaneously or sequentially in the presence of an infecting organism in vivo. The most critical incentive for measuring antibody functions is to provide a basis for vaccine development and for the development of therapeutic antibodies. In this respect, some functions, such as virus neutralization, serve to inhibit the acquisition of a pathogen or limit its pathogenesis. However, antibodies can also enhance replication or contribute to pathogenesis. This review emphasizes those antibody functions that are potentially beneficial to the host. In addition, this review will focus on the effects of antibodies on organisms themselves, rather than on the toxins the organisms may produce.

show abstract

Antibacterial Activity Against Streptococcus Pneumoniae by Mouse Lung Lymphocytes

Cited by 8 publications

References 19 publications

A Colanic Acid Operon Deletion Mutation Enhances Induction of Early Antibody Responses by Live Attenuated Salmonella Vaccine Strains

A Colanic Acid Operon Deletion Mutation Enhances Induction of Early Antibody Responses by Live Attenuated Salmonella Vaccine Strains

Intranasal Vaccination with Pneumococcal Surface Protein A and Interleukin-12 Augments Antibody-Mediated Opsonization and Protective Immunity againstStreptococcus pneumoniaeInfection

Functions of Antibodies

Contact Info

Product

Resources

About