Antibacterial activity and in vitro anti-tumor activity of the extract of the larvae of the housefly (Musca domestica)

Hou, Lixia; Shi, Yonghui; Zhai, Pei; Li, Guowei

doi:10.1016/j.jep.2006.11.015

Cited by 116 publications

(80 citation statements)

References 15 publications

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“…[2]. However, the immature stages have several industrial and medical applications [3][4][5][6]. Such challenging situation requires a management strategy to interfere with the insect development in order to maintain adult stage population as lower as possible through controlling larval stage population.…”

Section: Introductionmentioning

confidence: 99%

Larvicidal Activity of Some Botanical Extracts, Commercial Insecticides and their Binary Mixtures Against the Housefly, Musca Domestica L.

Mansour¹

2011

TOTNJ

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Following preliminary screening of 13 ethanolic plant extracts, belonging to 10 different families, a total of 11 extracts were subjected to detailed toxicity evaluation against the larval stage of the housefly, Musca domestica L. The larvicidal LC 50 values were < 100 ppm for Piper nigrum (50.1 ppm), Azadirachta indica (76.9 ppm), Conyza aegyptiaca (77.0 ppm) and Cichorium intybus (96.8 ppm); representing the highest potent extracts among the bioassayed candidates. Punica granatum extract exhibited the lowest toxicity (213.9 ppm). In comparison, commercial insecticides showed superior larvicidal toxicity; accounting to the following LC 50 values: 0.029, 0.03, 0.61 and 0.64 ppm for deltamethrin, methomyl, chlorpyrifos and flufenoxuron, respectively. Combining botanical extracts with insecticides, at equitoxic dosages (e.g., LC 25 values), induced potentiating effects for a 44 bioassayed mixtures against the housefly larvae. Moreover, mixing the insecticides at LC 0 (a concentration level causing no observed mortality) with the LC 50 of each of the plant extracts have resulted in 44 paired combinations. Mostly, the "synergistic factor; S.F." ranged between 1.6 -1.9; giving rise to high synergistic effects. Specifically, the synergistic effect was much pronounced for mixtures of the insecticide deltamethrin with different botanical extracts. Most of the tested toxicants induced different forms of developmental effects after exposure of 3 rd larval instars to sublethal concentrations (LC 25 ppm). Larvae treated with A. indica, Citrus aurantifolia, Eucalyptus globulus (leaves or fruits), P. granatum, Salix safsaf, Sonchus oleraceus, Zea mays, as well as the insecticides chlorpyrifos, deltamethrin and methomyl failed to develop into adult stages. Morphologically, there were different forms of pupal and adult abnormalities, where the treatments of P. granatum and S. oleraceus caused abnormal pupal size in addition to pupal-adult intermediate. In the resulted Musca domestica adults, the effects were seen as one-winged insects, small size, compressed body and abdomen elongation. Such deformations were attributed to treatments of C. intybus, C. aegyptiaca, Piper nigrum and the IGR flufenoxuron. The overall results of the present investigation reveal the broad-spectrum toxic properties of the tested plant extracts against Musca domestica larvae; which may encourage further research on housefly control in tropics using indigenous plants.

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Section: Introductionmentioning

confidence: 99%

Larvicidal Activity of Some Botanical Extracts, Commercial Insecticides and their Binary Mixtures Against the Housefly, Musca Domestica L.

Mansour¹

2011

TOTNJ

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“…The most feasible and easiest commercial utilisation of housefly larvae is to raise them on poultry manure and other organic wastes and then feed them fresh to poultry or other livestock. In addition to their value as a livestock feed, other valuable materials, such as protein or peptides, chitosan, phospholipid, and antibiotics have been extracted from housefly larvae (Bridges & Price 1970;Iaboni et al 1998;Hou et al 2007;Ai et al 2008). Our preliminary observations indicate that dried housefly larvae contain approximately 55% protein, 9.1% crude chitin, and 8.8% fat (unpublished data).…”

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confidence: 99%

Preparation and characterisation of food grade chitosan from housefly larvae

Zhang¹,

Qin²,

Zhang³

et al. 2011

Czech J. Food Sci.

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The preparation and characterisation of food-grade chitosan from housefly larvae are reported. A refinement procedure was developed to remove larval mouth hooks from the primary chitosan product, which greatly improved the quality of the final product and simplified the production procedures. Different factors affecting chitosan preparation were studied and an orthogonal experiment was designed to determine optimal preparation conditions. When prepared under optimal reaction conditions, the end product was snow-white in colour, had a high deacetylation percentage, good viscosity, and a low ash content. The end product was characterised by Fourier transform infrared spectral analysis, X-ray diffraction analysis, thermo-gravimetric analysis, and differential scanning calorimetry. Its physical and chemical properties and sanitary index were determined and compared to the relevant Chinese standards. The results show that the chitosan we produced under optimal conditions meets the Chinese Fishery Trade Standard SC/T3403-2004 for food-grade chitosan.

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“…Recently, antioxidant (Zhu et al, 2013) and antibacterial and in vitro antitumor (Hou et al, 2007) activities of the protein extract of the M. domestica maggot have been reported. In our earlier studies, we found that the protein-enriched fraction and the low molecular weight peptide extract of M. domestica maggot could inhibit atherosclerotic lesions (Chu et al, 2011(Chu et al, , 2013.…”

Section: Discussionmentioning

confidence: 99%

Effect ofMusca domesticmaggot polypeptide extract on HUVEC dysfunction induced by early-activated macrophages

Chu

Jin

et al. 2015

Pharmaceutical Biology

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Context: Musca domestica Linn. maggot is a traditional Chinese medicine. In our previous studies, Musca domestica maggot protein-enriched fraction and polypeptide extract (molecular weight 530 kD) were found to reverse endothelial cell dysfunction in atherosclerotic lesions. Objective: This study determines whether and how M. domestica maggot polypeptide extract affects the dysfunction of human umbilical vein endothelial cells (HUVEC) induced by macrophages (M'). Materials and methods: HUVEC and early-activated THP-1 M' (incubated with LPS of 1 mg/ml for 2 h) were co-cultured in a Transwell system. The effects of Musca domestica maggot polypeptide extract (with increasing concentrations, i.e., 1.0, 2.5, 5.0, 10.0, 20.0, and 40.0 mg/ml) on the proliferation and migration HUVEC and their secretion of vascular endothelial growth factor (VEGF) were determined by flow cytometry, modified Boyden chamber assay, and enzyme-linked immunosorbent assay (ELISA) after incubation for 24 h. Results: Musca domestica maggot polypeptide extract decreased the proliferation of HUVEC in a concentration-dependent manner, with a 50% effective concentration (EC 50 ) of 22.16 ± 1.48 mg/ml, and effectively inhibited HUVEC migration (EC 50 ¼ 35.15 ± 2.03 mg/ml) and VEGF secretion (EC 50 ¼ 28.64 ± 1.29 mg/ml). Discussion and conclusion: Musca domestica maggot polypeptide extract can inhibit the dysfunction of HUVEC induced by early-activated THP-1 M'.

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Antibacterial activity and in vitro anti-tumor activity of the extract of the larvae of the housefly (Musca domestica)

Cited by 116 publications

References 15 publications

Larvicidal Activity of Some Botanical Extracts, Commercial Insecticides and their Binary Mixtures Against the Housefly, Musca Domestica L.

Larvicidal Activity of Some Botanical Extracts, Commercial Insecticides and their Binary Mixtures Against the Housefly, Musca Domestica L.

Preparation and characterisation of food grade chitosan from housefly larvae

Effect ofMusca domesticmaggot polypeptide extract on HUVEC dysfunction induced by early-activated macrophages

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