Antibacterial activity and mechanism of lactobionic acid against Staphylococcus aureus

Cao, Jiarong; Fu, Hongjie; Gao, Lihong; Zheng, Yan

doi:10.1007/s12223-019-00705-3

Cited by 33 publications

(27 citation statements)

References 33 publications

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“…The cell integrity of V. Parahemolyticus, S. putrefaciens, S. aureus , and P. fluorescens strains were examined by determining the release of nucleic acids and proteins into supernatant according to Xu et al [24] and Cao et al [25].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage

Fang

Zhou

et al. 2019

Molecules

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The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol against Vibrio Parahemolyticus, Shewanella putrefaciens, Staphylococcus aureus and Pseudomonas fluorescens and evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (Lateolabrax maculatus) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol against V. parahemolyticus, S. putrefaciens, S. aureus and P. fluorescens were 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage

Fang

Zhou

et al. 2019

Molecules

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“…The antibacterial activity of YACTH against SAU was measured by Oxford cup and micro-dilution methods, diameters of inhibition zone (DIZ) were appreciated as follows: not sensitive (diameter ≤ 8.0 mm), moderately sensitive (8.0 < diameter ≤ 14.0 mm), sensitive (14.0 < diameter ≤ 20.0 mm), and extremely sensitive (diameter >20.0 mm) [15]. The mean of DIZ values was 28.04mm (± 0.94 mm), and the corresponding MIC and MBC against SAU were 8.625 mg/mL and 17.25 mg/mL, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Antibacterial activity of YACTH was tested according to the agar diffusion method as reported previously with slight modification [15]. The YACTH extracts were filtered through 0.22 µm sterilizing filters.…”

Section: Methodsmentioning

confidence: 99%

“…CC-BY 4.0 International license made available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is The copyright holder for this preprint this version posted July 22, 2021. ; https://doi.org/10.1101/2021.07.22.453437 doi: bioRxiv preprint micro-dilution methods, diameters of inhibition zone (DIZ) were appreciated as follows: not sensitive (diameter ≤ 8.0 mm), moderately sensitive (8.0 < diameter ≤ 14.0 mm), sensitive (14.0 < diameter ≤ 20.0 mm), and extremely sensitive (diameter >20.0 mm) [15]. The mean of DIZ values was 28.04mm (± 0.94 mm), and the corresponding MIC and MBC against SAU were 8.625 mg/mL and 17.25 mg/mL, respectively.…”

Section: Antibacterial Activity Of Yacthmentioning

confidence: 99%

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TMT-based proteomic analysis of the antibiotic effects of ShangKeHuangShui against Straphylococcus aureus

Liu

Zhao

Yang

et al. 2021

Preprint

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Post-traumatic infection is a serious orthopedic trauma complication commonly caused by Staphylococcus aureus (SAU). ShangKeHuangShui, also known as Yellow Aqueous Concentrate of Traumatology Herbs (YACTH), is prepared from six Chinese herbal medicines, which has been used for decades in our hospital to prevent post-traumatic infection. In the present study, we investigated the in vitro antibacterial effects and underlying mechanism of YACTH against SAU. YACTH exhibited significant antibacterial activity against SAU with the minimum inhibitory concentration (MIC) of 8.625 mg/mL. Proteomic analysis based on Tandem mass tag (TMT) showed different protein expression levels in SAU under the YACTH and control conditions. Compared to the control group, the expression level of 490 proteins of YACTH treated group significantly changed (>1.2 fold, P <0.05). Biological informatics analysis showed that these differential proteins were widely involved in a variety of biological processes and multiple metabolic pathways. We then selected 26 target proteins to further conduct Parallel reaction monitoring (PRM). The results revealed that the 9 down-regulated proteins were mainly involved in RNA polymerase, oxidative phosphorylation, ABC transporter and glycolysis; whereas, the 17 up-regulated proteins are related to RNA degradation, mismatch repair and ribosome. In conclusion, YACTH, which contains the water-soluble components of six Chinese herbs, is first reported to exhibit antibacterial activity against SAU in vitro . The results provide novel insights into the antibacterial mechanisms of YACTH on protein networks, which may help us find new potential antibiotic targets.

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“…To assess the extent of intracellular content release that reflects the permeability of the outer membrane of MRSA, the K + concentration and alkaline phosphatase (ALP) activity were determined respectively. The extracellular K + leakages in the BER-treated and control cells were assayed after 9 h, as described previously (Wu et al, 2018), and the ALP activity in the culture broth was evaluated using an ALP kit (Nanjing Jiancheng Institute of Bioengineering, Nanjing, Jiangsu, China) and a microplate reader (Eon, Biotek, United States) (Cao et al, 2019;Kang et al, 2020).…”

Section: Detection Of K + and Alkaline Phosphatase Leakagesmentioning

confidence: 99%

Berberine Damages the Cell Surface of Methicillin-Resistant Staphylococcus aureus

Zhang

Sun

et al. 2020

Front. Microbiol.

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Methicillin-resistant Staphylococcus aureus (MRSA) is currently regarded as one of the most important drug-resistant pathogens causing nosocomial and communityacquired infections. Although berberine (BER) has shown anti-MRSA activity, the underlying mechanism is still unclear. In this study, the damage caused by BER on the cell surface of MRSA was systematically investigated by performing BER susceptibility test, determining K + and alkaline phosphatase (ALP) release, detecting morphological alterations using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and ascertaining lipid profiles. The results showed that the minimum inhibitory concentration (MIC) of BER against MRSA252 was 128 µg/ml. Under the sub-MIC doses of BER, cell membrane permeability gradually increased in a dose-dependent manner, and 1 × MIC led to 43.8% higher K + leakage and fourfold higher ALP secretion. The injuries on MRSA cell surface were further verified by SEM and TEM, and some cells displayed a doughnut-shaped structure. BER significantly altered the fatty acid species contents, including saturated fatty acids (C 14:0 , C 15:0 , C 16:0 , C 18:0 , and C 20:0 ), and unsaturated fatty acids (C 20:4 , C 20:1 , and C 18:1 ), indicating that BER compromised cell membrane integrity via lipid fluctuation. Thus, the findings of this study could help to unravel the molecular mechanism of BER against MRSA.

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Antibacterial activity and mechanism of lactobionic acid against Staphylococcus aureus

Cited by 33 publications

References 33 publications

Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage

Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage

TMT-based proteomic analysis of the antibiotic effects of ShangKeHuangShui against Straphylococcus aureus

Berberine Damages the Cell Surface of Methicillin-Resistant Staphylococcus aureus

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