2008
DOI: 10.1128/aem.02001-07
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Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli

Abstract: The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Redu… Show more

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Cited by 1,671 publications
(926 citation statements)
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References 32 publications
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“…This will be lacking in case of bare-AgNPs, due to absence of the polyamine. Although reported earlier that Gram-positive bacteria shows greater resistance to silver nanoparticles (Feng et al 2000;Jung et al 2008), but antimicrobial efficacy of the newly synthesized put-AgNPs against all the four Gram-positive bacterial strains (B. cereus, B. subtilis, M. luteus and S. aureus) is noteworthy. Interestingly, the MICs of put-AgNPs against all bacterial strains were in their minimum values than compared to the bare-AgNPs.…”
Section: Minimum Inhibitory Concentrationmentioning
confidence: 99%
“…This will be lacking in case of bare-AgNPs, due to absence of the polyamine. Although reported earlier that Gram-positive bacteria shows greater resistance to silver nanoparticles (Feng et al 2000;Jung et al 2008), but antimicrobial efficacy of the newly synthesized put-AgNPs against all the four Gram-positive bacterial strains (B. cereus, B. subtilis, M. luteus and S. aureus) is noteworthy. Interestingly, the MICs of put-AgNPs against all bacterial strains were in their minimum values than compared to the bare-AgNPs.…”
Section: Minimum Inhibitory Concentrationmentioning
confidence: 99%
“…The teikoic acids are negatively charged (having -COOH or -COO-group and phosphate group), thus providing a negative charge on the surface of bacterial cells. Silver nanoparticles have a positive charge (Ag + ), making it easier to bind to the negatively charged surface of the bacteria (Kim et al, 2007;Feng et al, 2000;and Jung et al, 2008). The -COO-group will bind with Ag + .…”
Section: The Sem Photograph Of the Bacterial Cellulosementioning
confidence: 99%
“…Test cultures were then incubated for 24 h at 37 °C. Antimicrobial activity was evaluated using agar well diffusion method [20], plate count method [21] and contact method [22].…”
Section: Antimicrobial Assaymentioning
confidence: 99%