Antibacterial activity and mechanism of B-type oligomeric procyanidins from lotus seedpod on enterotoxigenic Escherichia coli

Tang, Cuie; Xie, Bijun; Sun, Zhida

doi:10.1016/j.jff.2017.09.046

Cited by 49 publications

(27 citation statements)

References 65 publications

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“…This effect mostly relies on antiadhesive action that prevents bacterial colonization of the urinary tract and has been described about A-type PAs but was not observed in the case of B-type structures [ 52 , 53 ]. Regarding efficacy against gastrointestinal E. coli infections, Tang et al [ 54 ] reported that B-type oligomeric procyanidins (extracted from lotus seedpod) could inhibit growth of two ETEC strains at 800 and 1200 µg/mL concentrations and showed bactericidal effect at 1500 µg/mL. Against the same bacteria, A-type oligomeric procyanidins (source: litchi pericarp) were only bacteriostatic at 2500 and 3500 µg/mL, while bactericidal at 3000 and 4500 µg/mL concentrations [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

et al. 2020

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Beneficial effects of flavonoids are widely known in human medicine, but less information is available about their veterinary usage. Based on their antioxidant and antibacterial activity, proanthocyanidins (PAs) and luteolin (LUT) might be used in the prevention and treatment of gastrointestinal infections in swine. In this study, in vitro beneficial effects of grape seed oligomeric proanthocyanidins (GSOPs) and LUT were investigated against bacterial endotoxin (LPS)-induced oxidative stress in IPEC-J2 porcine epithelial intestinal cells. Furthermore, antibacterial effects of GSOP and LUT were assessed against field isolates of Escherichia coli and Salmonella enterica ser. Typhimurium. Both GSOP and LUT were found to possess potent in vitro antioxidant activity in LPS-treated IPEC-J2 cells; furthermore, they showed a bacteriostatic effect against the tested bacterial strains of porcine origin. Both flavonoids seem to be effective in the protection of porcine intestinal epithelial cells against Gram-negative bacteria in vitro, but further in vivo studies are necessary to confirm these activities and to establish their optimal dosage regimen for future usage in veterinary practice.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

et al. 2020

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“…It is believed that the outer cell wall of Gram-negative bacteria contains lipoproteins and that the peptidoglycan layer is thinner than that of Gram-positive bacteria. However, not only do the cell walls of Gram-positive bacteria have a much thicker peptidoglycan layer, but also the presence of phosphoric acid causes Gram-positive bacteria to be more negatively charged than Gram-negative bacteria [ 28 , 29 ]. In yeast, polysaccharides find it difficult to bind to the polysaccharide receptors on the cell surface and to penetrate the cell wall due to the wall’s thickness and the deeply encapsulated membrane proteins by dextran [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure

Liu

Chen

et al. 2020

Marine Drugs

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Seaweed sulfated polysaccharides have attracted significant attention due to their antibacterial activity. This work investigated the antibacterial activity and mechanism of depolymerized sulfated galactans from Eucheuma serra (E. serra) and Gracilaria verrucosa (G. verrucosa) against enterotoxigenic Escherichia coli (ETEC) K88. The results show that removing the metal ions improves the anti-ETEC K88 activity of the galactans. The fluorescence labeling study confirmed that the sulfated galactans penetrated the cell walls and eventually reached the interior of the ETEC K88. Nucleic acid staining and intracellular protein leakage were also observed, indicating the destruction of permeability and integrity of the cell membrane. Interestingly, the two polysaccharides exhibited no effect on the proliferation of the selected Gram-positive bacteria and yeast. This indicates that the cell wall structure of the microorganisms could influence the bacteriostatic activity of the sulfated polysaccharides, as well. These results suggest that the sulfated seaweed polysaccharides might have potential application value in antibacterial diarrhea.

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“…e intracellular potassium leakage of the supernatant was determined using flame atomic absorption spectrophotometry (AAS) (AAnalyst 100, PerkinElmer Co., USA) as previously described by Tang et al [18]. A linear relationship between potassium concentration and emission was obtained using potassium standards (analytical grade, Sigma-Aldrich, Poole, United Kingdom).…”

Section: Measurement Of the Intracellular Protein Leakage Andmentioning

confidence: 99%

Efficacy and Mechanism of Ultrasound Combined with Slightly Acidic Electrolyzed Water for Inactivating Escherichia coli

Guo

Zhang

et al. 2021

Journal of Food Quality

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In the present study, the synergetic effect and mechanism of ultrasound (US) and slightly acidic electrolyzed water (SAEW) on the inactivation of Escherichia coli (E. coli) were evaluated. The results showed that US combined with SAEW treatment showed higher sanitizing efficacy for reducing E. coli than US and SAEW alone treatment. US and US combined with SAEW treatments resulted in smaller particle size of E. coli compared to the control and SAEW treatment. In addition, US combined with SAEW treatment induced the highest potassium leakage. However, the highest protein leakage was recorded in US treatment. Moreover, scanning and transmission electron microscopy analysis revealed that the greatest damage of the appearance and ultrastructure of E. coli was achieved after US combined with SAEW treatment. The synergetic effect was also confirmed by CLSM analysis. Fluorescence spectroscopy suggested that treatments of US, SAEW, and US combined with SAEW changed protein conformation of E. coli. Overall, the present study demonstrated that the sterilization mechanism of US combined with SAEW treatment was decreasing the particle size and disrupting the permeability of cell membrane and the cytoplasmic ultrastructure as well as changing protein conformation of E. coli.

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Antibacterial activity and mechanism of B-type oligomeric procyanidins from lotus seedpod on enterotoxigenic Escherichia coli

Cited by 49 publications

References 65 publications

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure

Efficacy and Mechanism of Ultrasound Combined with Slightly Acidic Electrolyzed Water for Inactivating Escherichia coli

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