Antibacterial activity and mechanisms of depolymerized fucoidans isolated from Laminaria japonica

Liu, Ming; Liu, Yixiang; Cao, Min‐Jie; Liu, Guangming; Chen, Qingchou; Sun, Le-Chang; Chen, Haixiu

doi:10.1016/j.carbpol.2017.05.060

Cited by 172 publications

(111 citation statements)

References 45 publications

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“…According to ours and other previous studies, sulfate groups of marine polysaccharides are closely related with their biological activity, such as antibacterial and anti-tumor activities [3,6]. The content of sulfate groups should be another important factor for the inhibiting effect of sulfated galactans on ETEC K88.…”

Section: Effect Of Molecular Weight On Antibacterial Activitysupporting

confidence: 51%

“…The peak at 933 cm −1 can be attributed to 3,6-AG (C-O-S) [16]. The most important peaks for ESP and GSP and their depolymerized products are located at approximately 1244 and 1265 cm −1 , respectively, which correspond to the stretching vibration of the ester sulfate groups (S=O) [6,17]. Considering the stronger sulfated group peak in ESP spectra than that in GSP, the former was more sulfated than the latter.…”

Section: Effect Of Depolymerization On the Antibacterial Activity Of mentioning

confidence: 97%

“…Attributing to the residues, including tryptophan, tyrosine, and phenylalanine, proteins usually can exhibit fluorescence property [6]. Therefore, if the membrane proteins of bacteria were combined with sulfated polysaccharides, their fluorescence properties should be suppressed [6]. The changes of membrane proteins were investigated to reveal the antibacterial mechanisms of sulfated galactans against ETEC-K88.…”

Section: Effect Of Sulfated Galactans On Cell Membrane Proteinsmentioning

confidence: 99%

“…As the main bioactive components in marine algae, sulfated polysaccharides, particularly their bacteriostatic or bactericidal activity, have attracted attention [4]. In brown algae, the sulfated polysaccharides from Laminaria japonica and Sargassum swartzii can inhibit Gram-negative and -positive bacteria including E. coli, Vibrio cholera, Salmonella typhi, and Staphylococcus aureus [5,6]. The sulfated polysaccharides from green algae (Chaetomorpha aerea) and microalgae (Porphyridium cruentum) also display inhibitory effect on S. aureus and Salmonella enteritidis, respectively [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Liu

et al. 2019

Marine Drugs

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Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88. A structure–activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.

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Section: Effect Of Molecular Weight On Antibacterial Activitysupporting

confidence: 51%

Section: Effect Of Depolymerization On the Antibacterial Activity Of mentioning

confidence: 97%

Section: Effect Of Sulfated Galactans On Cell Membrane Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Liu

et al. 2019

Marine Drugs

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“…The structure of fucoidan and its composition strongly depend on the extraction source (the type of algae) and the method of extraction, thereby affecting its effectiveness. Fucoidan extracted from different species has varied biological activities, including anti-coagulant [19], antioxidant [20], immunomodulatory [21], anti-inflammatory [22], antibacterial activity [23], and anti-obesity [24,25] properties. Alternatively, different processing methods are also important factors that influence the molecular conformational changes.…”

Section: Introductionmentioning

confidence: 99%

Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression

Chen

Huang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic effects. We aimed to study the effect of fucoidan on the growth of ULs activated by transforming growth factor beta (TGFβ). Methods: We used ELT-3 (Eker rat leiomyoma tumor-derived cells) and HUtSMC (human uterine smooth muscle cells) as in vitro models. Cell viability was determined by the MTT assay. Cell colony formation was stained using crystal violet. The side population, cell cycle and apoptosis were analyzed using flow cytometry. Protein expression was assayed by western blot analysis. We also conducted in vivo experiments to confirm the inhibitory effects of fucoidan in nude mouse xenograft models. Tumor tissues were assayed by immunohistochemistry analysis. Results: In our study, fucoidan caused a 50% growth inhibition using a dose of 0.5 mg/ml and decreased the stem cell activity after 48 h. In addition, fucoidan induced sub-G1 cell cycle arrest and apoptosis. Fucoidan down-regulated fibronectin, vimentin, α-SMA and the COL1A1 protein levels in TGFβ3-induced ELT-3 cells. In the cellular mechanism, fucoidan abrogated TGFβ3-induced levels of p-Smad2 and p-ERK1/2, as well as β-catenin translocation into the nucleus. Furthermore, fucoidan suppressed xenograft tumor growth in vivo. Conclusion: Fucoidan displays anti-proliferation and anti-fibrotic effects and exerts protective effects against ULs development.

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Algal Polysaccharides and Their Biological Properties

Cheong

Jesumani

Khan

et al. 2021

Recent Advances in Micro and Macroalgal Processing

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Antibacterial activity and mechanisms of depolymerized fucoidans isolated from Laminaria japonica

Cited by 172 publications

References 45 publications

Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression

Algal Polysaccharides and Their Biological Properties

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