Antibacterial activity and mode of action of potassium tetraborate tetrahydrate against soft-rot bacterial plant pathogens

Liu, Yingyu; Filiatrault, Melanie J.

doi:10.1099/mic.0.000948

Cited by 4 publications

(10 citation statements)

References 46 publications

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“…The gene was known for controlling phytopathogens in the tomato plant in this study. Our research coincides with the report of Liu & Filiatrault (2020) that reveal how the gene promotes the antibacterial activity of potassium tetraborate tetrahydrate (PTB) on Pectobacterium atrosepticum SCRI1043 wild-type that causes soft-rot bacterial phytopathogens. Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18) ( nadD ) gene is abundant in HR produced by microbial communities inhabiting the rhizosphere and carries out the function of defense against phytopathogens.…”

Section: Discussionsupporting

confidence: 89%

Unraveling the functional genes present in rhizosphere microbiomes of Solanum lycopersicum

Adedayo¹,

Fadiji²,

Babalola³

2023

PeerJ

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The microbiomes living in the rhizosphere soil of the tomato plant contribute immensely to the state of health of the tomato plant alongside improving sustainable agriculture. With the aid of shotgun metagenomics sequencing, we characterized the putative functional genes (plant-growth-promoting and disease-resistant genes) produced by the microbial communities dwelling in the rhizosphere soil of healthy and powdery mildew-diseased tomato plants. The results identified twenty-one (21) plant growth promotion (PGP) genes in the microbiomes inhabiting the healthy rhizosphere (HR) which are more predomiant as compared to diseased rhizosphere (DR) that has nine (9) genes and four (4) genes in bulk soil (BR). Likewise, we identified some disease-resistant genes which include nucleotide binding genes and antimicrobial genes. Our study revealed fifteen (15) genes in HR which made it greater in comparison to DR that has three (3) genes and three (3) genes in bulk soil. Further studies should be conducted by isolating these microorganisms and introduce them to field experiments for cultivation of tomatoes.

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Section: Discussionsupporting

confidence: 89%

Unraveling the functional genes present in rhizosphere microbiomes of Solanum lycopersicum

Adedayo¹,

Fadiji²,

Babalola³

2023

PeerJ

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“…does the agricultural industry seek at inhibiting growth of Dickeya. An interesting double-ring-of-inhibition phenotype was observed with Dickeya in diffusion assays using agar plates (Liu & Filiatrault, 2020). This phenotype has not been previously seen in Dickeya or other bacteria.…”

Section: Introductionsupporting

confidence: 49%

“…Disk diffusion assays were used to test the antimicrobial activity of PTB on the four strains of Dickeya and to confirm their double-ring phenotype (Liu & Filiatrault, 2020). Triplicate overnight cultures were grown in LB, adjusted to 2×10 8 c.f.u.…”

Section: Antibacterial Activity Of Ptbmentioning

confidence: 99%

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Investigation of Potassium Tetraborate Resistance in Dickeya spp.

Lou

2023

CURJ

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Dickeya spp. are common plant pathogens associated with bacterial soft rot, potato blackleg, and slow wilt, which are plant diseases that account for major losses in the agricultural industry. The diseases caused by these bacterial species are not yet fully managed with existing techniques, and new approaches need to be considered to minimize future crop loss. Previous research has shown that the inorganic salt potassium tetraborate tetrahydrate (PTB) can inhibit the growth of Dickeya species; however, disk diffusion assays result in a unique phenotype with two zones of inhibition. This study investigates the effects of PTB on the growth of four Dickeya spp.. It was hypothesized that the production of phage is responsible for the two zones of inhibition. Disk diffusion assays and growth curves were used to confirm the impact of PTB on Dickeya and attempts were made to directly isolate phage from the strains. To elucidate the mechanism of action of PTB, Tn-Seq libraries were used to determine which genes are required for growth in the presence of PTB. Tn-Seq libraries showed that different Dickeya strains shared seven overlapping genes including stress-related genes that increase bacterial resistance to PTB. Gene expression studies were used to determine the changes in gene expression that result from PTB exposure. Preliminary results showed that exposure to PTB induces the expression of stress-related genes in Dickeya to increase survival in the presence of the compound. Further research is needed to better understand the implications of observed changes in bacterial gene expression.

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“…In contrast to all these studies, no evidence was obtained for pro-virulence of the cpdB gene of Yersinia enterocolítica in an oral and intravenous mouse infection model [ 4 ]. The cpdB gene of the plant pathogen Pectobacterium atrosepticum offers a different point of view, since gene mutations make the pathogen resistant to potassium tetraborate tetrahydrate used as disinfectant [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic Distribution of Pro-Virulent cpdB-like Genes in Eubacteria and Comparison of the Enzyme Specificity of CpdB-like Proteins from Salmonella enterica, Escherichia coli and Streptococcus suis

Ribeiro

Canales

Costas

et al. 2023

IJMS

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The cpdB gene is pro-virulent in avian pathogenic Escherichia coli and in Salmonella enterica, where it encodes a periplasmic protein named CpdB. It is structurally related to cell wall-anchored proteins, CdnP and SntA, encoded by the also pro-virulent cdnP and sntA genes of Streptococcus agalactiae and Streptococcus suis, respectively. CdnP and SntA effects are due to extrabacterial hydrolysis of cyclic-di-AMP, and to complement action interference. The mechanism of CpdB pro-virulence is unknown, although the protein from non-pathogenic E. coli hydrolyzes cyclic dinucleotides. Considering that the pro-virulence of streptococcal CpdB-like proteins is mediated by c-di-AMP hydrolysis, S. enterica CpdB activity was tested as a phosphohydrolase of 3′-nucleotides, 2′,3′-cyclic mononucleotides, linear and cyclic dinucleotides, and cyclic tetra- and hexanucleotides. The results help to understand cpdB pro-virulence in S. enterica and are compared with E. coli CpdB and S. suis SntA, including the activity of the latter on cyclic-tetra- and hexanucleotides reported here for the first time. On the other hand, since CpdB-like proteins are relevant to host-pathogen interactions, the presence of cpdB-like genes was probed in eubacterial taxa by TblastN analysis. The non-homogeneous genomic distribution revealed taxa with cpdB-like genes present or absent, identifying eubacteria and plasmids where they can be relevant.

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Antibacterial activity and mode of action of potassium tetraborate tetrahydrate against soft-rot bacterial plant pathogens

Abstract: Bacterial soft rot caused by the bacteria Dickeya and Show more

Cited by 4 publications

References 46 publications

Unraveling the functional genes present in rhizosphere microbiomes of Solanum lycopersicum

Unraveling the functional genes present in rhizosphere microbiomes of Solanum lycopersicum

Investigation of Potassium Tetraborate Resistance in Dickeya spp.

Genomic Distribution of Pro-Virulent cpdB-like Genes in Eubacteria and Comparison of the Enzyme Specificity of CpdB-like Proteins from Salmonella enterica, Escherichia coli and Streptococcus suis

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