Antibacterial Activity and Synergistic Effect of Biosynthesized AgNPs with Antibiotics Against Multidrug-Resistant Biofilm-Forming Coagulase-Negative Staphylococci Isolated from Clinical Samples

Thomas, Roshmi; Nair, Aswathi P.; Kr, Soumya; Mathew, Jyothis; Ek, Radhakrishnan

doi:10.1007/s12010-014-0852-z

Cited by 69 publications

(32 citation statements)

References 33 publications

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“…The efficacy of the formulated nano-imipenem on microbial adhesion and biofilm formation was evaluated using microtitre plate method [26]. Growth medium containing 1/4 MICs of IMP, IMP/PCL and IMP/PLGA were prepared and 100 μl of each preparation was distributed into wells in triplicates.…”

Section: Methodsmentioning

confidence: 99%

Imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates

2017

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BackgroundCarbapenem-resistance is an extremely growing medical threat in antibacterial therapy as the incurable resistant strains easily develop a multi-resistance action to other potent antimicrobial agents. Nonetheless, the protective delivery of current antibiotics using nano-carriers opens a tremendous approach in the antimicrobial therapy, allowing the nano-formulated antibiotics to beat these health threat pathogens. Herein, we encapsulated imipenem into biodegradable polymeric nanoparticles to destroy the imipenem-resistant bacteria and overcome the microbial adhesion and dissemination. Imipenem loaded poly Ɛ-caprolactone (PCL) and polylactide-co-glycolide (PLGA) nanocapsules were formulated using double emulsion evaporation method. The obtained nanocapsules were characterized for mean particle diameter, morphology, loading efficiency, and in vitro release. The in vitro antimicrobial and anti adhesion activities were evaluated against selected imipenem-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates.ResultsThe obtained results reveal that imipenem loaded PCL nano-formulation enhances the microbial susceptibility and antimicrobial activity of imipenem. The imipenem loaded PCL nanoparticles caused faster microbial killing within 2–3 h compared to the imipenem loaded PLGA and free drug. Successfully, PCL nanocapsules were able to protect imipenem from enzymatic degradation by resistant isolates and prevent the emergence of the resistant colonies, as it lowered the mutation prevention concentration of free imipenem by twofolds. Moreover, the imipenem loaded PCL eliminated bacterial attachment and the biofilm assembly of P. aeruginosa and K. pneumoniae planktonic bacteria by 74 and 78.4%, respectively.ConclusionsThese promising results indicate that polymeric nanoparticles recover the efficacy of imipenem and can be considered as a new paradigm shift against multidrug-resistant isolates in treating severe bacterial infections.

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Section: Methodsmentioning

confidence: 99%

Imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates

2017

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“…The biological methods for AgNPs synthesis using bacteria, fungi, proteins, polypeptides, nucleic acids and plant extracts are simple, nontoxic, affordable and environmentally friendly. These biological methods can be used to generate nanoparticles with acceptable size and morphology [28,29] .…”

Section: Research Papermentioning

confidence: 99%

Green Synthesis and Characterization of Silver Nanoparticles from Crocus Mathewii; A Disremembered Turkish Flowering Plant

Yildiztekin¹,

Nadeem²,

Yildiztekin³

et al. 2017

pharmaceutical-sciences

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Mahmut, et al.: Characterization of Silver Nanoparticles from Crocus mathewiiThis study reports the first ever chemical study on Crocus mathewii, a Turkish endemic and forgotten flowering plant. Silver nanoparticles were green synthesized from one mmol×l -1 silver nitrate extract of C. mathewii. The water-suspended silver nanoparticles showed a peak at 423 nm in UV/Vis spectrophotometer that suggested smaller particle size due to its less surface plasmon resonance. Transmission electron microscopy analysis also proved excellent polydispersity of quasi-spherical particle size distribution with average particles of 7.5 nm (2.5-25 nm). The presence of silver in the nanoparticles was analyzed by X-ray diffraction, while Fourier transform infrared spectroscopy analysis showed the presence of various -OH and -NH containing organics. The results proved that the crude extract of C. mathewii can be used for green synthesis of silver nanoparticles in future for medicinal uses.

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“…Thus in the current study, PCL was selected as the candidate polymer for the development of electrospun membrane with persistent antimicrobial delivering effect of incorporated silver nanoparticles (AgNPs) against pathogens, which cause wound-related and device-centered infection. During last few years of our study, we have already reported the excellent effect of biosynthesized AgNPs on coagulase-negative staphylococci (CoNS) isolated from clinical samples [14]. To bring this to an application level, the current work was designed by imparting antimicrobial effect of AgNPs into electrospun PCL nanocomposite.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

Thomas

Soumya

Mathew

et al. 2015

Appl Biochem Biotechnol

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Biosynthesized silver nanoparticles (AgNPs) incorporated polycaprolactone (PCL) nanomembrane was prepared by electrospinning as a cost-effective nanocomposite for application as an antimicrobial agent against wound infection. The nanocomposite membrane was characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis and Scanning Electron microscopy (SEM). The hydrophilicity analysis of electrospun membranes as evaluated by water contact angle measurement showed the change of hydrophobicity of PCL to hydrophilic upon incorporation of silver nanoparticles. Better mechanical properties were also observed for PCL membrane due to the incorporation of silver nanoparticles and are highly supportive to explore its biomedical applications. Further antibacterial analysis of silver nanoparticle-incorporated PCL membrane against common wound pathogens coagulase-negative Staphylococcus epidermidis and Staphylococcus haemolyticus showed remarkable activity. As biosynthesized AgNPs are least explored for clinical applications, the current study is a promising cost-effective method to explore the development of silver nanoparticle-based electrospun nanocomposite to resist wound-associated infection.

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Antibacterial Activity and Synergistic Effect of Biosynthesized AgNPs with Antibiotics Against Multidrug-Resistant Biofilm-Forming Coagulase-Negative Staphylococci Isolated from Clinical Samples

Cited by 69 publications

References 33 publications

Imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates

Imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates

Green Synthesis and Characterization of Silver Nanoparticles from Crocus Mathewii; A Disremembered Turkish Flowering Plant

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

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