Antibacterial activity and wound healing potential of Cycas thouarsii R.Br n-butanol fraction in diabetic rats supported with phytochemical profiling

Binsuwaidan, Reem; Elekhnawy, Engy; Elseady, Walaa S.; Keshk, Walaa Arafa; Shoeib, Nagwa A.; Attallah, Nashwah G. M.; Mokhtar, Fatma Alzahraa; Hadi, Soha R. Abd El; Ahmed, Eman Ibrahim; Magdeldin, Sameh; Negm, Walaa A.

doi:10.1016/j.biopha.2022.113763

Cited by 17 publications

(7 citation statements)

References 35 publications

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“…In addition to the above studies, a correlation between wound contraction and MMP-9, [108][109][110][111][112][113][114][115][116]56] and MMP-2 [113,117] levels was observed in other studies. This means wounds that healed or recovered more quickly had a lower extent of these proteases (or their genes or their activity) than wounds that healed to a lesser extent (of course, in many cases, normalized to the protein content).…”

Section: Protease Level In the Uninjured Tissue And Healing Coursementioning

confidence: 77%

“…Daily topical application of Cycas thouarsii R.Br n‐butanol fraction (a herbal extract) in a gel formulation in diabetic rats’ wounds for 30 days led to a lower MMP‐9 level compared to groups treated with vehicle or left untreated. [ 115 ] Saleh and colleagues investigated the effect of Indigo leaf extract on the wound healing of rats. [ 227 ] They applied the extract over the wounds and measured the expression of MMP‐2 and ‐9 in the skin tissue.…”

Section: Controlling the Protease Expression And Activitymentioning

confidence: 99%

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Approaches to Control and Monitor Protease Levels in Chronic Wounds

Kolahreez,

Ghasemi‐Mobarakeh,

Liebner

et al. 2024

Advanced Therapeutics

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Some wounds do not follow the typical healing trajectory and show very little, if any, progress. They dreadfully threaten the patients' quality of life and may even lead to limb amputation and death. Several interrelated systemic and local factors may cause a delay in wound healing. The microenvironment of these wounds could be different from wounds that heal spontaneously or with standard treatment. As discussed in the present work, proteases’ levels or activity could be elevated in some cases of chronic ulcers; infection could aggravate the situation. A detailed overview of the proteases’ effect on wound healing is presented in this paper. Assuming untimely or irregularly excessive proteolytic activity plays a pathological role, some researchers targeted attenuation of protease level as a strategy to promote healing. This could be done via different approaches, including physical removal, inhibiting their activity, and controlling their expression directly through gene silencing or indirectly, for instance, by resolving infection. Apart from targeting protease modulation as a therapeutic strategy, many studies have examined the protease state to gain insights into the underlying mechanisms by which a remedy, dressing, or specific therapy has affected the healing process. This article aims to provide an overview of the studies in these two categories. Of course, various proteases are involved in the healing process, but in each study, usually, only one or some of them were investigated; matrix metalloproteinase‐2 (MMP‐2), MMP‐9, and human neutrophil elastase (HNE) are among them. Furthermore, in some cases, cumulative proteolytic activity has been evaluated in terms of gelatinolytic, collagenolytic, or caseinolytic activity, which is also absolutely beneficial. Some research groups have considered the potential of proteases as a diagnostic or prognostic biomarker and have tried to develop some sensors or indicators; this topic has also been covered in the present work.This article is protected by copyright. All rights reserved

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Section: Protease Level In the Uninjured Tissue And Healing Coursementioning

confidence: 77%

Section: Controlling the Protease Expression And Activitymentioning

confidence: 99%

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Kolahreez,

Ghasemi‐Mobarakeh,

Liebner

et al. 2024

Advanced Therapeutics

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“…Figure 4 depicts fragmentation reactions of chlorogenic acid. One might argue that its MS fragmentation processes have been extensively examined [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52]. Thus, we only sketch out MS analysis of CA showing the intensity ratios of the peaks at m/z 191 and 179, thus quantitatively distinguishing not only among its positional 1-O-, 3-O-, 4-O-, and 5-Oisomers, but also between transand cis-isomers of the product 3-(3,4-dihydroxy-phenyl)acrylic acid anions [35].…”

Section: Mass Spectrometric Data On Chlorogenic Acidmentioning

confidence: 99%

Mass Spectrometric Identification of Metabolites after Magnetic-Pulse Treatment of Infected Pyrus communis L. Microplants

Upadyshev,

Ivanova,

Motyleva

2023

IJMS

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The major goal of this study is to create a venue for further work on the effect of pulsed magnetic fields on plant metabolism. It deals with metabolite synthesis in the aforementioned conditions in microplants of Pyrus communis L. So far, there have been glimpses into the governing factors of plant biochemistry in vivo, and low-frequency pulsed magnestatic fields have been shown to induce additional electric currents in plant tissues, thus perturbing the value of cell membrane potential and causing the biosynthesis of new metabolites. In this study, sixty-seven metabolites synthesized in microplants within 3–72 h after treatment were identified and annotated. In total, thirty-one metabolites were produced. Magnetic-pulse treatment caused an 8.75-fold increase in the concentration of chlorogenic acid (RT = 8.33 ± 0.0197 min) in tissues and the perturbation of phenolic composition. Aucubin, which has antiviral and antistress biological activity, was identified as well. This study sheds light on the effect of magnetic fields on the biochemistry of low-molecular-weight metabolites of pear plants in vitro, thus providing in-depth metabolite analysis under optimized synthetic conditions. This study utilized high-resolution gas chromatography-mass spectrometry, metabolomics methods, stochastic dynamics mass spectrometry, quantum chemistry, and chemometrics, respectively. Stochastic dynamics uses the relationships between measurands and molecular structures of silylated carbohydrates, showing virtually identical mass spectra and comparable chemometrics parameters.

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“… Diabetic nephropathy Amelioration in endothelial dysfunction, fatty liver disease, diabetes mellitus, acute kidney injury, fibrosis, hypertensive disease, obesity, etc. [ 34 ] 4 - Quercetin and kaempferol TNF, JUN, IL6, STAT3, MAPK1, and MAPK3 Acne Vulgaris Anti-inflammatory effect and regulate the excessive lipogenesis in sebaceous glands via different signaling pathways [ 104 ] 5 Gelsemium elegans Gelegamine E, gelsesyringalidine, humantenine, gelsedine, 19-z-akuammidine, gelegamine B, tabersonine, koumine, gelsemine MAPK3, HSP90AA1, JUN, EGFR, CDK1, TNF, CCND1, ESR1, PRKACA, CCNA2, CDC25C, CDK2, CCNB1, AR, CREBBP, AURKA, CDC25A, CHEK1, BCL2L1, and PIK3CD Anti-cancer Pathways in cancer, cell cycle, and colorectal cancer [ 105 ] 6 Danning Tablets (A polyherbal formulation) Quercetin, ß-sitosterol, luteolin, kaempferol, supraene, curcumenolactone C, and stigmasterol IL6, MAPK8, VEGFA, CASP3, ALB, APP, MYC, PPARG, and RELA Nonalcoholic Fatty Liver Disease Lipid metabolism, inflammation, oxidation, insulin resistance (IR), atherosclerosis, and apoptosis [ 106 ] 7 Panax notoginseng Mandenol, beta-sitosterol, stigmasterol, ginsenoside rh2, ginsenoside f2, quercetin VEGFA, MMP-9, MMP-2, FGF2, and COX-2 Diabetic Retinopathy Intervention of angiogenesis, inflammation, and apoptosis [ 107 ] <...…”

Section: Review Findingsmentioning

confidence: 99%

Metabolomics and Network Pharmacology in the Exploration of the Multi-Targeted Therapeutic Approach of Traditional Medicinal Plants

Sharma

Yadav

2022

Plants

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Metabolomic is generally characterized as a comprehensive and the most copious analytical technique for the identification of targeted and untargeted metabolite diversity in a biological system. Recently, it has exponentially been used for phytochemical analysis and variability among plant metabolites, followed by chemometric analysis. Network pharmacology analysis is a computational technique used for the determination of multi-mechanistic and therapeutic evaluation of chemicals via interaction with the genomes involved in targeted or untargeted diseases. In considering the facts, the present review aims to explore the role of metabolomics and network pharmacology in the scientific validation of therapeutic claims as well as to evaluate the multi-targeted therapeutic approach of traditional Indian medicinal plants. The data was collected from different electronic scientific databases such as Google Scholar, Science Direct, ACS publication, PubMed, Springer, etc., using different keywords such as metabolomics, techniques used in metabolomics, chemometric analysis, a bioinformatic tool for drug discovery and development, network pharmacology, methodology and its role in biological evaluation of chemicals, etc. The screened articles were gathered and evaluated by different experts for their exclusion and inclusion in the final draft of the manuscript. The review findings suggest that metabolomics is one of the recent most precious and effective techniques for metabolite identification in the plant matrix. Various chemometric techniques are copiously used for metabolites discrimination analysis hence validating the unique characteristic of herbal medicines and their derived products concerning their authenticity. Network pharmacology remains the only option for the unique and effective analysis of hundreds of chemicals or metabolites via genomic interaction and thus validating the multi-mechanistic and therapeutic approach to explore the pharmacological aspects of herbal medicines for the management of the disease.

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Antibacterial activity and wound healing potential of Cycas thouarsii R.Br n-butanol fraction in diabetic rats supported with phytochemical profiling

Cited by 17 publications

References 35 publications

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Mass Spectrometric Identification of Metabolites after Magnetic-Pulse Treatment of Infected Pyrus communis L. Microplants

Metabolomics and Network Pharmacology in the Exploration of the Multi-Targeted Therapeutic Approach of Traditional Medicinal Plants

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