Antibacterial activity of 3,3′-dihydroxycurcumin (DHC) is associated with membrane perturbation

Polaquini, Carlos Roberto; Morão, Luana Galvão; Nazaré, Ana Carolina; Torrezan, Guilherme Silva; Dilarri, Guilherme; Cavalca, L.; Campos, Denise; Silva, Isabel Cristiane da; Pereira, Jesse Augusto; Scheffers, Dirk‐Jan; Duque, Cristiane; Pavan, Fernando Rogério; Ferreira, Henrique; Regasini, Luis Octávio

doi:10.1016/j.bioorg.2019.103031

Cited by 17 publications

(19 citation statements)

References 61 publications

(68 reference statements)

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“…Other authors have also reported a high sensitivity of these pathogens to curcumin, although the above-mentioned species have been examined to varying degrees. A lot of data exists for S. aureus (e.g., [ 20 , 32 , 34 , 35 , 37 , 54 ]), while in the case of S. pyogenes [ 20 , 31 , 33 , 55 ], and A. lwoffii [ 20 ], they are few. The very strong antimicrobial activity of curcumin, probably due to the use of acetone as a solvent, was shown in the studies of Lutomski et al [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, the activity of curcumin against S. haemolyticus has not yet been studied, whereas the previous results for E. faecalis were similar to our findings. The MIC of reference strains of this species varied between 156 [ 37 ] and 625 µg/mL [ 39 , 40 ] for E. faecalis ATCC 51299 and E. faecalis ATCC 29212, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…In turn, this activity of curcumin against such bacteria as A. lwoffii [ 20 ], Proteus mirabilis [ 24 , 30 ], Serratia marcescens [ 20 , 24 ], Stenotrophomonas maltophilia [ 31 ], and Streptococcus agalactiae [ 30 ] has been examined sporadically. In the contemporary research, the in vitro ability of curcumin to inhibit microbial growth has frequently been tested against a small (4–6) number of species representing a small group of taxa, mostly E. coli , P. aeruginosa , and S. aureus , and less often Bacillus subtilis and Enterococcus faecalis (e.g., [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ]). Some works have reported the minimum inhibitory concentration (MIC) value for only one species and a single, usually reference, strain (e.g., [ 39 , 40 , 41 , 42 ]).…”

Section: Introductionmentioning

confidence: 99%

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Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

2020

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Curcumin, a principal bioactive substance of turmeric (Curcuma longa L.), is reported as a strong antioxidant, anti-inflammatory, antibacterial, antifungal, and antiviral agent. However, its antimicrobial properties require further detailed investigations into clinical and multidrug-resistant (MDR) isolates. In this work, we tested curcumin’s efficacy against over 100 strains of pathogens belonging to 19 species. This activity was determined by the broth microdilution method and by calculating the minimum inhibitory concentration (MIC). Our findings confirmed a much greater sensitivity of Gram-positive than Gram-negative bacteria. This study exhibited a significantly larger variation in the curcumin activity than previous works and suggested that numerous clinical strains of widespread pathogens have a poor sensitivity to curcumin. Similarly, the MICs of the MDR types of Staphylococcus aureus, S. haemolyticus, Escherichia coli, and Proteus mirabilis were high (≥2000 µg/mL). However, curcumin was effective against some species and strains: Streptococcus pyogenes (median MIC = 31.25 µg/mL), methicillin-sensitive S. aureus (250 µg/mL), Acinetobacter lwoffii (250 µg/mL), and individual strains of Enterococcus faecalis and Pseudomonas aeruginosa (62.5 µg/mL). The sensitivity of species was not associated with its affiliation to the genus, and it could differ a lot (e.g., S. pyogenes, S. agalactiae and A. lwoffii, A. baumannii). Hence, curcumin can be considered as a promising antibacterial agent, but with a very selective activity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

2020

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“…So far, phytochemicals have remained the leading source for potential antimicrobial agents, whether investigated independently or in a combination with another substance [2,25]. Recent studies have demonstrated a wide range of antimicrobial properties of curcumin mediated by different mechanisms, such as alteration of gene expression, inhibition of bacterial DNA replication and disruption of the bacterial cell membrane [26][27][28].Literature data demonstrate the beneficial and preventive role of curcumin against various microorganisms [29][30][31]. Despite various studies evaluating the antimicrobial activity of curcumin, further research is required with different concentrations against different strains of microorganisms.…”

Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

Curcumin: Natural Antimicrobial and Anti Inflammatory Agent

Pehlivanović

Čaklovica

Lagumdžija

et al. 2021

JPRI

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The pursuance of novel antimicrobial and anti-inflammatory agents has been expanding due to a significant need for more efficient pharmacotherapy of various infections and chronic diseases. During the last decade, pharmacokinetics, pharmacodynamics and pharmacological properties of curcumin have been extensively studied. The aim of the present study was to evaluate the antibacterial activity of curcumin against both Gram-positive and Gram-negative bacteria as well as its antifungal activity by using in vitro agar well diffusion assay. Moreover, the anti-inflammatory activity of curcumin was determined with in vitro assay of inhibition of protein denaturation. Results demonstrated wide antimicrobial activity of curcumin upon all of the test bacteria and fungi. The strongest activity of curcumin was observed at a concentration of 0.50 mg/ml against S. aureus, L. monocytogenes, E. coli, P. aeruginosa and C. albicans, resulting in a maximum zone of inhibition of 14.7 mm, 14.3 mm, 13.7 mm, 10.7 mm and 10.7 mm, respectively. Findings suggested that the antimicrobial activity of curcuminis dependent upon the concentrations. Furthermore, results demonstrated high effectiveness of curcumin compared to standard acetylsalicylic acid in inhibiting heat-induced protein denaturation, which activity is also depended upon the concentrations. The present study emphasises the potential application of curcumin as a natural antimicrobial and anti-inflammatory agent. However, findings of this study are restricted to in vitro assays and consideration should be given to conducting a study involving wider dose range test substances as well as including further research on in vivo models.

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“…In our continuing search for new antibacterial phenolic compounds, we have synthesized and evaluated hundreds of compounds using antibacterial phenotypic assays against gram-positive and gram-negative species [ 13 , 14 , 15 , 16 ]. The structural design of our library of curcumin analogs has been based on phenolic functionality, because most of bacterial division inhibitors display at least one phenol ring [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A

et al. 2020

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Xanthomonas citri subsp. citri (X. citri) is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against X. citri and the model organism Bacillus subtilis. BC1 and T9A prevented growth of X. citri and B. subtilis in concentrations varying from 20 µg/mL to 40 µg/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in X. citri and B. subtilis. Both compounds mildly affected respiratory activity in X. citri, but T9A strongly decreased respiratory activity in B. subtilis. In line with that finding, intracellular ATP decreased strongly in B. subtilis upon T9A treatment, whereas BC1 increased intracellular ATP. In X. citri, both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in X. citri, and, although in B. subtilis the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action.

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Antibacterial activity of 3,3′-dihydroxycurcumin (DHC) is associated with membrane perturbation

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Cited by 17 publications

References 61 publications

Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

Curcumin: Natural Antimicrobial and Anti Inflammatory Agent

Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A

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