Antibacterial activity of alkaloids produced by endophytic fungus<i>Aspergillus</i>sp. EJC08 isolated from medical plant<i>Bauhinia guianensis</i>

Pinheiro, Eduardo Antonio Abreu; Carvalho, Josiwander Miranda; Santos, Diellem Cristina P. dos; Feitosa, André de Oliveira; Marinho, Patrícia Santana Barbosa; Guilhon, Giselle Maria Skelding Pinheiro; Souza, Afonso Duarte Leão de; Silva, Felipe M. A. da; Marinho, Andrey Moacir do Rosário

doi:10.1080/14786419.2012.750316

Cited by 89 publications

(57 citation statements)

References 13 publications

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“…B 371: 20160023 BGC 6 (Fumigaclavine). Fumigaclavines are bioactive alkaloids with numerous bioactivities ranging from antibacterial [52] to vasorelaxant [53]. Fumigaclavines are considered mycotoxins and purified compounds have a toxic impact on mammalian cells [54].…”

Section: Results and Discussion (A) Twenty-six Clustersmentioning

confidence: 99%

Secondary metabolite arsenal of an opportunistic pathogenic fungus

Bignell

Cairns²,

Throckmorton

et al. 2016

Phil. Trans. R. Soc. B

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Section: Results and Discussion (A) Twenty-six Clustersmentioning

confidence: 99%

Secondary metabolite arsenal of an opportunistic pathogenic fungus

Bignell

Cairns²,

Throckmorton

et al. 2016

Phil. Trans. R. Soc. B

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“…New species, useful for biotechnology applications, have been reported to produce extracellular active metabolites with pharmacological interest (Bagchi and Banerjee, 2013;Pinheiro et al, 2013). These microorganisms can also protect plants against phytopathogens, temperature, drought and world weather changes (Gundel et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated that endophytic fungi can produce numerous compounds with biological activities of interest, such as antitumor (Jin-long et al, 2011;Chandra, 2012), antimicrobial (Siqueira et al, 2011;Tayung et al, 2012;Bagchi and Banerjee, 2013;Pinheiro et al, 2013;Orlandelli et al, 2015), enzymes (Chandra, 2012), plant growth hormones (Hwang et al, 2011), leishmanicidal (Santiago et al, 2012), cytotoxic compounds and compounds with industrial and pharmaceutical potential (Meng et al, 2011;Wang and Dai, 2011), reinforcing the broad biotechnological potential of these microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Silva¹,

Silvino²,

Bezerra³

et al. 2017

Afr. J. Microbiol. Res.

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The discovery of new metabolites potentially bioactive against pathogenic microorganisms, mainly multidrug resistant, has aroused interest in endophytic fungi. The plant-associated microorganisms have been an important source for development of new compounds of biotechnological interest. This study aimed to investigate the antibacterial capacity of the endophytic fungus, Phoma sp. URM 7221 isolated from the medicinal plant Schinus terebinthifolius against human-pathogenic bacteria. An endophyte was isolated from S. terebinthifolius leaves. Phoma herbarum URM7221 was characterized morphologically and on the basis of ITS rDNA sequence. Primary antimicrobial activity was evaluated using the agar diffusion method and fermentation in liquid medium. Six different solvents were used to extract the active metabolites from fungal biomass and metabolic liquid. An antimicrobial activity test from the extract was carried out using a disk diffusion method with the endophytic extract containing the best antibacterial activity. Two tests were performed: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Partially purified secondary metabolite extracts were analysed by thin layer chromatography (TLC). Liquid metabolically bioactive compounds extracted with petroleum ether revealed a MIC of 25 and 500 μg.mL −1 against S. aureus and MRSA, respectively. Ether and methanol extracts were assessed by chemical analyses and contained phenolic compounds, triterpenes, steroids, reducing sugars, mono-and sesquiterpenes. The thin layer chromatography assay showed the activity of different antimicrobial compounds produced by Phoma sp. URM7221. This endophyte (URM7221) could be efficiently used for production of bioactive metabolites against pathogenic microorganisms, with significant biotechnological potential.

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“…Both are broad-spectrum antibacterial agents, however, fumigaclavine C was the most active against B. subtilis showing MIC of 7.81 mg/ml, while pseurotin showed MIC 15.62 mg/ ml. [49] The mangrove endophyte Aspergillus sp. 16-5c produced a sesterterpenoid, asperterpenoid A (60).…”

Section: Endophyte-host Interactionmentioning

confidence: 99%

Metabolomic Tools to Assess the Chemistry and Bioactivity of Endophytic Aspergillus Strain

Tawfike

Tate

Abbott

et al. 2017

Chemistry & Biodiversity

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Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical and agrochemical drugs. In this study, a combination of metabolomics and bioactivity-guided approaches were employed to isolate secondary metabolites with cytotoxicity against cancer cells from an endophytic Aspergillus aculeatus. The endophyte was isolated from the Egyptian medicinal plant Terminalia laxiflora and identified using molecular biological methods. Metabolomics and dereplication studies were accomplished by utilizing the MZmine software coupled with the universal Dictionary of Natural Products database. Metabolic profiling, with aid of multivariate data analysis, was performed at different stages of the growth curve to choose the optimized method suitable for up-scaling. The optimized culture method yielded a crude extract abundant with biologically-active secondary metabolites. Crude extracts were fractionated using different high-throughput chromatographic techniques. Purified compounds were identified by HR-ESI-MS, 1D- and 2D-NMR. This study introduced a new method of dereplication utilizing both high-resolution mass spectrometry and NMR spectroscopy. The metabolites were putatively identified by applying a chemotaxonomic filter. We also present a short review on the diverse chemistry of terrestrial endophytic strains of Aspergillus, which has become a part of our dereplication work and this will be of wide interest to those working in this field.

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Antibacterial activity of alkaloids produced by endophytic fungusAspergillussp. EJC08 isolated from medical plantBauhinia guianensis

Cited by 89 publications

References 13 publications

Secondary metabolite arsenal of an opportunistic pathogenic fungus

Secondary metabolite arsenal of an opportunistic pathogenic fungus

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Metabolomic Tools to Assess the Chemistry and Bioactivity of Endophytic Aspergillus Strain

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