Antibacterial activity of caffeine against plant pathogenic bacteria

Śledź, Wojciech; Los, Emilia; Paczek, Agnieszka; Rischka, Jacek; Motyka, Agata; Żołędowska, Sabina; Piosik, Jacek; Łojkowska, Ewa

doi:10.18388/abp.2015_1092

Cited by 48 publications

(34 citation statements)

References 21 publications

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“…N-OH-Pip Is Not Bactericidal. To rule out that N-OH-Pip is toxic to bacterial growth in planta, we performed a minimum inhibitory concentration (MIC) assay (33) to measure potential bactericidal activity. Cultures of Psm ES4326, Pst DC3000, and Pst avrRpt2 (13,21,22).…”

Section: N-oh-pip Treatment Of Arabidopsis Leaves Is Sufficient To Inmentioning

confidence: 99%

N -hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis

Chen

Holmes

Rajniak

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

224

294

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Systemic acquired resistance (SAR) is a global response in plants induced at the site of infection that leads to long-lasting and broad-spectrum disease resistance at distal, uninfected tissues. Despite the importance of this priming mechanism, the identity and complexity of defense signals that are required to initiate SAR signaling is not well understood. In this paper, we describe a metabolite, -hydroxy-pipecolic acid (-OH-Pip) and provide evidence that this mobile molecule plays a role in initiating SAR signal transduction in We demonstrate that FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1), a key regulator of SAR-associated defense priming, can synthesize-OH-Pip from pipecolic acid , and exogenously applied-OH-Pip moves systemically in and can rescue the SAR-deficiency of mutants. We also demonstrate that -OH-Pip treatment causes systemic changes in the expression of pathogenesis-related genes and metabolic pathways throughout the plant and enhances resistance to a bacterial pathogen. This work provides insight into the chemical nature of a signal for SAR and also suggests that the-OH-Pip pathway is a promising target for metabolic engineering to enhance disease resistance.

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Section: N-oh-pip Treatment Of Arabidopsis Leaves Is Sufficient To Inmentioning

confidence: 99%

N -hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis

Chen

Holmes

Rajniak

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

224

294

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show abstract

“…MIC assays were performed according to (Sledz, 2015) with slight modification. A single colony of Psm ES4326 or Pst DC3000 was incubated in LB broth, while a single colony of Pst avrRpt2 was incubated in NYGA broth without antibiotics and shaken (220 rpm) at 28°C for 24 hr.…”

Section: Minimum Inhibitory Concentration (Mic) Assaymentioning

confidence: 99%

N-hydroxy-pipecolic acid is a mobile signal that induces systemic disease resistance in Arabidopsis

Chen

Holmes

Rajniak

et al. 2018

Preprint

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20Systemic acquired resistance (SAR) is a global response in plants induced at the site of 21 infection that leads to long-lasting and broad-spectrum disease resistance at distal, 22 uninfected tissues. Despite the importance of this priming mechanism, the identity of the 23 mobile defense signal that moves systemically throughout plants to initiate SAR has 24 remained elusive. In this paper, we describe a new metabolite, N-hydroxy-pipecolic acid 25 (N-OH-Pip), and provide evidence that this molecule is a mobile signal that plays a 26 central role in initiating SAR signal transduction in Arabidopsis thaliana. We 27 demonstrate that FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1), a key 28 regulator of SAR-associated defense priming, can synthesize N-OH-Pip from pipecolic 29 acid in planta, and exogenously applied N-OH-PIP moves systemically in Arabidopsis 30 and can rescue the SAR-deficiency of fmo1 mutants. We also demonstrate that N-OH-31 Pip treatment causes systemic changes in the expression of pathogenesis-related 32 genes and metabolic pathways throughout the plant, and enhances resistance to a 33 bacterial pathogen. This work provides new insight into the chemical nature of a mobile 34 signal for SAR and also suggests that the N-OH-Pip pathway is a promising target for 35 metabolic engineering to enhance disease resistance. 36 37the establishment of SAR signaling [10, 12, 13]. The biochemical function of FMO1 has 68 remained unknown. 69Several forward genetic screens searching for SAR-deficient mutants identified 70 multiple alleles of fmo1, ald1 (AGD2-LIKE DEFENSE RESPONSE PROTEIN 1), and 71 sard4 (SAR-DEFICIENT 4), highlighting the importance of metabolites produced by 72 these enzymes [16, 17, 19, 20]. ALD1 and SARD4 are involved in the biosynthesis of 73 Pip [13, 21, 22]. Irrigation of wild type Arabidopsis plants with Pip induces SAR [13], 74 which suggested that Pip might be a mobile SAR metabolite. Navarova et al. reported, 75 however, that Pip could not trigger SAR in fmo1 mutants [13]. In addition, fmo1 plants 76 accumulate high levels of Pip during a late stage of infection compared to wild-type 77 plants [13]. These findings feature FMO1 as a key missing link in the mechanism of Pip-78 associated SAR. 79 We and others have found untargeted metabolite analysis of Arabidopsis genetic 80 mutants to be a powerful approach for the identification of small molecules associated 81 with fitness phenotypes (either previously characterized or suggested by transcriptome 82 analysis) [23]. Examples include the identification and characterization of cytochromes 83 P450 involved in phytoalexin production [24] and iron acquisition [25]. Given that FMO1 84 is one of the genes most responsive to biotic stress (as indicated by analysis of 85 previously reported Arabidopsis microarray data summarized in Fig. S9), and that 86 genetic data suggest molecules generated by FMO1 are required for initiating SAR [16-87 18], we sought to apply an untargeted metabolomics approach to determine the 88 products of FMO1 and their ...

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“…glycinea was correlated with the increasing concentration of this compound [11]. Subsequently, Sledz et al [12] evaluated antibacterial activity of caffeine toward broad spectrum of plant pathogenic bacteria causing economic losses in crop and ornamental plant production worldwide: Clavibacter michiganensis subsp. sepedonicus (Cms), Dickeya solani (Dsol), Pectobacterium atrosepticum (Pba), Pectobacterium carotovorum subsp.…”

Section: Introductionmentioning

confidence: 99%

“…Caffeine inhibited growth of the above-listed phytopathogens in broth cultures, increased their generation time, triggered morphological abbreviations, and finally exhibited bactericidal effect in concentrations from 40 to 100 mM [12]. Moreover, application of this compound reduced disease symptoms caused by Dsol on potato slices, whole potato tubers, and chicory leaves [12]. In addition, it was shown that the plant pathogenic bacterium tested could not develop any resistance to the caffeine treatment [12].…”

Section: Introductionmentioning

confidence: 99%

Influence of Exogenously Supplemented Caffeine on Cell Division, Germination, and Growth of Economically Important Plants

Śledź¹,

Motyka²,

Żołędowska³

et al. 2017

The Question of Caffeine

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Caffeine is a plant secondary metabolite of antiherbivory, allelopathic, and antibacterial activity. In our previous study, caffeine was shown to be an effective agent toward plant pathogenic bacteria causing high economic losses in crop production worldwide. Current study indicated that growth media supplementation with soil or plant extract did not interfere with antibacterial action of caffeine against Clavibacter michiganensis, Dickeya solani, Pectobacterium atrosepticum, Pectobacterium carotovorum, Pseudomonas syringae, Ralstonia solanacearum, and Xanthomonas campestris. The impact of caffeine on plant cell division, seed germination and growth of economically important plants was evaluated to assess possible applicability of caffeine in plant protection field. Caffeine impaired plant cell division process and inhibited in vitro germination of tomato and lettuce. Regeneration of potato explants was also negatively affected by the addition of caffeine. However, caffeine spraying or watering of tomato, lettuce and cabbage grown in soil did not impair plant development. Although the tested plants accumulated caffeine, its inner quantity was reduced by peeling and/or cooking. According to the results, caffeine warrants additional attention as a useful, natural compound designated for the control of bacterial plant pathogens. Proposed treatment seems promising especially in the case of providing protection for overwinter-stored table potato tubers.

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Antibacterial activity of caffeine against plant pathogenic bacteria

Cited by 48 publications

References 21 publications

N -hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis

N -hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis

N-hydroxy-pipecolic acid is a mobile signal that induces systemic disease resistance in Arabidopsis

Influence of Exogenously Supplemented Caffeine on Cell Division, Germination, and Growth of Economically Important Plants

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