Antibacterial activity of cationic proteins from human granulocytes.

Odeberg, Håkan; Olsson, Inge

doi:10.1172/jci108186

Cited by 204 publications

(119 citation statements)

References 31 publications

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“…aureus the antibacterial effect of cathepsin G peaked at 30 min and diminished with continued incubation time until at 3 h the cathepsin G appeared to be bacteriostatic. This is similar to the killing seen by Odeberg & Olsson (1975), when their dilutions are taken into consideration. In contrast, cathepsin G continued to exhibit extensive listericidal activity during the entire 3 h incubation period, with 79% killing at 3 h. The apparent escape of S. aureus from the microbicidal effect of cathepsin G was not due to a faster growth of this organism, as the growth rate of untreated S. aureus was similar to that of L. monocytogenes for the entire 3 h incubation period.…”

Section: Resultssupporting

confidence: 78%

Listericidal activity of human neutrophil cathepsin G

Alford

Amaral

Campbell

1990

Journal of General Microbiology

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Section: Resultssupporting

confidence: 78%

Listericidal activity of human neutrophil cathepsin G

Alford

Amaral

Campbell

1990

Journal of General Microbiology

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“…They eliminate pathogens by phagocytosis, the release of reactive oxygen intermediates and activation of proteolytic enzymes [26,28]. Similar cellular processes have been described for phagocytes of insects, with receptors on the surface of plasmatocytes and granulocytes exhibiting striking similarity to receptors on mammalian phagocytes [14].…”

Section: Discussionmentioning

confidence: 82%

Correlation between Gliotoxin Production and Virulence of Aspergillus fumigatus in Galleria mellonella

et al. 2004

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Aspergillus fumigatus is a pathogenic fungus capable of causing both allergic lung disease and invasive aspergillosis, a serious, life-threatening condition in neutropenic patients. Aspergilli express an array of mycotoxins and enzymes which may facilitate fungal colonisation of host tissue. In this study we investigated the possibility of using the insect, Galleria mellonella, for in vivo pathogenicity testing of Aspergillus species. Four clinical isolates of Aspergillus fumigatus and a single strain of Aspergillus niger were characterised for catalase and elastase activity and for the production of gliotoxin. Gliotoxin is an immunosuppressive agent previously implicated in assisting tissue penetration. Results illustrated a strain dependent difference in elastase activity but no significant difference in catalase activity. Gliotoxin production was detected in vitro and in vivo by Reversed Phase-High Performance Liquid Chromatography, with highest amounts being produced by A. fumigatus ATCC 26933 (350 ng/mg hyphae). Survival probability plots (Kaplan-Meier) of experimental groups infected with Aspergillus conidia indicate that G. mellonella is more susceptible to fungal infection by A. fumigatus ATCC 26933, implicating a critical role for gliotoxin production rather than growth rate or enzymatic activity in the virulence of A. fumigatus in this model.

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“…Nevertheless, these experiments cannot definitely rule out a direct role for cationic proteins in damage to pseudohyphae. In addition, cationic proteins, by their activity on the cell surfaces and metabolism of microorganisms (55), may have synergistic effects with other microbicidal mechanisms (56). Because cationic proteins are released from neutrophils, they may also result in activation of the complement system and generation of chemotactic factors, and thereby play an important role in neutrophil anti-Candida function (57).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Daimond¹,

Krzesicki²

1978

J. Clin. Invest.

121

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A B S T R A C T Mechanisms were studied that might explain the attachment and damage to Candida albicans pseudohyphae by neutrophils in the absence of serum. Attachment of neutrophils to pseudo hyphae was inhibited by Candida mannans (1-10 mg/ml), but not by mannose, dextran, chitin, conconavalin A, or highly charged polyamino acids. Contact was also inhibited by pretreatment of Candida before incubation with neutrophils with chymotrypsin, but not trypsin or several inhibitors of proteases. Similar results were obtained with pretreatment of neutrophils, except that trypsin was inhibitory. When pseudohyphae were killed with ultraviolet light, proteinpolysaccharide complexes of mol wt <10,000 were released which appeared to bind to the surfaces of neutrophils and inhibit contact between neutrophils and Candida, as well as other fungi.Damage to Candida by neutrophils was inhibited by agents known to act on neutrophil oxidative microbicidal mechanisms, including sodium cyanide, sodium azide, catalase, superoxide dismutase, and 1, 4 diazobicyclo (2, 2, 2) octane, a singlet oxygen quencher. Neutrophils from a patient with chronic granulomatous disease did not damage Candida at all. However, the hydroxyl radical scavengers mannitol and benzoate were not inhibitory. Cationic proteins and lactoferrin also did not appear to play a major role in this system. Low concentrations of lysozyme which Preliminary findings were presented in part at the Annual

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Antibacterial activity of cationic proteins from human granulocytes.

Cited by 204 publications

References 31 publications

Listericidal activity of human neutrophil cathepsin G

Listericidal activity of human neutrophil cathepsin G

Correlation between Gliotoxin Production and Virulence of Aspergillus fumigatus in Galleria mellonella

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

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