Antibacterial activity of chlorophyll polymeric form against test cultures S. aureus and E. coli

Abstract

The increase in the number of microorganism strains with resistance to anti-bacterial and disinfecting agents is getting more and more prevalent and has become an issue when treating the human and animals diseases and carrying out measures for disinfection treatment. This problem can be solved by using photodynamic and light-independent therapy. In both areas, metalloporphyrins have been successfully used for many years. One of the most famous representatives of porphyrins is chlorophyll (Chl). This work aims to develop of Chl polymeric form by incorporation in poly(lactic acid) (PLA) and study its inhibitory effect against Staphylococcus aureus and Escherichia coli, which are known as contaminants of the mucous and skin epithelium of humans and animals. The preparation method of Chl polymeric form is presented. The degree of Chl incorporation into PLA was more than 98%. The assessment of antimicrobial activity was carried out by measuring the inhibition zone diameters after bacterial incubation for 24–96 h. It was shown that Chl and Chl-PLA at a dosage of 75 µg inhibited S. aureus significantly. The exclusively bacteriostatic effect on E. coli was observed. These results can be used in the development of dosage forms and disinfectants.