Antibacterial Activity of Copaiba Oil Gel on Dental Biofilm

Abstract

Amazonian biodiversity products that have been used for years in folk medicine, have emerged as feasible and promising alternatives for the inhibition of microorganisms in dental biofilm. Copaiba oil, a phytotherapic agent widely used by the Amazonian populations, is known for its antibacterial, anti-inflammatory, anesthetic, healing and antitumor medicinal properties.Objective:The aim of this study was to evaluate the in vitro antibacterial activity of copaiba oil (Copaifera multijuga) gel against strains of Streptococcus sp present in dental biofilm.Materials and Methods:The copaiba oil was obtained and the chemical components were identified. The oil emulsions were formulated and used with the Brain Heart Infusion agar diffusion method with strains of Streptococcus mitis, Streptococcus constellatus and Streptococcus salivarius isolated from patients as well as standard strains of S. mitis (ATCC903), S. mutans (ATCC10449), S. sanguinis (ATCC15300) and S. oralis (ATCC10557). The study groups were as follows: experimental copaiba oil gel, 1% chlorhexidine gel (positive control) and base gel (negative control). The seeded plates were incubated at 37ºC for 12, 24 and 48 hours, respectively. The results obtained were analyzed by Shapiro-Wilk and Friedman Tests (p<0.05) for non parametric data and the Tukey test was used for pH values with 5% level of significance. Results:The experimental copaiba oil gel and 1% chlorhexidine gel showed antibacterial activity against the tested microorganisms.Conclusion: The copaiba oil gel demonstrated antibacterial activity against all the strains of Streptococcus sp tested, suggesting that it can be used for dental biofilm control.

Antimicrobial activity of copaiba oil (Copaifera ssp.) on oral pathogens: Systematic review

“…It was observed in this analysis that all studies presented an unclear risk of bias for randomization, allocation concealment, and blinding of the evaluators. Hence, appropriate statistical analysis was performed only in five of the 11 featured items (Bardají et al , ; Bari et al , ; Dias et al , ; Pieri et al , ; Simões et al , ). In addition, in two other articles, the statistical analysis is not clear (Souza et al, ; Souza et al, ).…”

“…On the other hand, these chemical products showed bactericidal and bacteriostatic actions, which make it an important metabolite in the search for an effective antimicrobial agent. Most of the copaiba species showed a lower antibacterial activity when compared to chlorhexidine (Bardají et al, ; Conde et al, ; da Silva Moraes et al, ; Dias et al, ; Pieri et al, ; Simões et al, ).…”

“…Four thousand five hundred and thirty-five titles/abstracts were retrieved from the search strategy, of which 2,474 were excluded for being duplicated. Based on the previously described criteria, 84 full titles were evaluated, of which 11 were selected (Bardají et al, 2016;Bari et al, 2016;Conde et al, 2015;da Silva Moraes et al, 2016;Dias et al, 2015;Pieri et al, 2010;Pieri, Mussi, Fiorini, Moreira, & Schneedorf, 2012;Simões et al, 2016;Vasconcelos, Veiga Júnior, Rocha, & Bandeira, 2008).…”

Effects of a nanoemulsion with Copaifera officinalis oleoresin against monogenean parasites of Colossoma macropomum: A Neotropical Serrasalmidae

“…b-caryophyllene is the main constituent of Copaifera spp. oleoresin (Cascon & Gilbert, 2000;Simões et al, 2016). This sesquiterpene is a chemical marker for Copaifera species, and it is responsible for several forms of biological activity of the oleoresin of these plants (Lima et al, 2011;Tappin et al, 2004).…”

“…Copaifera spp. are Amazon plants known for their antibacterial, anthelmintic and antiprotozoal properties (Costa et al, 2017;Rodrigues et al, 2014;Simões et al, 2016). It is a mixture of several substances, among which the sesquiterpene b-caryophyllene is the main constituent (Lima et al, 2011;Veiga-Junior, Zunino, Patitucci, Pinto, & Calixto, 2006).…”