Antibacterial activity of crude extracts Ulva lactuca against Staphyloccocus aureus and Propionibacterium acne

Abstract

Sources of antibiotic raw materials as a reliever / acne remover drug is needed. Especially of natural ingredients from the Ulva lactuca. This study aims to examine the potential of crude extracts of U. lactuca as an antibacterial against Staphyloccocus aureus and Propionibacterium acne. Extraction of U. lactuca used Microwave Assited Extraction (MAE) technique with different types of solvents and extraction time. Analysis of antibacterial activity was carried out by the paper disk diffusion method. The research design used was a Factorial Completely Randomized Design (CRD) with two factors. The first factor of type solvent;ethanol, ethyl acetate and aquades. The second factor of extraction time which consists of 3 different levels;10 minutes, 20 minutes, and 30 minutes. The results of the study The highest yield was obtained in ethanol by 30 minutes extraction time;average of 24.9%. Phytochemical compounds were detected used a color test on the extract of Ulva lactuca namely alkaloids, steroids, flavonoids, and tannins. Whereas the antibacterial test with the highest tasteless zone of Staphylococcus aureus bacteria with ethanol solvent extraction time of 30 minutes;average of 17.17 mm. Crude extract of U. lactuca has potential as an antibacterial S. aureus and P. acne.