This study was conducted in Hillah city, Babil province in Iraq, to know the extent of the effects of the secondary metabolites, such as flavonoids and terpenoids extract from Eruca sativa Mill leaves, against some bacterial species isolated Prostatitis Samples represented by Escherichia coli, Staphylococcus aureus, streptococcus pyogenes, Pseudomonas aeruginosa, and Klebsiella pneumonieae. An ability of antibacterial was totally completed by utilizing the method of agar well diffusion by preparing three concentrations 25, 50 &100mg/ml. sterile distal water was utilized as a negative control. Flavonoid extract at 50 and 100 mg/ml exhibited significant supremacy at Probability ≤ 0.05 over the negative control when applied to Escherichia coli. Staphylococcus aureus was sensitive to flavonoids and terpenoid compounds at all concentrations under study P≤ 0.05 in comparison with the negative control. Whereas streptococcus pyogenes, Pseudomonas aeruginosa, and Klebsiella pneumonieae are totally resistant to all concentrations of flavonoid compounds and Escherichia coli, Klebsiella pneumonieae, and Pseudomonas aeruginosa fully resistance to all concentration of terpenoid compounds. Lastly, flavonoids and terpenoid compounds in leaves of Eruca sativa respected a good source for controlling some bacterial species isolated from Prostatitis samples, especially against Staphylococcus aureus.