Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566

Abstract

Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.

Antibacterial Effects of Sarang Semut (Myrmecodia Pendans) Fractions Using Three Different Solvents Toward Enterococcus Faecalis Cps2

“…Fatriadi et al stated that the ethyl acetate fraction of sarang semut had antibacterial activity against Streptococcus sanguinis. Kurnia et al investigated the ethyl acetate fraction of sarang semut toward Porphyromonas gingivalis[33,34].…”

Antibacterial alternatives using the potential of the ant nest plant (Myrmecodia spp.)

“…The ethyl acetate extract of the ant nest plant was identified as containing phenolics, tannins, flavonoids, and terpenoids through phytochemical analyses. The antibacterial activity of the ethyl acetate fraction against S. sanguinis was stronger than chlorhexidine gluconate, with MIC values of 31.25 ppm and 0.49 ppm, respectively [42]. The aqueous extract of Myrmecodia spp.…”

AKTIVITAS ANTIAKTERI EKSTRAK ETANOL BIJI DAN BUAH KUPA (Syzygium polichepalum (miq.) Merr.& perry) TERADAP BAKTERI PENYEBAB JERAWAT

“…Aktivitas antibakteri ekstrak etanol biji dan daging buah kupa diprediksi berasal dari senyawa metabolit sekunder yang terkandung di dalamnya, yaitu berdasarkan pengujian skrining fitokimia, diketahui bahwa ekstrak etanol biji dan daging buah kupa mengandung alkaloid, flavonoid, kuinon, tanin, polifenol, dan golongan terpen. Berdasarkan pustaka, disebutkan bahwa senyawa metabolit sekunder seperti alkaloid, flavonoid, kuinon, tanin, polifenol dan golongan terpen tersebut mempunyai mekanisme aktivitas antibakteri (Alibi et al, 2021;Bufo & Karaman, 2019;Fatriadi et al, 2018;Mahizan et al, 2019) Adapun mekanisme senyawa flavonoid, tanin dan polifenol dapat mendenaturasi protein dinding sel bakteri, sifat polaritas gugus hidroksinya dapat menghambat pembentukan asam amino sehingga merusak membran sel dan metabolisme sel terganggu yang pada akhirnya sel bakteri akan mengalami lisis (Fatriadi et al, 2018). Sedangkan senyawa kuinon mampu membentuk kompleks yang ireversibel dengan asam amino sehingga menyebabkan inaktivasi dan kehilangan fungsional protein pada permukaan adhesif sel, polipeptida dinding sel, serta membran sel yang berikatan dengan enzim (Alibi et al, 2021) (Klančnik et al, 2010).…”