Antibacterial Activity of Geranium Pratense Preparations

Abstract

Geranium pratense is a perennial herb with horizontal rootstock, palmately lobed leaves and pentamerous zygomorphic bluish flowers, widespread in Russia. The chemical composition of the aerial part and root part of G. pratense includes hydrolysable and condensed tannins, phenolic acids, flavonoids. herb, rhizome and root of G. pratense in powder or by infusion or decoction are reportedly used in folk medicine in treatment of periodontal diseases and stomatitis. The aim of the present investigation was to determine optimal conditions for extraction of biologically active compounds from G. pratense, to evaluate antimicrobial activity of powdered extracts obtained from its aerial part and root part and to validate scientifically its use in traditional medicine. The plant matter (herb or rhizome and root of G. pratense, collected in 2010-2011 in Northwest Russia) was extracted with 40 % ethanol. Trials varying in methods of extraction, particle size, extraction time and solvent to solid ratio were performed. The quantitative evaluation of tannins and flavonoids in 40 % ethanol aqueous extracts and powdered extracts was carried out by spectrophotometric method. Results of assays were reported in gallic acid equivalents for tannins and rutin equivalents for flavonoids. broth dilution method followed by subculturing to agar media without antibacterial agent was employed to assess minimal inhibition concentration (MiC) and minimal bactericidal concentration (MbC). Optimal conditions for extraction of phenolic constituents from raw material were evaluated. Powdered herb extract (PhE) was reported to contain 33.05 % of tannins and 9.74 % of flavonoinds. Antimicrobial studies suggested that PhE had inhibitory effect against S. aureus and B. cereus: the MiC was found to be 2 mg/ml against S. aureus, the Mib against B. cereus was 1 mg/ml. The total content of tannins in powdered extract of G. pratense root part (PRE) is 41.56 %. PRE exhibited growth inhibitory activity against S. aureus, B. cereus and P. aeruginosa with Mib value of 0.5 mg/ml, 1 mg/ml and MiC value of 2 mg/ml respectively. PhE and PRE revealed antimicrobial activity against Gram-positive bacteria. The existence of microorganisms in particular Streptococcus sp. in an oral biofilm and dental plaque has profound implications for the etiology of periodontal disease. So the results indicate that the studied G. pratense preparations could become a new effective agent in a wide range of oral care products used in treatment-and-profilactic of periodontitis, gingivitis and stomatitis. DEVELOPMENT OINTMENT AND SUPPOSITORY WITH HYPERICUM PERFORATUM HERB POLYEXTRACT St. Petersburg State Chemical-Pharmaceutical Academy, Russiahypericum perforatum herb (John's wort) contains a variety of biologically active substances (bAS), such as hypericin, pseudohypericin, hyperforin, flavonoids, carotenoids, vitamins, etc. Great "ensemble" of St. John's wort's bAS causes a wide range of its therapeutic action. There are not technologies a soft pharmaceutical form with hydroalcoholic...