Antibacterial Activity of Glutathione-Coated Silver Nanoparticles against Gram Positive and Gram Negative Bacteria

Taglietti, Angelo; Fernandez, Yuri Diaz; Amato, Elvio D.; Cucca, Lucia; Dacarro, Giacomo; Grisoli, Pietro; Necchi, Vittorio; Pallavicini, Piersandro; Pasotti, Luca; Patrini, M.

doi:10.1021/la3003838

Cited by 292 publications

(241 citation statements)

References 59 publications

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“…In these cases, the peptidoglycan thickness plays an important role in the interaction with the nanoparticles, but this effect might be modulated by the other features of the environment, such as pH, the metal structure or the carrier Allaker (2010), Seil and Websters (2012), Wang et al (2012). A similar effect has been observed in the study of Spacciapoli et al (2001), Sondi and Salopek-Sondi (2004), Taglietti et al (2012). A ten times higher concentration was obtained for Ag for Bacillus cells in neutral pH=7.0 than that of Pseudomonas at slightly acidic pH=6.0 and a longer time for the bacterial contact killing -4 h for Bacillus in contrast with Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 56%

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Stoyanova¹,

Ivanova²,

Angelov³

et al. 2017

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This article aims to explore the antibacterial activity of thin films of TiO doped with Ag and Cu using two types of Gram-negative and Gram-positive test bacteria with clinical significance (Gracilicutes and Firmcutes bacteria). The thin films (thickness of about 60 nm) were deposited on glass substrates by radio frequency magnetron co-sputtering (r.f. power of 50 W) of TiO target with Ag and Cu pieces on its surface in an Ar atmosphere (0.8 Pa) without heating during the deposition. The total surface area of the Ag was 60 mm and that of the Cu was 100 mm . Bacillus cereus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli and Pseudomonas sp. were used as test strains. The antibacterial actvity of the films was evaluated by the classical Koch's method and optical density measurements. The bactericidal effect was established at different time points between 30 min and 90 min for Pseudomonas sp. and S. enterica. The Firmicutes bacteria B. cereus and S. epidermidis were killed at the 4 and 8 hour of the treatment, respectively. The effect on E. coli was bacteriostatic until the 10 hour. The results were confirmed by assessment of the bacterial dehydrogenase activity. The studied thin films of TiO co-doped with Ag and Cu have a potential for application as antibacterial coatings.

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Section: Discussionmentioning

confidence: 56%

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Stoyanova¹,

Ivanova²,

Angelov³

et al. 2017

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“…This was supported by Taglietti et al [21] who described a 'short distance' interaction mechanism of local physical action of the Ag nanoparticles and 'a long distance' ion release mechanism.…”

Section: Introductionmentioning

confidence: 72%

“…Depending on the properties of this capping agent, the characteristics (geometry and solubility) of the nanoparticles can be controlled. The choice of capping agent must be directed by the characteristics and application of the intended nanoparticles; for biological applications, polypeptides (gluthatione) [21,45,46], tiopronin [10,33] and aminoacids have been previously employed [31,45]. The common feature of these compounds is the presence of sulphur whose strong affinity for Ag results in coverage of the nanoparticles and provides stability during nanoparticles ripening.…”

Section: Discussionmentioning

confidence: 99%

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Perni

Hakala

Prokopovich

2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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The number of medical applications of silver nanoparticles is constantly expanding due to their high bactericidal properties coupled with low toxicity towards mammalian cells. Because of this expanding use of silver nanoparticles, novel methods of synthesis have been developed in order to achieve nanoparticles preparation through inexpensive and environmentally friendly processes; biogenic synthesis of silver nanoparticles is an approach that meets those requirements.In this work, E. coli cultures centrigugates were used to synthesis L-cysteine capped silver nanoparticles. The ratio between AgNO 3 and L-cysteine has been proven to affect the size of the nanoparticles: higher amount of L-cysteine returns smaller nanoparticles, but does not affect the percentage of organic matter in the nanoparticles as determined through TGA.The silver nanoparticles prepared had antimicrobial activity against E. coli and S. aureus with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) independent of the nanoparticles size; for both bacterial species the values of MIC and MBC were the same, 0.0432 mg/ml for E. coli and 0.0216 mg/ml for S. aureus.

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“…However, a lower inactivation rate of 99.83% was achieved against S. aureus. As reported previously, S. aureus owns a thicker peptidoglycan layer of the cell wall preventing the penetration of Ag NPs inside its cytoplasm [43,44], corresponding to less intense antibacterial activity. Besides, it can be interpreted by considering the nanomechanical contact effect discussed in the above section.…”

Section: Antimicrobial Activity Of Qoma Coatingsmentioning

confidence: 56%

Green antimicrobial coating based on quaternised chitosan/organic montmorillonite/Ag NPs nanocomposites

Chen

Cai³

et al. 2016

Journal of Experimental Nanoscience

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This work is aimed at developing a green antimicrobial coating. First, a green antimicrobial agent, quaternised chitosan (QCS)/organic montmorillonite (OMMT)/silver nanoparticles (Ag NPs) (QOMA) nanocomposite was fabricated through an environmental-friendly one-step approach. Morphological and structural characteristics of QOMA were investigated, and good antimicrobial activity was proved. QOMA was then incorporated into powder coating formulations to form a homogeneous coating on steel plates, which was studied by scanning electron images. Besides, the physical and mechanical properties as well as the antimicrobial performances of the coatings were discussed. The results showed that the addition of QOMA imparted good antimicrobial capacity to the powder coating, but did not affect its physical and mechanical properties. The coatings were able to effectively inactivate Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus) and fungi (Aspergillus niger, Penicillium funiculosum, Chaetomium globasum, Paecilomyces varioti, Asp. terreus and Aureobasidium pullulans). Our findings demonstrate the possibilities of green antimicrobial coating containing QOMA for practical applications in medical devices, domestic appliances and other solid surfaces concerning bacterial infection and contamination.

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Antibacterial Activity of Glutathione-Coated Silver Nanoparticles against Gram Positive and Gram Negative Bacteria

Cited by 292 publications

References 59 publications

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Green antimicrobial coating based on quaternised chitosan/organic montmorillonite/Ag NPs nanocomposites

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