Antibacterial activity of glycine betaine analogues: involvement of osmoporters

Cosquer, Anne; Ficamos, Morgane; Jebbar, Mohamed; Corbel, Jean-Charles; Choquet, Gwénaëlle; Fontenelle, Catherine; Uriac, Philippe; Bernard, Théophile

doi:10.1016/j.bmcl.2004.02.045

Cited by 28 publications

(18 citation statements)

References 13 publications

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“…1A) were highly sensitive to this compound. These observations are similar to those obtained with E. coli strains impaired in ProP and/or ProU uptake activity (12). They suggest strongly that the Tn5-B21 insertion had occurred in gene(s) encoding an osmoprotectant transport system other than the OusA transporter.…”

Section: Resultssupporting

confidence: 86%

“…BGB, a structural analogue of GB, has been shown to be toxic to E. coli strains in which either ProP or ProU was functional (12). We used this characteristic to isolate a mutant, which was resistant to BGB, from a pool of Tn5-B21 ousA::uidA-Km r E. chrysanthemi mutants.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we have exploited the characteristic of the widespread ability of microorganisms to accumulate GB to deliver structurally related compounds with antibacterial activity (12). This allowed the characterization in E. chrysanthemi of an ousB operon encoding a multicomponent, binding-proteindependent transport system involved in the high-affinity uptake of GB and choline.…”

Section: Discussionmentioning

confidence: 99%

“…The osmoprotectant solutions were sterilized by passage through a 0.45-m-pore-size sterile filter (Millipore), and these solutes were generally supplied at a concentration of 1 mM. The benzyl derivative of GB (BGB), which is chemically named carboxymethyl-dimethyl-(4-nitrobenzyl) ammonium chloride or N-benzyl-N,N-dimethylglycine betaine, was synthesized as described previously (12 ) were synthesized and purified as previously described (53). [ 14 C]ectoine (5.5 MBq mmol Ϫ1 ) was prepared biologically as described previously (34).…”

Section: Methodsmentioning

confidence: 99%

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OusB, a Broad-Specificity ABC-Type Transporter fromErwinia chrysanthemi, Mediates Uptake of Glycine Betaine and Choline with a High Affinity

Choquet

Jehan

Pissavin

et al. 2005

Appl Environ Microbiol

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The ability of Erwinia chrysanthemi to cope with environments of elevated osmolality is due in part to the transport and accumulation of osmoprotectants. In this study we have identified a high-affinity glycine betaine and choline transport system in E. chrysanthemi. By using a pool of Tn5-B21 ousA mutants, we isolated a mutant that could grow in the presence of a toxic analogue of glycine betaine (benzyl-glycine betaine) at high osmolalities. This mutant was impaired in its ability to transport all effective osmoprotectants in E. chrysanthemi. The DNA sequence of the regions flanking the transposon insertion site revealed three chromosomal genes (ousVWX) that encode components of an ABC-type transporter (OusB): OusV (ATPase), OusW (permease), and OusX (periplasmic binding protein). The OusB components showed a significant degree of sequence identity to components of ProU from Salmonella enterica serovar Typhimurium and Escherichia coli. OusB was found to restore the uptake of glycine betaine and choline through functional complementation of an E. coli mutant defective in both ProU and ProP osmoprotectant uptake systems. Competition experiments demonstrated that choline, dimethylsulfoniacetate, dimethylsulfoniopropionate, and ectoine were effective competitors for OusB-mediated betaine transport but that carnitine, pipecolate, and proline were not effective. In addition, the analysis of single and double mutants showed that OusA and OusB were the only osmoprotectant transporters operating in E. chrysanthemi.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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OusB, a Broad-Specificity ABC-Type Transporter fromErwinia chrysanthemi, Mediates Uptake of Glycine Betaine and Choline with a High Affinity

Choquet

Jehan

Pissavin

et al. 2005

Appl Environ Microbiol

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“…Zhang et al (1996) indicated that, in rats, betaine is involved in regulating cytokines (e.g., tumor necrosis factor-α, TNF-α) production by liver macrophages (Kupffer cells) by inhibiting the prostaglandin synthesis, suggesting a new role of organic osmolytes (especially betaine) in modulating immune function. Furthermore, Cosquer et al (2004) found that glycine betaine possesses antibacterial activities against 15 Gram-positive and Gram-negative bacteria. Presence of these properties in betaine may explain the enhancement of immune system activity.…”

Section: Humoral and Cellular Immunitymentioning

confidence: 98%