Antibacterial activity of human amniotic fluid

Bergman, Nira; Bercovici, B; Sacks, T.

doi:10.1016/0002-9378(72)90214-1

Cited by 51 publications

(22 citation statements)

References 6 publications

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“…In our study, microorganisms grew in 86.7% of fluid specimens. This frequency is high and may be explained by inopportune vaginal bacteria collected during catheterism, a de crease in activity of the amniotic fluid when the inoculum size was increased [1,11], or by the absence of the bactericidal polypeptide linked to zinc [16]. No anaerobic bacteria were isolated.…”

Section: Discussionmentioning

confidence: 97%

Quantitative Bacteriological Analysis of Amniotic Fluid

et al. 1982

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Quantitative bacteriological analysis of amniotic fluid (AF) was performed on 60 fluid samples collected by catheter from 50 selected labor patients regarded as liable to infection. AF cultures were positive in 52 cases. The bacterial colony counts ranged from less than 102 colony-forming units (CFU) per milliliter to 10⁹ CFU/ml. Pathogenic bacteria in neonates such as group B Streptococcus, Escherichia coli, and Listeria monocytogenes were encountered with numerations equal to or greater than 10⁷ CFU/ml in groups with clinical findings. An excellent correlation was noted between bacterial counts in AF and clinical data (p < 0.00006). The results confirm that the quantitative bacteria analysis of the AF is a useful tool for evaluation of an infection risk for neonates.

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Section: Discussionmentioning

confidence: 97%

Quantitative Bacteriological Analysis of Amniotic Fluid

et al. 1982

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“…The bactericidal activity was measured by the method described by Bergmann et al [16] . The peritoneal fluid collected by using PBS was centrifuged at 900 r/min for 15 min at 4℃.…”

Section: Methodsmentioning

confidence: 99%

Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats

Çerçi¹

2007

WJG

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activity of peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. G-CSF increased both bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid, and prevented the bacterial translocation. We conclude that intraperitoneal GCSF administration protects the effects of systemic 5-FU on peritoneal defense mechanisms. INTRODUCTIONChemotherapy is one of the most important methods in the therapy of malignant diseases. It has been widely used and it is well known that systemic chemotherapy has immunosuppressive effects [1] . 5-Fluorouracil (5-FU) has side effects, including leucopoenia, stomatitis, appetite loss, diarrhea, and mild fever like other chemotherapy agents [2,3] . It has been reported that a high dose of 5-FU often induces cytotoxicity in intestinal tissue that results in ulceration, diarrhea, and bacterial translocation [4][5][6][7][8] .Bacterial translocation is one of the most important causes of sepsis and multiple organ failure. Intestinal mucosa has a barrier function that prevents the colonization of the bacteria and the passage of bacteria and their toxins from the intestinal system into the systemic circulation. It has been shown that bacterial translocation is also augmented by shock, mesenteric ischemia, thermal injury, malnutrition, obstructive jaundice, and intestinal obstruction [9][10][11][12][13][14] . Granulocyte-colony stimulating factor (G-CSF) is a cytokine that is used to reverse the neutropenia associated with cytotoxic chemotherapy bone marrow and haemopoietic stem cell transplantation [15] . Abstract AIM: To investigate the effects of granulocyte-colony stimulating factor (G-CSF) on peritoneal defense mechanisms and bacterial translocation after systemic 5-Fluorouracil (5-FU) administration.

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“…The presence and antimicrobial activity of lysozyme in many tissues, body fluids and secretions, including amniotic fluid, vaginal fluid and cervical mucus plug [3,7,8,10,11,25,32] have already been described. We used the lysozyme inhibitor imidazole [33] for indirect detection of the presence/ action of lysozyme in the HFF samples.…”

Section: Discussionmentioning

confidence: 99%

“…This study showed that HFF samples obtained from 20 patients undergoing IVF inhibited growth of Gram-positive bacteria, but not Gram-negative bacteria and yeasts [18]. Only one bacterial strain per species was included in the study and tested HFF specimens displayed almost uniform antibacterial activity [18], which has not been shown for other body fluids or secretions tested previously [3][4][5][6]9].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Human Follicular Fluids

Stepanović

Djukić

Veljković³

et al. 2003

Gynecol Obstet Invest

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The aim of this study was to explore the antimicrobial activity of human follicular fluid (HFF), to test the hypothesis that different strains of the same bacterial species could display different patterns of susceptibility to antimicrobial action of HFF, and to preliminarily investigate the possible mechanism of antimicrobial action of this fluid. Antimicrobial activity of 60 samples of HFF toward 30 Streptococcus agalactiae strains was determined by the agar diffusion method and broth dilution method. To explore the mechanism of antimicrobial activity, biochemical analyses were performed with selected fluid samples. The obtained results indicate that 38.3% fluid samples did not inhibit bacterial growth, 53.3% showed moderate and 8.3% high antimicrobial activity. The tested effect of HFF on S. agalactiae strains was bactericidal and was not strain dependent. Lysozyme activity was detected in HFF exhibiting antimicrobial activity. There were no statistically significant differences in concentrations of estradiol, progesterone, transferrin, iron, total protein and albumin levels among tested samples regardless of the different rate of antimicrobial activity. The obtained results indicate that lysozyme is most probably a crucial antibacterial agent in this fluid; however, some other still unidentified factors may contribute to it.

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Antibacterial activity of human amniotic fluid

Cited by 51 publications

References 6 publications

Quantitative Bacteriological Analysis of Amniotic Fluid

Quantitative Bacteriological Analysis of Amniotic Fluid

Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats

Antimicrobial Activity of Human Follicular Fluids

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