Antibacterial Activity of Intracellular Greenly Fabricated Selenium Nanoparticle of Lactobacillus pentosus ADET MW861694 against Selected Food Pathogens

Abstract

This study aimed at intracellular biosynthesis of selenium nanoparticles using Lactobacillus pentosusADEB (LP) and the greenly fabricated nanoparticles were characterized based on visual observation, spectroscopy, microscopy, energy dispersive X-rays, X-ray Diffraction, and dynamic light scattering properties. The antibacterial activities of the Lactobacillus pentosusADEB selenium nanoparticles (LP-SeNPs) against selected foodborne pathogens were evaluated. A gradual change in colour from brown to reddish colour indicates the formation of Nano-selenium. The highest absorption peak for LP-SeNPs was visible between 200-300nm. FTIR analysis shows major peaks around 1635.40, 1587.31 2163.00, 2936.70, 2968.50, and 3254.38 cm -1 indicate the presence of amides on the nanoparticle surface. SEM and TEM analysis shows extrusion of spherical shape SeNPS from Lactobacillus pentosus cell membrane which indicates intracellular synthesis of the nanoparticles. The nanoparticles were crystalline with an average diameter of 106.1 nm and a polydispersity index of 0.295. Elemental selenium with an absorption peak at 1.37 Kev was present in the nanoparticles. The LP-SeNPs and culture of Lactobacillus pentosus had antagonistic activities against the foodborne pathogens. The LP-SeNPs have antagonistic activity against all the test pathogens. The highest activity was against Salmonella arizonae (11.3 mm). In conclusion, the intracellular greenly fabricated LP-SeNPs had broad-spectrum antagonistic activity against the test food-borne pathogens. The addition of selenium nanoparticles to food can be an added advantage in controlling food-borne pathogens.Contribution/Originality: This study contributes to existing literature by using green route for intracellular biosynthesis of selenium nanoparticle using Lactobacillus pentosus ADET MW861694 and by analyzing the antibacterial potential of the fabricated nanoparticles against some food pathogens. This study uses new estimation methodology for characterization of the fabricated selenium nanoparticles. INTRODUCTIONSpoilage of food through the activities of microorganisms which resulted in a reduction in shell-life which can result in an increase in food-borne infection and intoxication and probably shortage of food is a serious global concern. The outbreak of food-borne infection and intoxication possess a serious health threat to the populace all over the world. The search for newer antimicrobials with dual functions to serve as an antagonistic agent and nutrient enrichment and fortification in the food industry resulted in the emergence of nanotechnology in this

A review on synthesis and antibacterial potential of bio-selenium nanoparticles in the food industry

“…Bio-SeNPs synthesized by Lactobacillus pentosus ADET MW861694 were used to control foodborne pathogens such as Salmonella enterica subsp. arizonae , E. coli , S. enterica serotype Typhimurium, and S. aureus ( Christianah Adebayo-Tayo et al, 2021 ). Similarly, Bio-SeNPs synthesized by Lactobacillus sporogenes were used to inhibit S. aureus and E. coli ( Kaur et al, 2018 ).…”

Selenium Nanoparticles: Green Synthesis and Biomedical Application

“…An interesting object for intracellular selenium nanoparticle production are the Gramnegative bacteria Delftia sp., known for their unique metabolic abilities to transform various pollutants; in this case, the reduction of selenite to SeNPs did not have harmful effects on the cell structure [51]. Moreover, selenium nanoparticles synthesized intracellularly using Lactobacillus pentosus were agglomerated in the surrounding medium [52]. Khoei et al showed that after the synthesis by Burkholderia fungorum, SeNPs were present both intracellularly and extracellularly.…”

Inhibitory effects of glutathione peroxidase on microbial spoilage of crayfish (Procambarus clarkii) during refrigerated storage

“…In contrast, biosynthetic SeNPs derived from plants, bacteria and fungi cultured in selenium-fortified substrates have a better safety profile ( Han et al, 2021 ). The synthesized SeNPs by dos Santos Souza et al (2022) from extracts of three plants, onion ( Allium cepa ), acerola ( Malpighia emarginata ) and boldo ( Gymnanthemum amygdalinum ) inhibit Gram-positive bacteria, while SeNPs biosynthesized in cells of Lactobacillus pentosus ADEB had strong antagonistic activity against the Gram-negative bacteria Salmonella arizonae ( Adebayo-Tayo, Yusuf, & Alao, 2021 ). Meanwhile, due to its favorable biological activity and safety profile, organic selenium has also attracted much research attention recently on its bacteriostatic ability.…”