Antibacterial Activity of Isolated Endophytic Fungi From Rauvolfia Serpentina (L.) Benth. Ex Kurz

Abstract

Objective: The aim of the present study was to isolate the endophytic fungi from medicinal plant Rauvolfia serpentina (L.) Benth. ex Kurz. (Family Apocynaceae) and observed their antibacterial activity against bacteria as well as the molecular characterization of most potent fungal strain. Methods: Collection and isolation of endophytic fungi from different parts (root, shoot, leaves) of Rauvolfia serpentina plant. Screening of endophytic fungi for antibacterial activity was scrutinised against six bacteria viz. Bacillus subtilis, Enterococcus sp., Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium and Streptococcus pyogenes by using Agar well diffusion method. For molecular sequencing of potent fungi, the DNA was extracted, quantified and amplified by using two oligonucleotide primers ITS4 and ITS6 in PCR.Results: A total seven endophytic fungi Aspergillus niger, Penicillium citrinum, Cladosporium sp., Curvularia lunata, Aspergillus sp., Alternaria sp. and Aspergillus fumigatus were isolated from different parts of Rauvolfia serpentina and fungal strain Penicillium citrinum was shown the maximum zone of inhibition against Bacillus subtilis (23.0±0.12 mm), Escherichia coli (19.9±0.16 mm), Streptococcus pyrogens (19.2±0.59 mm), Enterococcus sp., (17.2±0.08 mm), Klebsiella pneumoniae (18.9±0.16 mm) and Salmonella typhimurium (15.1±0.16 mm). The molecular sequencing of the potent fungi was done by primers (ITS4 and ITS6) which showed strong specificity with fungal DNA and the percentages of identical matches of ITS4 and ITS6 DNA sequences in the GeneBank database (NCBI) were determined to 98 %.Conclusion: In the present study, the endophytic fungal strain Penicillium citrinumshowed the potential source of antibacterial bioactive compounds and molecular sequencing of this fungus helps in future to determine the various metabolic pathways that are responsible for the production of such type of novel compounds.

Antioxidative and Antibacterial Potentials of Fungal Endophytes From Justicia Wynaadensis Heyne: An Ethnomedicinal Rain Forest Species of Western Ghats

“…In consistent with this study Alternaria sp. isolated from medicinal plant Rauvolfia serpentina showed a zone of inhibition against K. pneumoniae (17.6±0.58 mm) and E. coli (05.7±00.43 mm) [35]. All the isolated strains except F. oxysporum formed inhibition zone against E. coli at 250 µg concentrations.…”

Synthesis of Linear and cyclic peptides analogues of Longicalycinin A and evaluation of toxicity effects on cancerous cells HepG2 and HT-29

“…We designed different amino acids for each position of diversities R1, R2, R3, R4 and R5 for the synthesis of eighteen Longicalycinin A analogues, based on conserving lipophilic characteristic of peptides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Thus, we chose Ala, Val and Gly for position R1, Phe and Ala for position R2, Pro for position R3, Tyr (t-Bu), Ser(t-Bu) and Thr(t-Bu) for position R4 and Gly, Leu and Phe for position R5 in the first category.…”

“…Cancers are similar in some ways, but different in growth and spread routes. 2 Normal cells are divided in a regular routine order. They die when become damaged and new cells take their place.…”

Caesium carbonate promoted regioselective O-functionalization of 4,6-diphenylpyrimidin-2(1H)-ones under mild conditions and mechanistic insight

“…1). [1][2][3][4] A range of substituents/functional groups have been explored at different positions of pyrimidinones to evaluate their impact on the activity. [5][6][7][8][9][10][11] In particular, specic attention has been paid to the selective N/O-alkylation of the pyrimidinones.…”