Antibacterial activity of Lactobacillus plantarum UG1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin UG1

Enan, Gamal; El-Essawy, A.A.; Uyttendaele, Mieke; Debevere, Johan

doi:10.1016/0168-1605(96)00947-6

Cited by 144 publications

(109 citation statements)

References 54 publications

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“…A combination of K2HPO4 and KH2PO4 (2.0g/L and 2.0g/L) resulted in the reduction of bacteriocin AMA-K by 50% (Table 2). In the case of plantaricin UG1, 7.0g/L K2HPO4 resulted in increased activity (13). Different concentrations of K2HPO4 and KH2PO4 did not significantly affect production of bacteriocin ST712BZ, produced by L. pentosus ST712BZ (40).…”

Section: Effect Of Inorganic Salts and Organic Compounds On Adsorptiomentioning

confidence: 96%

“…Apart from studies conducted on the effect of nitrogen and carbon sources on the production of plantaricin ST31 (37), plantaricin 423 (46), plantaricin UG1 (13), plantaricin KW30 (19), plantaricin-149 (18), plantaricin S (16), plantaricin ST13BR (44) and plantaricin A (8), little is known about the growth conditions required for optimal production of these bacteriocins. Studies conducted on bacteriocins from other lactic acid bacteria, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Bacteriocin production by Lactobacillus plantarum AMA-K isolated from Amasi, a Zimbabwean fermented milk product and study of the adsorption of bacteriocin AMA-K to Listeria sp.

Todorov¹

2008

Braz. J. Microbiol.

105

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Bacteriocin AMA-K produced by Lactobacillus plantarum AMA-K inhibits the growth of Enterococcus spp., Escherichia coli, Klebsiella pneumoniae and Listeria spp. Growth of strain AMA-K in BHI, M17, soy milk and molasses was similar to growth in MRS. The effect of organic nitrogen sources, carbohydrates, glycerol, K2HPO4 and KH2PO4, MgSO4, MnSO4, tri-ammonium citrate, Tween 80, vitamins and initial pH on bacteriocin AMA-K was determined. The mode of action of bacteriocin AMA-K was studied. The effect of bacteriocin AMA-K to actively growing Listeria innocua LMG13568, L. ivanovii subsp. ivanovii ATCC19119 and L. monocytogenes ScottA was determined. Adsorption of bacteriocin AMA-K to target cells at different temperatures, pH and in presence of Tween 20, Tween 80, ascorbic acid, potassium sorbate, sodium nitrate and sodium chloride were studied. Bacteriocin AMA-K shares high homology to pediocin PA-1.

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Section: Effect Of Inorganic Salts and Organic Compounds On Adsorptiomentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Bacteriocin production by Lactobacillus plantarum AMA-K isolated from Amasi, a Zimbabwean fermented milk product and study of the adsorption of bacteriocin AMA-K to Listeria sp.

Todorov¹

2008

Braz. J. Microbiol.

105

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“…The physiological properties of bacteria isolated from meat fermentations, such as bacteriocins (Meisel et al 1989 ;Schillinger and Lücke 1989 ;Farber et al 1993 ;Vignolo et al 1993 ;Giraffa et al 1994 ;Rekhif et al 1995 ;Enan et al 1996), lipolysis (Garcia et al 1992 ;Ortiz de Apodaca et al 1993 ;Johansson et al 1994), proteolysis (Grazia et al 1990 ;Ortiz de Apodaca et al 1993 ;Johansson et al 1994), nitrite and nitrate reductases (Wolf and Hammes 1988 ;Buckenhüskes 1993 ;Mares et al 1994) have been studied in detail.…”

Section: It)mentioning

confidence: 99%

Physiological and molecular techniques for the study of bacterial community development in sausage fermentation

et al. 1998

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The development of the microbial community involved in the production process of Italian dry sausage was investigated using physiological analysis and molecular techniques for strain typing and taxonomical identification. A cycle of sausage production was followed collecting samples during the 2 months of ripening process. Microbiological analysis allowed the identification of the main bacterial groups responsible for the fermentation process as lactobacilli and coagulase‐negative staphylococci. The use of a polymerase chain reaction‐based technique of strain typing, RAPD fingerprinting, demonstrated that the environmental parameters interact to select a limited number of strains that dominate the fermentation process. The staphylococcal populations were characterized for their physiological properties and the two dominant strains were identified as Staphylococcus xylosus and Staph. sciuri. The use of 16S rDNA sequencing allowed the definition of the taxonomical position of the two dominant strains of lactic acid bacteria, as belonging to Lactobacillus sake and Lact. plantarum.

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“…Several methods have been reported for the purification of plantaricins: (a) anion-exchange chromatography (DEAESephadex A-25) and reverse-phase HPLC (9); (b) ammonium sulfate precipitation (80%), cation-exchange chromatography (SP Sepharose fast-flow cation exchange column), hydrophobic interaction chromatography (phenyl-Sepharose CL-4B column) and C 2 /C 18 reverse-phase chromatography (8); (c) ammonium sulfate precipitation (55%), hydrophobic interaction (C 8 ), cation exchange chromatography Mono S cation-exchange column (Pharmacia, Biotech) (6); (d) ammonium sulfate precipitation (40%) and cation exchange -SP-Sepharose (1); (e) ammonium sulfate precipitation (60%), cation-exchange chromatography, hydrophobic interaction chromatography (11); (f) ammonium sulfate precipitation, cation exchange (S-Sepharose), reversedstationary-phase (octyl-sepharose -CL-4B), stationary-phase C2/C18 chromatography (5,10) and (g) ammonium sulfate precipitation (60%), dialysis, filtration (Amicon, 0.45µm Millipore) and ultrafiltration (4). A rapid and two-step procedure suitable for both small-and large-scale purification of pediocin-like bacteriocins and other cationic peptides have been reported (15).…”

Section: Introductionmentioning

confidence: 99%

Comparison of two methods for purification of plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31

Todorov¹,

Vaz-Velho

Gibbs

2004

Braz. J. Microbiol.

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Two methods of purification of the plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31 are used in this study -the method of ammonium sulfate precipitation, Sep-pack C 18 cartridge and reverse-phase HPLC chromatography on C 18 Nucleosil column, and the method of direct purification by cation exchange SP Sepharose Fast Flow column Amersham (Pharmacia Biotech). The purity of the products from the two experimental protocols are examined for their molecular weight, aminoacid composition and sequence. Comparison of results show that the plantaricins purified with the two methods are identical. Both methods may be used to purify plantaricin ST31. Comparison of the yield in the purification protocols is 0.8% in the HPLC experimental protocol and 5.9% in the cation-exchange chromatography method.

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Antibacterial activity of Lactobacillus plantarum UG1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin UG1

Cited by 144 publications

References 54 publications

Bacteriocin production by Lactobacillus plantarum AMA-K isolated from Amasi, a Zimbabwean fermented milk product and study of the adsorption of bacteriocin AMA-K to Listeria sp.

Bacteriocin production by Lactobacillus plantarum AMA-K isolated from Amasi, a Zimbabwean fermented milk product and study of the adsorption of bacteriocin AMA-K to Listeria sp.

Physiological and molecular techniques for the study of bacterial community development in sausage fermentation

Comparison of two methods for purification of plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31

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