Antibacterial activity of Lamiaceae plant extracts in clinical isolates of multidrug-resistant bacteria

Assis, Felipe V De; Siqueira, Flávia L; Gonçalves, Isabela E; Lacerda, Rafael P.; Nascimento, Rafaela A; Araújo, Sthéfane Guimarães; Andrade, Jéssica Tauany; Herrera, Karina Marjorie Silva; Lima, Luciana Alves Rodrigues dos Santos; Ferreira, Jaqueline Ms

doi:10.1590/0001-3765201820160870

Cited by 21 publications

(15 citation statements)

References 24 publications

(15 reference statements)

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“…Regarding the activity of extract against multidrugresistant strains, Assis et al (2018) evaluated the ethanol extract of three Lamiaceae species against Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa clinical isolates with multidrugresistant profile. They identified that the P. barbatus extract was the most effectiveness, exhibiting MIC of 2000, 1000, 1000 and 500 µg.mL -1 respectively, however, they did not identify the extract composition.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

et al. 2022

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Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory.

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Section: Discussionmentioning

confidence: 99%

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

et al. 2022

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“…28 Many of these diseases are caused by bacterial infections and numerous studies have reported antibacterial properties for species of this genus. [41][42][43] However, many of these studies either do not quantify the activity, or report low to moderate activity for the extracts and oils of these plants.…”

Section: Discussionmentioning

confidence: 99%

Westringa fruticosa (Willd.) Druce Leaf and Flower Extracts Lack Antibacterial Activity and are Non-toxic in vitro

Chikowe¹,

Mpala²,

Cock³

2019

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Introduction: Closely related plant species often share similar secondary metabolites and bioactivities and are therefore good targets for bioactivity testing when one or more species within a family are known to possess therapeutic properties. The family Laminaceae has a long history of medicinal usage globally. Many species are known to have therapeutic properties, several species of which have well established antibacterial bioactivities. Methods: The ability of Westringa fruticosa leaf and flower extracts to inhibit the growth of a panel of bacterial and fungal pathogens was investigated by disc diffusion assay. Toxicity was examined using the Artemia franciscana nauplii bioassay. Results: W. fruticosa leaf methanolic and aqueous extracts were both completely ineffective at inhibiting the growth of gram-positive and gram-negative panels of bacteria, as well as fungi. The extracts were non-toxic or of low toxicity in the Artemia bioassay following 24 h exposure. Conclusion: Despite the taxonomic relationship to several bioactive Laminaceae spp., W. fruticosa leaf and flower extracts were completely ineffective bacterial and fungal growth inhibitors. However, these extracts may have other therapeutic properties and testing against protozoa, virus and tumour cells is required.

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“…Several studies conducted with plant extracts and essential oils have demonstrated efficacy of some compounds with antimicrobial activities against bacteria [29][30][31] and fungi. [32][33][34] These compounds are secondary metabolites of plants with variations in their structure and chemical composition resulting in different antimicrobial effects.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, others considered MIC values ≤ 1 mg/ml as satisfactory and promising. 43,30 Considering this parameter, the inhibitory effects results obtained with crude extracts from P. cattleianum, R. officinalis and O. vulgare were characterized as satisfactory against S. aureus. However, none of the plant extracts showed satisfactory results against A. parasiticus, according to the recommendations of other studies.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial effects of Extracts from Leaves of Medicinal Herbs and Native Brazilian Plants

et al. 2018

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The objective of this study was to evaluate the antimicrobial effects of crude and lyophilized extracts of leaves from sweet passion fruit (Passiflora alata), araçá (Psidium cattleianum), rosemary (Rosamrinus officinalis) and oregano (Origanum vulgare) on planktonic cells of Staphylococcus aureus and Aspergillus parasiticus. Sweet passion fruit showed no inhibitory effect against the micro-organisms tested. However, crude and lyophilized extracts from Araçá had the highest (P < 0.05) antimicrobial activity against S. aureus, with minimum inhibitory concentrations (MIC) of 0.39 and 0.35 mg/ml, respectively. MIC values against S. aureus for lyophilized extracts from rosemary and crude extracts from oregano were 0.57 and 0.65 mg/ml, respectively. None of the extracts demonstrated effective results against A. parasiticus, although araçá and oregano extracts had the lowest (P < 0.05) MIC values when compared with the other extracts. This preliminary screening study indicated that araçá, rosemary and oregano are interesting alternatives as antimicrobial agents in food substrates, although further studies are needed to develop commercial formulations based on field trials.

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Antibacterial activity of Lamiaceae plant extracts in clinical isolates of multidrug-resistant bacteria

Cited by 21 publications

References 24 publications

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

Westringa fruticosa (Willd.) Druce Leaf and Flower Extracts Lack Antibacterial Activity and are Non-toxic in vitro

In Vitro Antimicrobial effects of Extracts from Leaves of Medicinal Herbs and Native Brazilian Plants

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