Antibacterial Activity of Manganese Dioxide Nanosheets by ROS-Mediated Pathways and Destroying Membrane Integrity

Abstract

Manganese dioxide (MnO2) nanosheets have shown exciting potential in nanomedicine because of their ultrathin thickness, large surface area, high near-infrared (NIR) absorbance and good biocompatibility. However, the effect of MnO2 nanosheets on bacteria is still unclear. In this study, MnO2 nanosheets were shown for the first time to possess highly efficient antibacterial activity by using Salmonella as a model pathogen. The growth curve and surface plate assay uncovered that 125 μg/mL MnO2 nanosheets could kill 99.2% of Salmonella, which was further verified by fluorescence-based live/dead staining measurement. Mechanism analysis indicated that MnO2 nanosheet treatment could dramatically induce reactive oxygen species production, increase ATPase activity and cause the leakage of electrolytes and protein contents, leading to bacterial death. These results uncover the previously undefined role of MnO2 nanosheets and provide novel strategies for developing antimicrobial agents.

Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles

“…CuNPs are also well known to be highly cytotoxic by the release of ions and the production of ROS [59]. Additionally, other MONPs, such as ZnO [60], TiO 2 [61], and MnO [62], have been proven to exhibit bactericidal activity via their ability to stimulate the cell to produce ROS (Figure 1). In addition to the disruption of bacterial membranes, NPs have been demonstrated to disrupt biofilm formation.…”

Oxygen Self‐Sufficient Nanoplatform for Enhanced and Selective Antibacterial Photodynamic Therapy against Anaerobe‐Induced Periodontal Disease

“…In the present study, F@Ce6‐M could decompose H 2 O 2 into O 2 , thus boosting the generation of ROS. Moreover, it has been suggested that MnO 2 nanostructure also have highly effective antibacterial activity, [ 43 ] because of the production of ROS, the increase of ATPase activity, the leakage of protein contents and electrolytes. On the basis of single bacteria level experiments, the F@Ce6‐M NCs exerted its enhanced antibacterial effect on the disruption of the biofilm by increasing local oxygen tension.…”

The chemodynamic antibacterial effect of MnOX nanosheet decorated silicon nanowire arrays

“…The energy difference between the two peaks is 11.7 eV, indicating that Mn 4+ in MnO X is the main oxidation state of Mn 41,44 . MnO 2 can induce the production of Please do not adjust margins Please do not adjust margins ROS, increase the activity of ATPase and cause the leakage of electrolyte and protein content, leading to the death of bacteria 45 .…”