Antibacterial activity of medicinal plant extracts

Abstract

The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus and Enterococcus sp. The methanolic extract of Caryophyllus aromaticus presented the highest anti-S. aureus activity and was effective against all bacterial strains tested. 3815-3744. E-mail: ary@ibb.unesp.br Further acquaintance with different ethnic groups has contributed to the development of research on natural products, to the increase in knowledge about the close relationship between the chemical structure of a certain compound and its biological properties, and to the understanding of the animal/ insect-plant interrelation (8). For these reasons, medicinal plants are important substances for the study of their traditional uses through the verification of pharmacological effects and can be natural composite sources that act as new anti-infectious agents.The present study aimed at evaluating the in vitro antimicrobial activity of plant (Allium sativum, Zingiber officinale, Caryophyllus aromaticus, Cymbopogon citratus, Mikania glomerata and Psidium guajava) extracts against Gram-positive and Gram-negative bacterial strains isolated from human infections. The determined plant parts (200g) were ground, extracted with 70% methanol and filtered after 48hs. The plant residue was re-extracted by adding 70% methanol and filtered again after 48hs. Such procedure was repeated every 72hs, completing three filtration processes. The filtrate was concentrated on a rotary evaporator at 45ºC for methanol elimination, and the extracts were kept in sterile bottles under refrigerated conditions until use. The dry weight of the extracts was obtained by allowing the solvent to evaporate and was used to determine concentration in mg/mL. (Methodology based on Betoni et al. (3); Table 1).Microbial susceptibility assays using the agar dilution (Mueller-Hinton Agar) method (%v/v and corresponding mg/ mL values) and the Minimal Inhibitory Concentration (MIC) were carried out for fifteen Salmonella Typhimurium, S. aureus, Enterococcus sp and E. coli strains plus one ATCC strain of each bacterium. Overnight cultures (37ºC) in Brain Heart

Mastite em pequenos ruminantes no Brasil

“…Mitidiero (2002) demonstrou que esta terapia alternativa permite manter a sanidade do rebanho em padrões semelhantes aos da alopatia, constituindo-se assim, em uma opção viável para os produtores de leite. Estudos in vitro visando à determinação do potencial antimicrobiano de plantas pertencentes à flora brasileira têm demonstrando resultados satisfatórios (Granato et al 2005, Oliveira et al 2007, Ushimaru et al 2007), porém observase a descontinuidade destes estudos e uma fragmentação dos resultados, não permitindo muitos avanços na área.…”

Avaliação das atividades antibacteriana, tripanocida e citotóxica do extrato hidroalcóolico das raízes de Tradescantia sillamontana Matuda (Veludo Branco) (Commelinaceae)

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