Antibacterial Activity of Methyl Aminolevulinate Photodynamic Therapy in the Treatment of a Cutaneous Ulcer

Devirgiliis, Valeria; Panasiti, Vincenzo; Fioriti, D.; Anzivino, Elena; Bellizzi, Anna; Cimillo, Marco; Curzio, Michela; Melis, Luca; Roberti, Vincenzo; Gobbi, Silvia; Lieto, Piergiorgio; Richetta, Antonio Giovanni; Calvieri, Stefano; Chiarini, F.; Nicosia, R; Pietropaolo, Valeria

doi:10.1177/039463201102400326

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…It has been reported that high TNF-α level inhibit angiogenesis (Fajardo et al, 1992) and wound repair is promoted due to inhibition of TNF-α (Mori et al, 2002). PDT can intervene by stimulating acute inflammation, contributing to changes in the physiological processes in chronic wounds, regardless of whether they are infected, promoting healing (Devirgiliis et al, 2011;Cappugi et al, 2014;Lei et al, 2015). Recent studies indicate that PDT induces a localized acute inflammatory response, leading to activation of the immune system (Anzengruber et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of ALA-PDT on the Healing of Mouse Skin Wounds Infected With Pseudomonas aeruginosa and Its Related Mechanisms

Yang

Tan

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Photodynamic therapy (PDT) is a promising new method to eliminate microbial infection and promote wound healing. Its effectiveness has been confirmed by some studies; however, the mechanisms of PDT in wound healing remain obscure. We used mouse skin wounds infected with Pseudomonas aeruginosa as a research object to explore the therapeutic effects and mechanisms of 5-aminolevulinic acid photodynamic therapy (ALA-PDT). ALA-PDT treatment significantly reduced the load of P. aeruginosa in the wound and surrounding tissues and promoted the healing of skin wounds in mice. Hematoxylin-eosin (HE) and Sirius red staining showed that ALA-PDT promoted granulation tissue formation, angiogenesis, and collagen regeneration and remodeling. After ALA-PDT treatment, the expression of inflammatory factors (TNF-α and IL-1β) first increased and then decreased, while the secretion of growth factors (TGF-β-1 and VEGF) increased gradually after treatment. Furthermore, ALA-PDT affected the polarization state of macrophages, activating and promoting macrophages from an M1 to an M2 phenotype. In conclusion, ALA-PDT can not only kill bacteria but also promote wound healing by regulating inflammatory factors, collagen remodeling and macrophages. This study further clarifies the mechanism of PDT in the healing of infectious skin wounds and provides further experimental evidence for its clinical treatment of skin wounds infected by P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Effects of ALA-PDT on the Healing of Mouse Skin Wounds Infected With Pseudomonas aeruginosa and Its Related Mechanisms

Yang

Tan

Zhang

et al. 2020

Front. Cell Dev. Biol.

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“…There are two main causes that prevent the healing of ulcers: a vascular insufficiency that causes a lack of oxygen and nutrients , and the almost constant presence of a bacterial biofilm that “paralyses” the fibroblasts and the repairing mechanism of epidermal cells . Recent studies showed the increased fibroblastic activity of photodynamic therapy (PDT) with the production of neocollagen, the modulation of matrix metallopeptidase MMP1 e MMP3, the production of both vascular endothelial growth factor and keratinocyte growth factor , and its antibacterial effect . Considering these studies and according to our experience on how PDT can heal carcinomatous ulcer lesions without or with very little scarring, we report the successful off‐label use of PDT for the treatment of CLUs.…”

Section: Introductionmentioning

confidence: 99%

Effective use of photodynamic therapy on the treatment of chronic leg ulcers: a pilot study

Mavilia¹,

Cantarella²,

Cicero

et al. 2015

Dermatologic Therapy

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“…The possibility of using MAL as a precursor of PP-IX was also considered by the Devirgiliis group to treat a patient with chronic venous ulceration infected by the two Grampositive bacteria S. aureus and E. faecalis and not responsive to conventional treatments [86]. The lesion was treated with a cream containing MAL and after 3 h was irradiated with a LED light at 630 nm (total light dose of 37 J/cm 2 ).…”

Section: Figure 10mentioning

confidence: 99%

The Role of Porphyrinoid Photosensitizers for Skin Wound Healing

Vallejo

Moura

Gomes

et al. 2021

IJMS

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Microorganisms, usually bacteria and fungi, grow and spread in skin wounds, causing infections. These infections trigger the immune system and cause inflammation and tissue damage within the skin or wound, slowing down the healing process. The use of photodynamic therapy (PDT) to eradicate microorganisms has been regarded as a promising alternative to anti-infective therapies, such as those based on antibiotics, and more recently, is being considered for skin wound-healing, namely for infected wounds. Among the several molecules exploited as photosensitizers (PS), porphyrinoids exhibit suitable features for achieving those goals efficiently. The capability that these macrocycles display to generate reactive oxygen species (ROS) gives a significant contribution to the regenerative process. ROS are responsible for avoiding the development of infections by inactivating microorganisms such as bacteria but also by promoting cell proliferation through the activation of stem cells which regulates inflammatory factors and collagen remodeling. The PS can act solo or combined with several materials, such as polymers, hydrogels, nanotubes, or metal-organic frameworks (MOF), keeping both the microbial photoinactivation and healing/regenerative processes’ effectiveness. This review highlights the developments on the combination of PDT approach and skin wound healing using natural and synthetic porphyrinoids, such as porphyrins, chlorins and phthalocyanines, as PS, as well as the prodrug 5-aminolevulinic acid (5-ALA), the natural precursor of protoporphyrin-IX (PP-IX).

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Antibacterial Activity of Methyl Aminolevulinate Photodynamic Therapy in the Treatment of a Cutaneous Ulcer

Cited by 12 publications

References 9 publications

Effects of ALA-PDT on the Healing of Mouse Skin Wounds Infected With Pseudomonas aeruginosa and Its Related Mechanisms

Effects of ALA-PDT on the Healing of Mouse Skin Wounds Infected With Pseudomonas aeruginosa and Its Related Mechanisms

Effective use of photodynamic therapy on the treatment of chronic leg ulcers: a pilot study

The Role of Porphyrinoid Photosensitizers for Skin Wound Healing

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