Antibacterial Activity of Punica granatum (Pomegranate) Fruit Peel Extract against Pathogenic and Drug Resistance Bacterial Strains

Chaudhary, Alka; Rahul, Siddarth N

doi:10.20546/ijcmas.2017.612.437

Cited by 9 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antimicrobial effect of ethanolic and methanolic pomegranate peel extracts reported in this study was previously reported by several authors [28,[36][37][38][39]. With respect to G + bacteria, our study is in line with others that reported the superiority of the methanolic pomegranate peel extract over the ethanolic one [36][37][38][39].…”

Section: Discussionsupporting

confidence: 92%

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Hanafy¹,

El-Shafea²,

Saleh

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels. The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. Results The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit peels (orange, pomegranate, and banana), against 4 Gram-positive (G+), 3 Gram-negative bacteria (G−), and 2 fungal strains revealed that both pomegranate peel extracts exhibited significantly higher inhibitory effect on all tested G+ bacteria. Methanolic extract of pomegranate peel gave higher activity than the ethanolic one against G+ and G− bacteria except for S. typhimurium. Against A. flavus and A. niger, both pomegranate and orange extracts showed activity ranging between 65 and 100% more than the positive control. The ethanolic extracts of all tested peels showed a considerable capacity of antioxidant compounds compared to the methanolic extracts. The highest antioxidant capacity was found for ethanolic and methanolic extracts of pomegranate, 66.870 and 56.262 mg/ml, respectively. Generally, the concentration of total phenolic compounds was higher than that of total flavonoids followed by tannins. The highest readings of all tested constituents were reported for pomegranate extracts followed by orange and then banana. The total phenolic content, total flavonoids, and tannins were proportional to antioxidant values. GC-MS of pomegranate peel extracts identified 23 compounds in the methanolic extract versus 31 compounds in the ethanolic one. These components were identified based on their retention times and mass spectral fragmentation pattern. 5-hydroxymethylfufural (HMF) represented the major component in both methanolic and ethanolic extracts with peak area percentage of 65.78% and 48.43%, respectively. Conclusions The results showed negative effect of methanolic and ethanolic extracts of pomegranate on G+ and G− bacteria and two fungal pathogenic strains. The phytochemical analysis regarded these results to the high content of phenols, flavonoids, and tannins. GC-MS chromatogram identified many compounds known to be effective as antioxidants and antibacterial and antifungal agents. These indications show that pomegranate peel may be a superior natural food-preserver, but further studies about the suitable formulation, dosage, and possible side-effects are still needed.

show abstract

Section: Discussionsupporting

confidence: 92%

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Hanafy¹,

El-Shafea²,

Saleh

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…ZnO-NPs synthesis from diverse resources such as Ziziphus nummularia leaf extract [5], starch [18], gum tragacanth [13], Catharanthus roseus leaf extract [19], honey [7], brown microalga [20] and seaweed [21] have been described previously. The properties of ZnO-NPs have been investigated in a wide range of targeted applications including electrical property [22], antibacterial activity [23], antifungal activity [14], catalytic activity [15] and cosmetic industry [21].…”

Section: Introductionmentioning

confidence: 99%

“…Punica granatum fruit (P. granatum F.) or pomegranate has been described as a power fruit, well known for its superior therapeutic properties and health benefits to consumers [23]. P. granatum F. peel makes up about one third of the fruit and remains as a byproduct after consumption.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and antibacterial activities of plant-mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract

Sukri

Shameli

Wong

et al. 2019

Journal of Molecular Structure

179

View full text Add to dashboard Cite

The unique properties of zinc oxide nanoparticles (ZnO-NPs) produced using plant extract make them attractive for use in medical as well as industrial applications, and it is necessary to develop environmentally friendly methods for their synthesis. This can be accomplished by replacing the traditional chemical compounds for the reduction of the zinc ions to ZnO-NPs during synthesis with natural plant extracts. Here, the biosynthesis of ZnO-NPs using Punica granatum (P. granatum) fruit peels extract was investigated as the reducing and stabilizing agent. The P. granatum/ZnO-NPs with spherical and hexagonal shapes were biosynthesized at different annealing temperatures. The X-ray diffraction analysis confirmed the synthesis of highly pure ZnO-NPs with increasing crystallinity in higher annealing temperatures. The ZnO-NPs displayed characteristic absorption peaks between 370 and 378 nm in the UV evis spectra. Transmission electron microscopy (TEM) imaging showed the formation of mostly spherical and hexagonal-shaped ZnO-NPs in the mean size of 32.98 nm and 81.84 nm at 600 C and 700 C respectively. According to FTIR spectrum, strong absorption bands in the range of 462e487 cm À1 corresponding to ZneO bond stretching can be seen. Antibacterial activities of P. granatum/ZnO-NPs against Escherichia coli (E. coli) and Enterococcus faecalis (E. faecalis) were investigated and compared. Results obtained show that smaller-sized P. granatum/ZnO-NPs are more effective in inhibiting growth of both bacteria. In addition, cytotoxicity assays were performed for P. granatum/ZnO-NPs against human colon normal and cancerous cells. P. granatum/ZnO-NPs exhibited similar killing activities of both cell lines at the concentration of !31.25 mg/mL. The biosynthesized ZnO-NPs could offer potential applications in biomedical field.

show abstract

“…The research reports demonstrated its anti-cancer, anti-inflammatory, anti-atherogenic, antidiabetes, hepatoprotective and antioxidant activity [5][6][7] . It's antimicrobial activity has also been reported [8,9] . The present investigation was aimed to validate the efficacy of anti-tuberculosis activity of P. granatum and identification of lead molecules through in silico method.…”

mentioning

confidence: 84%

Anti-Tuberculosis Activity in Punica granatum: In silico Validation and Identification of Lead Molecules

Basheera¹,

Sivan²,

Kamalan³

2021

ijps

View full text Add to dashboard Cite

Basheera et al.: In silico Identification of Lead Molecules in Punica granatumDiscovery of novel drug against tuberculosis is inevitable since resistant bacterial strains are evolved against existing drugs and rapidly active single drug in short period is necessary for effective treatment. When compared to synthetic drugs, natural drugs particularly phytochemicals induce less side effects and long term stability. In Indian traditional medicine, several plant species have been used for treating tuberculosis and each plant species contains a plethora of phytochemicals. The efficacy of such treatment system and identification of the phytochemical with drug activity from plants has been seldom investigated scientifically. In this backdrop, the authors have validated the efficacy of anti-tuberculosis activity and identified lead molecule from a widely used plant species against several disease including tuberculosis, viz Punica granatum L. The four promising target proteins viz mycolyltransferase antigen protein 85C involved in cord factor synthesis, filamentous temperature sensitive protein Z involved in bacterial cell division, pantothenate kinase involved in co-enzyme A pathway and decaprenylphosphoryl β-D-ribofuranose-2 epimerase involved in the synthesis of virulent factor arabinan were docked with 243 phytochemicals derived from Punica granatum. The docked molecules having binding energy ≤-6 kcal/ mol were considered as active/hit molecules. The docked results showed that out of 243 phytochemicals, 126 have inhibitory activity on all selected target proteins. Further docking study using Glide and absorption, distribution, metabolism, excretion and toxicity studies revealed that the compound derived from the flowers, pomegranate can be recommended as the best lead compound against tuberculosis.

show abstract

Antibacterial Activity of Punica granatum (Pomegranate) Fruit Peel Extract against Pathogenic and Drug Resistance Bacterial Strains

Cited by 9 publications

References 10 publications

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Cytotoxicity and antibacterial activities of plant-mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract

Anti-Tuberculosis Activity in Punica granatum: In silico Validation and Identification of Lead Molecules

Contact Info

Product

Resources

About