Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

Gevorgyan, Susanna; Schubert, Robin; Yeranosyan, Mkrtich A.; Gabrielyan, Lilit; Trchоunian, Armen; Lorenzen, Kristina; Trchounian, Karen

doi:10.1186/s13568-021-01213-9

Cited by 20 publications

(9 citation statements)

References 32 publications

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“…To note that the IC50 value of sample A was not determined for S. aureus because the AgNPs were prepared using 300 µ M of AgNO3, tested starting from 46 µ M, and the Gram-positive strain are greatly resistant to AgNPs compared to E. coli. This finding has been previously reported for other Ag-based compounds [39,40] and it could be ascribed to a different interaction of the NPs with the bacterial cell wall of Gram positive and Gram-negative bacteria. S1).…”

Section: Antibacterial Assaysupporting

confidence: 84%

Effectiveness of Snail Slime in the Green Synthesis of Silver Nanoparticles

et al. 2022

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The development of green, low cost and sustainable synthetic routes to produce metal nanoparticles is of outmost importance, as these materials fulfill large scale applications in a number of different areas. Herein, snail slime extracted from Helix Aspersa snails was successfully employed both as bio-reducing agent of silver nitrate and as bio-stabilizer of the obtained nanoparticles. Several trials were carried out by varying temperature, the volume of snail slime and the silver nitrate concentration to find the best biogenic pathway to produce silver nanoparticles. The best results were obtained when the synthesis was performed at room temperature and neutral pH. UV–Visible Spectroscopy, SEM-TEM and FTIR were used for a detailed characterization of the nanoparticles. The obtained nanoparticles are spherical, with mean diameters measured from TEM images ranging from 15 to 30 nm and stable over time. The role of proteins and glycoproteins in the biogenic production of silver nanoparticles was elucidated. Infrared spectra clearly showed the presence of proteins all around the silver core. The macromolecular shell is also responsible of the effectiveness of the synthesized AgNPs to inhibit Gram positive and Gram negative bacterial growth.

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Section: Antibacterial Assaysupporting

confidence: 84%

Effectiveness of Snail Slime in the Green Synthesis of Silver Nanoparticles

et al. 2022

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“…The comparison between unfractionated, filtered, and isolated nanoparticles confirmed the antibacterial effectiveness of Growth data analysis showed that Gram-negative E. coli (Figure 6a, left) had a higher sensitivity than Gram-positive S. aureus (Figure 6a, right). Indeed, the cell wall structure plays a crucial role in the antibacterial activity of Ag-based compounds; it is well known that Gram-negative bacteria are more susceptible due to their narrower cellular walls compared to Gram-positive strains [62]. The purified Ag-PDA nanoparticles in fraction D completely inhibited E. coli when used at the highest dilution (1:10 with respect to the volume collected from the fractionation process) and with up to 24 h of incubation.…”

Section: Discussionmentioning

confidence: 99%

Synthesis Monitoring, Characterization and Cleanup of Ag-Polydopamine Nanoparticles Used as Antibacterial Agents with Field-Flow Fractionation

et al. 2022

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Advances in nanotechnology have opened up new horizons in nanomedicine through the synthesis of new composite nanomaterials able to tackle the growing drug resistance in bacterial strains. Among these, nanosilver antimicrobials sow promise for use in the treatment of bacterial infections. The use of polydopamine (PDA) as a biocompatible carrier for nanosilver is appealing; however, the synthesis and functionalization steps used to obtain Ag-PDA nanoparticles (NPs) are complex and require time-consuming cleanup processes. Post-synthesis treatment can also hinder the stability and applicability of the material, and dry, offline characterization is time-consuming and unrepresentative of real conditions. The optimization of Ag-PDA preparation and purification together with well-defined characterization are fundamental goals for the safe development of these new nanomaterials. In this paper, we show the use of field-flow fractionation with multi-angle light scattering and spectrophotometric detection to improve the synthesis and quality control of the production of Ag-PDA NPs. An ad hoc method was able to monitor particle growth in a TLC-like fashion; characterize the species obtained; and provide purified, isolated Ag-PDA nanoparticles, which proved to be biologically active as antibacterial agents, while achieving a short analysis time and being based on the use of green, cost-effective carriers such as water.

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“…Here, 5 mL AgNP sol and 3 mL of E. coli or S. aureus suspension at a concentration of 10 7 CFU/mL were added to the sterile Luria-Bertani (LB) medium (LB, 5 g yeast extract, 10 g NaCl, 10 g tryptone, and 1000 mL water). The bacteria were cultured and maintained in LB broth and then incubated at 37 °C at 180 rpm, while the bacterial cell proliferation was monitored at intervals according to absorption at 600 nm [ 46 ]. Finally, the growth curve plot was created using the optical density (OD) value vs. time.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized with the Peel Extract of Mango

et al. 2021

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The green synthesis of silver nanoparticles (AgNPs) from biological waste, as well as their excellent antibacterial properties, is currently attracting significant research attention. This study synthesized AgNPs from different mango peel extract concentrations while investigating their characteristics and antibacterial properties. The results showed that the AgNPs were irregular with rod-like, spherical shapes and were detected in a range of 25 nm to 75 nm. The AgNPs displayed antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), showing a more significant impact when synthesized with 0.20 g/mL of mango peel extract. Therefore, the antibacterial effect of different diluted AgNP concentrations on the growth kinetic curves of E. coli and S. aureus after synthesis with 0.20 g/mL mango peel extract was analyzed. The results indicated that the AgNP antibacterial activity was higher against S. aureus than against E. coli, while the AgNP IC50 in these two strains was approximately 1.557 mg/mL and 2.335 mg/L, respectively. This research provides new insights regarding the use of postharvest mango byproducts and the potential for developing additional AgNP composite antibacterial materials for fruit and vegetable preservation.

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Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

Cited by 20 publications

References 32 publications

Effectiveness of Snail Slime in the Green Synthesis of Silver Nanoparticles

Effectiveness of Snail Slime in the Green Synthesis of Silver Nanoparticles

Synthesis Monitoring, Characterization and Cleanup of Ag-Polydopamine Nanoparticles Used as Antibacterial Agents with Field-Flow Fractionation

Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized with the Peel Extract of Mango

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