Antibacterial activity of seed proteins of Robinia pseudoacacia

Talas-Oğraş, Tijen; Ipekci, Z.; Bajroviç, Kasım; Gözükırmızı, Nermin

doi:10.1016/j.fitote.2004.10.020

Cited by 22 publications

(13 citation statements)

References 18 publications

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“…Although the mechanism of action of the peptides has not yet been elucidated in detail, the presented data confirm the in vitro antibacterial activity of EuniSL against pathogenic bacteria. It has been proposed that the proteins with antibacterial action form a channel on cell membrane and the cell dies as a result of the out flowing of cellular contents, being this mechanism different from that of antibiotics (Talas‐Ogras et al. 2005).…”

Section: Discussionmentioning

confidence: 99%

Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity

Oliveira

Andrade

Santos-Magalhães

et al. 2008

Lett Appl Microbiol

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Aims: The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds. Methods and Results: The E. uniflora lectin (EuniSL) was isolated from the seed extract and purified by ion‐exchange chromatography in DEAE‐Sephadex with a purification factor of 11·68. The purified lectin showed a single band on denaturing electrophoresis, with a molecular mass of 67 kDa. EuniSL agglutinated rabbit and human erythrocytes with a higher specificity for rabbit erythrocytes. The haemagglutination was not inhibited by the tested carbohydrates but glycoproteins exerted a strong inhibitory action. The lectin proved to be thermo resistant with the highest stability at pH 6·5 and divalent ions did not affect its activity. EuniSL demonstrated a remarkable nonselective antibacterial activity. EuniSL strongly inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella sp. with a minimum inhibitory concentration (MIC) of 1·5 μg ml−1, and moderately inhibited the growth of Bacillus subtilis, Streptococcus sp. and Escherichia coli with a MIC of 16·5 μg ml−1. Conclusions: EuniSL was found to be effective against bacteria. Significance and Impact of the Study: The strong antibacterial activity of the studied lectin indicates a high potential for clinical microbiology and therapeutic applications.

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Section: Discussionmentioning

confidence: 99%

Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity

Oliveira

Andrade

Santos-Magalhães

et al. 2008

Lett Appl Microbiol

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“…The peptide inhibited the growth of the tested strains and S. aureus was found to be the most sensitive strain compared with others strains (Corynebacterium michiganense, Bacillus subtilis, Erwinia carotovora, Pseudomonas syringae, Xanthomonas campestris and Escherichia coli). 21 Poor data on the antioxidant activity of R. pseudoacacia extracts exists. The antioxidant activity of lyophilized extracts of acacia leaves was evaluated by the oxygen radical absorbance capacity (ORAC) assay.…”

mentioning

confidence: 99%

Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia

Marinaș

Oprea

Geană

et al. 2014

JSCS

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This study was aimed at investigating the antimicrobial and antioxidant activity of ethanol extracts obtained from the leaves, seeds and sheaths of Robinia pseudoacacia. Total phenolic content (TPC, Folin-Ciocalteu method), antioxidant activity (trolox equivalent antioxidant capacity (TEAC) assay) and antimicrobial activity (agar disk diffusion method and broth dilution method) of the vegetative and reproductive organs of R. pseudoacacia were determined. The highest content of polyphenols (expressed as gallic acid equivalents, GAE) was found in the extract of R. pseudoacacia leaves (266.7 μg GAE mL -1 extract) followed by the extract of the seeds (232.2 μg GAE mL -1 extract). HPLC analysis showed the presence of catechin (0.925 μg mL -1 ), rutin (0.831 μg mL -1 ), resveratrol (0.664 μg mL -1 ) and quercetin (0.456 μg mL -1 ) in the leaf extract, and catechin (0.127 μg mL -1 ), epicatechin (0.239 μg mL -1 ) and rutin (0.231 μg mL -1 ) in the seed extract. The results showed that the studied extracts exhibited a selective antimicrobial effect directed against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli) bacterial strains. The combination leaf extract/antibiotic had the highest synergistic effect when compared to combinations with seed and sheath extracts. The same extract with penicillin G, kanamycin and rifampin had highest synergetic effect against methicillinresistant S. aureus strain (MRSA), a strain that has gained great interest of microbiologists within the past decades. The chemical characterization of ethanol extracts from the vegetative and reproductive organs of R. pseudoacacia, the synergistic effects of certain antibiotics and acacia extracts and the potential to increase the antimicrobial activity of some commercial antibiotics against MRSA were investigated for the first time.

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“…One of the mechanisms of action proposed for lectins with antibacterial effect included the ability in forming pores in the cell membrane causing leakage of cellular content (Talas-Ogras et al 2005). In this sense, we evaluated whether the incubation of bacteria with WSMoL would result in the leakage of intracellular proteins.…”

Section: Discussionmentioning

confidence: 99%

Water-solubleMoringa oleiferalectin interferes with growth, survival and cell permeability of corrosive and pathogenic bacteria

et al. 2015

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Antibacterial activity of seed proteins of Robinia pseudoacacia

Cited by 22 publications

References 18 publications

Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity

Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity

Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia

Water-solubleMoringa oleiferalectin interferes with growth, survival and cell permeability of corrosive and pathogenic bacteria

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