Antibacterial activity of selected medicinal plants of northwest Pakistan traditionally used against mastitis in livestock

Abstract

The present study aimed to investigate the efficacy of traditionally used anti-mastitis plants ( and ) in northwest Pakistan against bacterial pathogens. Selected plants were phytochemically screened for Alkaloids, Flavonoids, and Saponins and checked for antibacterial activity at concentration of 50 mg/ml against and by agar well diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration was determined against multidrug resistant bacteria using tube dilution method. All extracts were found to significantly inhibit (p < 0.01, p < 0.05) the activity against bacterial strains examined. Among phytochemicals, alkaloids of all tested antimastitis plants produced significantly higher inhibition zones against bacteria. The minimum inhibitory concentration and minimum bactericidal concentration of phytochemicals and crude methanolic extracts against tested bacterial strains ranged between 12.5-50 mg/ml and 25-50 mg/ml, respectively. Medicinal plants traditionally used against mastitis are therapeutically active against bacterial pathogens. and were found to be potential candidate species for the development of novel veterinary drugs with low cost and fewer side effects.

Ethnomedicinal Plants in Herbal Remedies Used for Treatment of Skin Diseases by Traditional Healers in Songkhla Province, Thailand

“…Anthocyanin from Oryza sativa L. exhibited anti-inflammatory properties and anti-aging activity by modulating type I collagen gene expression and suppressing H 2 O 2 -induced NF-κB activation in skin fibroblasts [ 23 ]. The crude extract, alkaloids, flavonoids, and saponins from Oryza sativa L. showed antibacterial effects against multidrug resistant Staphylococcus aureus [ 24 ]. In addition, the antimicrobial activity of Oryza sativa L. against fungi [ 25 ] and viruses [ 26 ] has been revealed.…”

Prevalence of antibiotic resistant mastitis pathogens in dairy cows in Egypt and potential biological control agents produced from plant endophytic actinobacteria

“…for instance with traditional medicinal plants (Bullitta et al, 2018, Mushtaq et al, 2018). Recently, an in vitro study observed that the extracts derived from two medicinal plants, namely Allium sativum and Bunium persicum , were efficient against mastitis pathogens (Amber et al, 2018) .…”

Biologically active compounds of plant origin in medicine

“…B. Fedtsch., Oryza sativa L., and Triticum aestivum L. against S. aureus, E. coli, and Klebsiella pneumoniae was reported by Amber et al [2018]. High content of polyphenols with strong antibacterial activity against pathogens isolated from milk of mastitis-affected cows was detected in alcoholic extracts of Artemisia absinthium L. [Pașca et al 2017].…”