Antibacterial Activity of Silver-Hydroxyapatite/Titania Nanoparticles on Oral Bacteria

Abstract

Since bacterial accumulation surrounding biomaterials has pathogens known to cause the biomaterials centered infection, it may be important to develop some kind of biomaterial with antibacterial activity as well as biocompatibility. This study focused on evaluating the antibacterial activity of silver-hydroxyapatite/ Titania nanoparticles (Ag-nHA/nTiO2) against oral bacteria with agar dilution method. Bacteria were seeded on agar plate containing antibacterial material with different concentrations after incubation of 48 hours. The antibacterial activity was demonstrated by MICs. The MICs of Ag-nHA/nTiO2 ranged between 1000μg/ ml and 7500μg /ml under anaerobic conditions. And it also exhibited remarkable antibacterial activity to all the aerobe bacteria (MIC≤500μg/ml). On the other hand, the antibacterial activities of Ag-nHA/nTiO2 differentiate to some extent with the bacterial strains. This Ag-nHA/nTiO2 exhibited remarkable antibacterial activity to anaerobic and aerobe bacteria. This antibacterial effect may reduce the potential for bacterial colonisation of oral biomaterials with Ag-nHA/nTiO2.

Comparison of Antimicrobial Properties of Silver Nanoparticles Synthesized from Selected Bacteria

“…Further, the negatively charged Gram positive bacterial cell wall bind with more Ag ions which results in less number of AgNPs interacting with the plasma membrane thereby protecting the plasma membrane from the reactive oxygen species generated by the AgNPs leading to increased resistance observed. [43]. Further silver NPs mediate binding with DNA, protein denaturation, and respiratory functions leading to cell death [1,9,38].…”

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

“…14,15 A previous study has proved that the Ag + provided inhibitive effects on oral bacteria, such as Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum. 16,17 Therefore, the incorporation of silver ions or silver compounds in sealers may be advantageous.…”

Bacteria mediated silver nanoparticles: comparison as potent antibiofilm agents

“…The attachment of AgNPs to the surface of microbial cells was observed in this study using SEM and has also been reported by several groups. 17,19 Oza et al 20 reported that the antibacterial effect of AgNPs may be due to penetration of released Ag ions in bcterial cell wall. AgNPs can disrupt the cell wall and rapidly detach the biofilms.…”