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It is shown that the highest antimicrobial activity (100%) against strains ofKl. pneumoniaandSt. pneumoniaof strains causing pneumonia occurs when using protein-like fractions (AMP) obtained from CL after cultivation of LAB of strains of the genus Enterococcus isolated from fermented milk of goats and donkeys, and the lowest activity of AMP from CL of strains isolated from fermented milk of cows (20–50%). It was shown that the isolated polysaccharides of strains of the genus Enterococcus, which have antimicrobial activity, consist of glucose and galactose molecules. The difference in the effectiveness of the influence of polysaccharides depends on the source of the isolated pathogens, depends on the concentration of the polysaccharide, the genus and species of the strain causing pneumonia. It is concluded that some metabiotics of the Enterococcus genus are promising for use as bioinhibitors of the growth of pathogenic bacteria.
It is shown that the highest antimicrobial activity (100%) against strains ofKl. pneumoniaandSt. pneumoniaof strains causing pneumonia occurs when using protein-like fractions (AMP) obtained from CL after cultivation of LAB of strains of the genus Enterococcus isolated from fermented milk of goats and donkeys, and the lowest activity of AMP from CL of strains isolated from fermented milk of cows (20–50%). It was shown that the isolated polysaccharides of strains of the genus Enterococcus, which have antimicrobial activity, consist of glucose and galactose molecules. The difference in the effectiveness of the influence of polysaccharides depends on the source of the isolated pathogens, depends on the concentration of the polysaccharide, the genus and species of the strain causing pneumonia. It is concluded that some metabiotics of the Enterococcus genus are promising for use as bioinhibitors of the growth of pathogenic bacteria.
The comparative biodiversity of lactic acid bacteria (LAB) isolated from fermented dairy products produced in the southern regions of Armenia made from the milk of some domestic farm animals has been shown. Strains with probiotic properties belonging to Lactobacillus and Enterococcus genera were selected. LAB isolated from dairy products from different regions of Artsakh were mainly represented by strains of the genus Enterococcus. It has been shown that some strains of LAB during the growth process are able to synthesizing simultaneously different metabiotics – bacteriocins, polysaccharides, arginine, have bile salt hydrolase (BSH) activity which leads to the breakdown of bile salts and the taurine and glycine amino acids are formed as a result. The selected strains can be used for the production of functional foods. Ցույց է տրվել կաթնաթթվային բակտերիաների (ԿԹԲ) համեմատական կենսաբազմազանությունը, որոնք մեկուսացված են Հայաստանի հարավային շրջանների որոշ ընտանի գյուղատնտեսական կենդանիների կաթից արտադրված թթու կաթնամթերքից։ Ընտրվել են Lactobacillus և Enterococcus ցեղերին պատկանող պրոբիոտիկ հատկություններով օժտված շտամները: Արցախի տարբեր շրջանների թթու կաթնամթերքից մեկուսացված կաթնաթթվային բակտերիաները հիմնականում ներկայացված են Enterococcus ցեղի շտամներով: Ցույց է տրվել, որ ԿԹԲ-ների որոշ շտամներ աճման ընթացքում միաժամանակ սինթեզում են տարբեր մետաբիոտիկներ՝ բակտերիոցիններ, բազմաշաքարներ, արգինին, դրսևորում են լեղու աղերի հիդրոլազի (BSH) ակտիվություն, ինչը բերում է լեղու աղերի ճեղքմանը, և արդյունքում առաջանում են տաուրին և գլիցին ամինաթթուները: Ընտրված շտամները կարող են օգտագործվել ֆունկցիոնալ մթերքների արտադրության համար: Показано сравнительное биоразнообразие молочнокислых бактерий (МКБ), выделенных из кисломолочных продуктов, произведенных в южных регионах Армении из молока некоторых домашних сельскохозяйственных животных. Отобраны штаммы с пробио-тическими свойствами родов Lactobacillus и Enterococcus. МКБ, выделенные из кисломолочных продуктов из разных регионов Арцаха, в основном были представлены штаммами рода Enterococcus. Показано, что некоторые штаммы МКБ в процессе роста синте-зируют одновременно разные метабиотики – бактериоцины, полисахариды, аргинин; обладают активностью гидролазы желчных солей (BSH), что приводит к расщеплению солей желчных кислот с образованием аминокислот таурина и глицина. Отобранные штаммы могут быть использованы для производства продуктов функционального питания.
Richal is a traditional fermented dairy beverage that has a long history in the Iranian encyclopedia. This beverage contains whole milk, natural starter, herbs and salt. It can be produced in three different forms, Shiri (from milk), Masti (from yogurt), and Dooghi (from drinking yogurt). The present study was an investigation into the characteristic features of the lactic acid bacteria isolated from Richal Shiri. After preparing the traditional fermented beverage was investigated and several tests such as determining lactic acid and pH were performed on it. Next, lactic acid bacteria and yeasts were isolated from the traditional fermented beverage sample and cultured in different media such as fermented whole or skim milk, at different times temperatures and test cultures. Some strains had anti-bacterial activity against sporogenous microflora S. typhimurium G-38, B. subtilis 17-89, В. Thuringensis 69-6. Eleven Lactic acid bacteria strains were isolated; ten of them were Gram-positive, cocci, and one of them was Gram-positive, Bacillus. Ten strains were catalase-negative and one was catalase positive; the lactic acid bacteria had antibacterial activity by the determination which measuring zone diameters of bacterial growth inhibition. The functions of strains were different but the differences were not significant (p≥0.3). Between the 3 groups, however, B. subtilis 17-89 had the best function. According to the results, Richal Shiri has some lactic acid bacteria strains with anti-bacterial activity and the ability to ferment milk as a starter culture. The isolated fermented milk, according to its properties, may introduce a new functional food product for development in the food industry. These bacteria will be used in future investigations.
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