Antibacterial Activity of the Extracts Obtained from Rosmarinus officinalis, Origanum majorana, and Trigonella foenum-graecum on Highly Drug-Resistant Gram Negative Bacilli

Abdel-Massih, Roula M.; Abdou, Elias; Baydoun, Elias; Daoud, Ziad

doi:10.1155/2010/464087

Cited by 24 publications

(11 citation statements)

References 12 publications

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“…The antimicrobial potential of plant species has been explored mostly via studies of their essential oils (EOs) [12,13,[22][23][24][25][26][27][28][29][30][31][32][33][34]. Due to the toxic effect exhibited by EOs, it is of utmost importance to explore other plant-derived therapeutic alternatives.…”

Section: Discussionmentioning

confidence: 99%

Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity, Mode of Action and Phenolic Characterization

et al. 2020

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Medicinal and aromatic plants are known to have a wide range of uses and health benefits, and should be exploited for their bioactivity. Here we evaluated the antimicrobial activity of decoctions of Satureja montana L. and Origanum majorana L. against Gram-positive and Gram-negative bacteria and Candida spp. as well as their mechanism of action and phenolic characterization. The Satureja montana and Origanum majorana extracts were effective against a broad set of species, including the Gram-positive Staphylococcus aureus, Enterococcus faecalis, and Streptococcus dysgalactiae and the Gram-negative Klebsiella pneumoniae and Pseudomonas aeruginosa. Both extracts were found to have rosmarinic acid as the main phenolic compound and to exert their antimicrobial activity at the level of the cell membrane. Membrane perturbations by the extracts impaired cell membrane integrity only a few hours after exposure. This study confirms the bioactive potential of Satureja montana and Origanum majorana decoctions, and supports the development of novel formulations with wide antimicrobial properties based on these medicinal and aromatic herbs.

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Section: Discussionmentioning

confidence: 99%

Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity, Mode of Action and Phenolic Characterization

et al. 2020

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“…In our study, Origanum majorana was highly inhibitory to the growth of Staphylococcus aureus, recording the lowest MIC in this study with the dichloromethane, ethyl acetate and aqueous extracts which were all 0.625μg/μl (Table 3). Similarly, Abdel-Massih et al 38 showed that the ethyl acetate fraction of Origanum majorana had the best antimicrobial effect on E. coli and K. pneumoniae exhibiting the lowest MIC 90 (1.28 μg/μl). Leeja et al 28 also studied the antimicrobial activity of Origanum majorana against Staphylococcus aureus using the disc diffusion method.…”

Section: Antimicrobial Activity Of Rheum Rhaponticummentioning

confidence: 92%

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2014

IJPSR

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Background: Bacterial resistance to antimicrobial agents is on the rise and this is causing serious complications resulting in increased morbidity and mortality of bacterial infections. There is a need for new antimicrobial molecules in order to fight against Multi-Drug-Resistant Organisms. The Mediterranean area is rich in a variety of medicinal plants and this may represent a potential for new compounds and molecules with enhanced antibacterial activity. Methods: The antimicrobial effects of three traditionally used Lebanese plants were investigated against 24 clinical isolates of Staphylococcus aureus with different phenotypes of resistance. Rosmarinus officinalis, Rheum rhaponticum, and Origanum majorana where extracted with ethanol, then further subfractionated with petroleum ether, dichloromethane and ethyl acetate. The remaining aqueous fraction was also collected, thus a total of five extracts were studied for each plant. The MIC and MBC of these extracts were determined using the microdilution technique. Results: Rosmarinus officinalis was the most effective against most of the strains studied including MRSA, QS, QR, MS and MR. The ethyl acetate fraction of Rosmarinus officinalis, Rheum rhaponticum, and Origanum majorana showed significant antibacterial activity against S. aureus with MIC ranging between 2.5 and 5µg/µl. The crude extract of Rheum rhaponticum was also highly effective at a low concentration of 4.25µg/µl. Conclusions: Most extracts showed antimicrobial activity at low concentrations. Antimicrobial activity of the plant extracts varied with the profile of resistance of the bacterial isolate. Further studies need to investigate the active compounds in these extracts and their mode of action to make use of them as antibiotics and food preservatives. INTRODUCTION: The Middle East area has witnessed a surge in methicillin-resistant Staphylococcus aureus over the past two decades 1 .

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“…Several additional studies that stretch beyond the scope of single-center studies are needed as well in order to shed light on the prevalence of resistance across the country. Nevertheless, few novel studies targeting endogenous plants are being conducted in Lebanon in an attempt to discover new molecules that exert antibiotic activity [54,55]. Whether these attempts would be successful and helpful in the ongoing effort to combat resistant strains remains to be unveiled.…”

Section: Antimicrobial Consumption and Conclusionmentioning

confidence: 99%

Resistance of Gram-Negative Bacilli in Lebanon

Salem

Daoud

2013

ISRN Infectious Diseases

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Several studies have reported the isolation of resistant Gram-Negative Bacilli in Lebanon. However, those studies are new and scarce as compared to worldwide data and mostly restricted to single center studies. In this review, we attempt to provide a reliable and comprehensive report describing the current situation and providing prospects for bacterial resistance in Lebanon. Several studies have shown that Extended Spectrum -Lactamase-producing E. coli and K. pneumoniae strains are being increasingly reported. Moreover, 2.15% of E. coli and 7.84% of K. pneumoniae isolates have shown carbapenem resistance and up to 30% of isolated E. coli strains were found to be Multi-Drug Resistant. Molecular studies showed that the most widespread -Lactamases in Lebanon are of the CTX-M-15 and SHV types. In addition, K. pneumoniae strains producing metallo--Lactamase and Klebsiella pneumoniae Carbapenemase have been reported. Resistant Pseudomonas aeruginosa and Acinetobacter baumannii caused several nosocomial infections and some Acinetobacter baumannii strains were found to produce OXA-58 type ESBL. e few data addressing the rate of antibiotic consumption in Lebanon show a high rate of antibiotic misuse and abuse. In conclusion, there is a need for antibiotic stewardship programs and additional studies that go beyond the scope of single-center studies in Lebanon.

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Antibacterial Activity of the Extracts Obtained from Rosmarinus officinalis, Origanum majorana, and Trigonella foenum-graecum on Highly Drug-Resistant Gram Negative Bacilli

Cited by 24 publications

References 12 publications

Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity, Mode of Action and Phenolic Characterization

Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity, Mode of Action and Phenolic Characterization

Untitled

Resistance of Gram-Negative Bacilli in Lebanon

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