Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes

Abstract

Algeria honey, in-vitro, possess antibacterial activity.

In vitro anti-biofilm and anti-bacterial activity of Junceella juncea for its biomedical application

“…The maximum zone of inhibition was observed in S. typhii and minimum inhibition was recorded in S. flexneri. Previously, several of the report on anti-microbial activity of gorgonian extract have been studied and published [21][22][23][24][25][26][27][28][29][30][31][32] . Sastry et al [22] have reported ethyl acetate extract of J. juncea showed enhanced antibacterial activity against B. subtilis, B. pumilis and E. coli.…”

“…Congo red dye (0.8 g/L) was also prepared simultaneously and added to warm (55 °C ) BHI agar. The media was poured on to Petri dishes, inoculated and incubated for [24][25][26][27][28][29][30][31][32][33][34][35][36] h at 37 °C .…”

“…Individual wells were filled with 180 µL BHI broth followed by inoculation with 10 µL of overnight pathogenic bacterial culture. To this 10 µL MGE was added from the prepared stock solution of 10,20,30,40,50,60,70,80,90 and 100 µg/mL respectively along with control (without test sample) and incubated at 37 °C for 24 h. After incubation, content in the wells were removed, washed with 0.2 mL of phosphate buffer saline (PBS) pH 7.2 to remove free floating bacteria. The adherence of sessile bacteria were fixed with sodium acetate (2%) and stained with crystal violet (0.1%, w/v).…”

Evaluation of the Antimicrobial Activity of Certain Irradiated and Non- Irradiated Egyptian Honey Bees' Products

“…The spread plate count technique was used (26) , 1ml of each honey sample was mixed with 1ml of the sterile tween-saline solution (0.9% w/v NaCl in distilled water containing 0.1% v/v tween 80, adjusted at pH 7) on a vortex (type paramix II No. 65, West Germany) for 3min, and 2-fold serial dilutions were made in the same diluent.…”

“…According to (26) , the antibacterial activity was classified as: not sensitive (<8 mm); sensitive (8 to 14mm); very sensitive (15 to 19mm) and extremely sensitive (>20mm). Table ( 4) shows that the antimicrobial activity of the honey samples against the pathogenic microorganisms decreased upon dilution.…”