Antibacterial activity of violacein against Staphylococcus aureus isolated from Bovine Mastitis

Cazoto, Luciana Lacorte; Martins, Dorival; Ribeiro, Márcio Garcia; Durán, Nélson; Nakazato, Gerson

doi:10.1038/ja.2011.13

Cited by 62 publications

(60 citation statements)

References 11 publications

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“…Since it is well known that violacein has a wide range of biocidal effects [6], [81]–[86], we were interested to test if HH01 affects survival and development of C. elegans . In addition we wanted to elucidate, if possibly other factors or compounds produced by HH01 would influence C. elegans survival and development.…”

Section: Resultsmentioning

confidence: 99%

The Janthinobacterium sp. HH01 Genome Encodes a Homologue of the V. cholerae CqsA and L. pneumophila LqsA Autoinducer Synthases

et al. 2013

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Janthinobacteria commonly form biofilms on eukaryotic hosts and are known to synthesize antibacterial and antifungal compounds. Janthinobacterium sp. HH01 was recently isolated from an aquatic environment and its genome sequence was established. The genome consists of a single chromosome and reveals a size of 7.10 Mb, being the largest janthinobacterial genome so far known. Approximately 80% of the 5,980 coding sequences (CDSs) present in the HH01 genome could be assigned putative functions. The genome encodes a wealth of secretory functions and several large clusters for polyketide biosynthesis. HH01 also encodes a remarkable number of proteins involved in resistance to drugs or heavy metals. Interestingly, the genome of HH01 apparently lacks the N-acylhomoserine lactone (AHL)-dependent signaling system and the AI-2-dependent quorum sensing regulatory circuit. Instead it encodes a homologue of the Legionella- and Vibrio-like autoinducer (lqsA/cqsA) synthase gene which we designated jqsA. The jqsA gene is linked to a cognate sensor kinase (jqsS) which is flanked by the response regulator jqsR. Here we show that a jqsA deletion has strong impact on the violacein biosynthesis in Janthinobacterium sp. HH01 and that a jqsA deletion mutant can be functionally complemented with the V. cholerae cqsA and the L. pneumophila lqsA genes.

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Section: Resultsmentioning

confidence: 99%

The Janthinobacterium sp. HH01 Genome Encodes a Homologue of the V. cholerae CqsA and L. pneumophila LqsA Autoinducer Synthases

et al. 2013

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“…1), is a purple pigment produced by some bacteria and displays various important biological activities, including antibacterial (Cazoto et al 2011;Nakamura and Sawada 2003), antifungal (Becker et al 2009), antiviral (Andrighetti-Frohner et al 2003), apoptosis-inducing properties in cancer cells (Bromberg et al 2005;Ferreira et al 2004), antioxidant (Konzen et al 2006), trypanocidal (Durán et al 1994), antidiarrheal (Antonisamy et al 2009), analgesic and antipyretic (Antonisamy and Ignacimuthu 2010), and antimalarial effects (Duran et al 2007). Recently, it has been reported that intraperitoneal doses of Vio up to 1 mg kg −1 are not toxic in mouse blood, kidneys, or liver, resolving the main issues regarding Vio cytotoxicity to several tumor models in vivo and providing good reference material for the use in vivo of Vio and its derivatives as a therapeutic compound with few side effects (Cazoto et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment

Jiang

Wang

Fang

et al. 2012

Appl Microbiol Biotechnol

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Violacein (Vio) is an important purple pigment with many potential bioactivities. Deoxyviolacein, a structural analog of Vio, is always synthesized in low concentrations with Vio in wild-type bacteria. Due to deoxyviolacein's low production and difficulties in isolation and purification, little has been learned regarding its function and potential applications. This study was the first effort in developing a stable and efficient biosynthetic system for producing pure deoxyviolacein. A recombinant plasmid with vioabce genes was constructed by splicing using an overlapping extension-polymerase chain reaction, based on the Vio-synthesizing gene cluster of vioabcde, originating from Duganella sp. B2, and was introduced into Citrobacter freundii. With the viod gene disrupted in the Vio synthetic pathway, Vio production was completely abolished and the recombinant C. freundii synthesized only deoxyviolacein. Interestingly, vioe gene expression was strongly stimulated in the viod-deleted recombinant strain, indicating that viod disruptions could potentially induce polar effects upon the downstream vioe gene within this small operon. Deoxyviolacein production by this strain reached 1.9 g/L in shaker flasks. The product exhibited significant acid/alkali and UV resistance as well as significant inhibition of hepatocellular carcinoma cell proliferation at low concentrations of 0.1-1 μM. These physical characteristics and antitumor activities of deoxyviolacein contribute to illuminating its potential applications.

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“…Moreover, this purple derivative has synergistic effects when combined with other antibiotics, especially penicillin, against resistant strains, including those multidrug-resistant, with MIC values in the range of 0.04 μmol·dm −3 . In contrast, no activity was detected against E. coli (Cazoto et al, 2011). Thus, it is reasonable to propose violacein as a possible antibacterial agent to be used for the treatment of bovine mastitis, either alone or in combination with other antibiotics.…”

Section: Antimicrobial Potentialmentioning

confidence: 99%

“…The ability of violacein to kill Staphylococcus aureus (S. aureus) and E. coli field strains isolated from bovine mastitis was investigated (Cazoto et al, 2011). These are the most frequent etiological agents of bovine mastitis, where they are responsible for serious economic losses in milk production, estimated in billions of dollars per year in the United States alone (Unnerstad et al, 2009).…”

Section: Antimicrobial Potentialmentioning

confidence: 99%

Advances in Chromobacterium violaceum and properties of violacein-Its main secondary metabolite: A review

Justo

Durán

Brocchi

et al. 2016

Biotechnology Advances

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110

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Antibacterial activity of violacein against Staphylococcus aureus isolated from Bovine Mastitis

Cited by 62 publications

References 11 publications

The Janthinobacterium sp. HH01 Genome Encodes a Homologue of the V. cholerae CqsA and L. pneumophila LqsA Autoinducer Synthases

The Janthinobacterium sp. HH01 Genome Encodes a Homologue of the V. cholerae CqsA and L. pneumophila LqsA Autoinducer Synthases

Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment

Advances in Chromobacterium violaceum and properties of violacein-Its main secondary metabolite: A review

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