Antibacterial activity of water extracts and essential oils of various aromatic plants against Paenibacillus larvae, the causative agent of American Foulbrood

González, María J.; Marioli, Juan M.

doi:10.1016/j.jip.2010.04.005

Cited by 74 publications

(58 citation statements)

References 29 publications

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“…[31] In this study, P. larvae showed significant growth decline when treated with both plant extracts. These findings were comparable to the effects of two aqueous extracts of Eucalyptus cinerea and Minthostachys verticillata, which showed 100% efficacy against P. larvae strains [32]; therefore, the present research was further supported by previous studies. [33,34] The present study showed that the applications of botanical extracts were efficacious against bee gut bacteria and Varroa and, thus, were recommended for further control measures of mite and American foulbrood (AFB) disease of honeybee.…”

Section: Resultssupporting

confidence: 89%

Controlling honeybee pathogen by using neem and Barbaka plant extracts

Anjum

Ayaz

Shah

et al. 2015

Biotechnology & Biotechnological Equipment

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The honeybees (Apis mellifera) population is declining. The involved causes may be pathogens (mites, viruses and bacteria) and parasites, due to honeybee's compromised immune system, leading to various bee-associated infections. Therefore, the present study assessed the comparative efficacy of plant extracts, including neem (Azadirachta indica) and Barbaka (Vitex trifolia) against gut bacteria and ectoparasitic mite Varroa destructor of honeybee A. mellifera (Hymenoptera: Apidae). The in vitro activities of the plant extracts were determined by using standard methods against five bee gut bacterial isolates, including the well-known bee pathogenic bacteria Paenibacillus larvae. Miticides were also assessed in field against honeybee mites. The obtained results from the phytochemical screening of Barbaka and neem extracts efficiency showed inhibitory zones with diameters of 23 mm with 60 mg/mL against P. larvae and 14 mm with 60 mg/mL against Escherichia coli, respectively. None of the extracts proved to be effective against Salmonella enterica and the neem extract showed intermediate activity against Bacillus subtilis and Staphylococcus hominis. Likewise, Barbaka plant extracts were not effective against B. subtilis. Similarly, the relative treatment efficacies of neem and Barbaka extracts, together with conventional miticides against honeybee Varroa mites, varied significantly. However, the effect of Barbaka and neem extracts on the mite-infested colonies was lower than the effect of other treatments, but it was also higher than in the control colonies. This study concluded that Barbaka and neem extracts have antibacterial and miticidal activity and are reasonably safe. However, more trials have to be conducted, in order to validate these results.

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Section: Resultssupporting

confidence: 89%

Controlling honeybee pathogen by using neem and Barbaka plant extracts

Anjum

Ayaz

Shah

et al. 2015

Biotechnology & Biotechnological Equipment

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“…In literature are reported many papers on the activity of plants, essential oils and methanol or ethanol extracts to retard or inhibit the growth of bacteria, yeast and moulds [1][2][3]. Nowadays, the increasing resistance of microorganism to some antibiotics makes it necessary to continue the search of new antimicrobial active substances from plants.…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of <i>Spartium junceum</i> L. (Fabaceae) Aromatic Water

Cerchiara¹,

Blaiotta²,

Straface³

et al. 2013

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In this study, antimicrobial, antioxidant and cytotoxic activities of Spartium junceum L. aromatic water (SJAW), obtained by vacuum process distillation, were investigated. Antimicrobial activity of SJAW was evaluated against fungal organisms, Gram-positive and Gram-negative bacteria using agar-well diffusion method. Moreover, the ability of SJAW to sensitize Staphyloccus aureus to antibiotics was also evaluated. The antioxidant activity was examined by 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and the cytotoxic activity of SJAW main components was tested on melanoma RPMI 7932 and normal keratinocytes NCTC 2544 cell lines using the trypan blue method. The preparation did not show antibacterial activity against tested microorganisms and was unable, at tested concentrations/conditions, to sensitize Staphylococcus aureus to antibiotics clindamycin (2 μg) and tetracycline (30 μg). On the contrary SJAW possesses an appreciable antioxidant and cytotoxic activity suggesting its potential use for pharmaceutical applications.

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“…All the EOs presented in this work, except for the EO of S. chilensis, have been previously studied as P. larvae antimicrobials Ruffinengo et al, 2006;Fuselli et al, 2008;González & Marioli, 2010). The antimicrobial control strategy is most commonly used to eliminate a pathogen from a host, however, the use of EOs as antipathogenic, i.e.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory action of essential oils against proteases activity of Paenibacillus larvae, the etiological agent of American Foulbrood disease

Pellegrini

Zalazar

Fuselli

et al. 2018

Span. j. agric. res.

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American foulbrood (AFB) is a disease affecting the larva of Apis mellifera. The etiological agent is Paenibacillus larvae, which releases metalloproteases involved in the degradation of larval tissues. Through quorum sensing (QS) mechanism, bacteria are able to activate specific genes such as virulence factors. The exoproteases regulation of P. larvae could be associated with QS. A promising mechanism of AFB control is to block QS mechanism with essential oils (EO). The aim of this study was to investigate the potential presence of QS signals in the regulation of P. larvae proteases and the effect of seven EOs on the exoproteases activity of P. larvae. From growth curves and evaluation of the presence of proteases by milk agar plates assay, it was observed protease activity during the late exponential phase of growth. Early production of protease activity (15 hours earlier than control) was observed when a low density culture was incubated with late exponential spent medium (SM) suggesting the presence of factor(s) inducing this activity. SM was obtained by the ultrafiltration of P. larvae cultures on late growth phase and was free of proteases. Proteolytic activity was quantified on P. larvae cultures in presence of sublethal concentration of EO by azocasein method. The EOs, except S. chilensis EO, reduced significantly protease activity (more than 50%). We report for the first time evidence on the possible role of QS on P. larvae and the antiproteolytic activity of EOs (except for S. chilensis) on exoproteases, an interesting therapeutic strategy to control AFB.

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Antibacterial activity of water extracts and essential oils of various aromatic plants against Paenibacillus larvae, the causative agent of American Foulbrood

Cited by 74 publications

References 29 publications

Controlling honeybee pathogen by using neem and Barbaka plant extracts

Controlling honeybee pathogen by using neem and Barbaka plant extracts

Biological Activity of <i>Spartium junceum</i> L. (Fabaceae) Aromatic Water

Inhibitory action of essential oils against proteases activity of Paenibacillus larvae, the etiological agent of American Foulbrood disease

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Antibacterial activity of water extracts and essential oils of various aromatic plants against Paenibacillus larvae, the causative agent of American Foulbrood

Cited by 74 publications

References 29 publications

Controlling honeybee pathogen by using neem and Barbaka plant extracts

Controlling honeybee pathogen by using neem and Barbaka plant extracts

Biological Activity of &lt;i&gt;Spartium junceum&lt;/i&gt; L. (Fabaceae) Aromatic Water

Inhibitory action of essential oils against proteases activity of Paenibacillus larvae, the etiological agent of American Foulbrood disease

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Biological Activity of <i>Spartium junceum</i> L. (Fabaceae) Aromatic Water