Antibacterial activity of β-sitosterol isolated from the leaves of Odontonema strictum (Acanthaceae)

Luhata, Lokadi Pierre; Usuki, Toyonobu

doi:10.1016/j.bmcl.2021.128248

Cited by 26 publications

(12 citation statements)

References 29 publications

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“… 16 β-Sitosterol (6) is considered a potent antimicrobial component. 21 Thus, compound 6 might be the most active compound in the studied mycobiont. These findings suggest that compounds derived from D. pruinosum could serve as promising candidates for alternative or complementary antimicrobial agents, particularly considering their effectiveness against a multidrug-resistant bacterial strain.…”

Section: Resultsmentioning

confidence: 97%

Antimicrobial sesquiterpenes from the cultured mycobiont Diorygma pruinosum against methicillin-resistant Staphylococcus aureus isolated from Vietnamese street foods

Le,

Duong,

Nguyen

et al. 2024

RSC Adv.

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Section: Resultsmentioning

confidence: 97%

Antimicrobial sesquiterpenes from the cultured mycobiont Diorygma pruinosum against methicillin-resistant Staphylococcus aureus isolated from Vietnamese street foods

Le,

Duong,

Nguyen

et al. 2024

RSC Adv.

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“…The previous report also suggested that 3-hydroxy-isoagatholactone ( 2 ) was identified and tested for the first time and displayed low to moderate antibacterial activities, whereas, in the present work, the activities of the compound were also studied and better results were generated. Many works have been reported on the antimicrobial activities of β -sitosterol ( 1 ) and results revealed that the compound possesses low to moderate activities against different bacterial pathogens [ 31 , 44 , 45 ]. The previous activities were also in good agreement with the findings of the present study.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of Cyphostemma adenocaule: A Combined In Vitro and In Silico Study

Gebrehiwot,

Melaku,

Aliye

et al. 2024

Journal of Tropical Medicine

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Cyphostemma adenocaule is a therapeutic plant traditionally used to treat rabies, snake bite, diarrhea, and wound healing. To address the bioactive compounds exhibiting these activities, we performed a comprehensive study on the roots of the plant. Thus, the present study aims to inspect the in vitro antioxidant and antibacterial efficacies of compounds isolated from the combined dichloromethane : methanol (1 : 1) and methanol extracts of C. adenocaule along with the in silico study of their interaction with selected protein targets. The silica gel column chromatography technique was used for the isolation of compounds, and the antibacterial and antioxidant activities were evaluated using agar disc diffusion and DPPH radical scavenging assays, respectively. Furthermore, in silico molecular docking screening, pharmacokinetics, and toxicity protocols of the compound isolates were performed to offer the potential applications of the compounds in developing novel medications. A BIOVIA Discovery Studio in combination with AutoDock Vina 4.2 software, SwissADME, and ProTox-II prediction web tools were used to generate the molecular docking, pharmacokinetics, and toxicity profiles, respectively. Notably, the chromatographic separation of the combined extracts yielded six known compounds, namely, β-sitosterol (1), 3-hydroxyisoagatholactone (2), ε-viniferin (3), myricetin (4), tricuspidatol A (5), and parthenocissin A (6). The in vitro antibacterial activities revealed the highest inhibition zone by tricuspidatol A (5) (16.67 ± 0.47), showcasing its potent activity against S. aureus at 2 mg/mL, compared to ciprofloxacin (21.50 ± 0.41). ε-Viniferin (3) (IC50: 0.32 μg/mL) exhibited greater antioxidant activity than the others and displayed promising results compared to ascorbic acid (0.075 μg/mL). The molecular docking study revealed the highest binding affinity by ε-viniferin (3) (−9.9 kcal/mol) against topoisomerase II α. 3-Hydroxyisoagatholactone (2) and ε-viniferin (3) fulfilled Lipinski’s rule with no violation, and the organ toxicity predictions revealed that all the compounds showed no cytotoxicity and hepatotoxicity effects. Thus, this study’s combined in vitro and in silico outcomes suggest the potential use of the isolated compounds in drug discovery and support the traditional relevance of C. adenocaule.

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“…Several of these compounds are potentially bioactive. β-Sitosterol, a phytosterol present naturally in the cells and membranes of plants, has been shown to have antibacterial and antioxidant activities with various applications; ,− cholest-5-en-3-yl (9 Z )-9-octadecenoate was found in the extracts that showed anticancer and antibacterial activities; 1-dodecanol is a long-chain fatty acid with high potential as antibacterial (MIC, 8 μg/mL) and antimycobacterial agents; , neophytadiene is a diterpenoid compound that possesses antimicrobial, anti-inflammatory, antibiofilm, and antioxidant properties; , nonadecene, hexanol, and decane exhibit antibacterial activities. − Most of the major compounds were identified from ME of A . polystachyus (seven out of eight), which has known bioactive functions.…”

Section: Results and Discussionmentioning

confidence: 99%

“…albicans , indicating antifungal activity of ME. The compounds listed in Table from ME such as β-sitosterol, cholest-5-en-3-yl (9 Z )-9-octadecenoate, 1-dodecanol, neophytadiene, ( E )-2-nonadecene, hexanol-4-D2, and decane possess antimicrobial properties − that appear to be responsible for the observed inhibition of the bacterial and fungal species.…”

Section: Results and Discussionmentioning

confidence: 99%

Phytochemical Composition, Antioxidant, Antimicrobial, Antibiofilm, and Antiquorum Sensing Potential of Methanol Extract and Essential Oil from Acanthus polystachyus Delile (Acanthaceae)

Getahun,

Nesru,

Ahmed

et al. 2023

ACS Omega

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The evolution of microbes in response to conventional antimicrobials leads to antimicrobial resistance (AMR) and multidrug resistance (MDR), and it is a global threat to public health. Natural products are possible solutions to this massive challenge. In this study, the potential of Acanthus polystachyus extracts was investigated for phytochemical composition and biological properties as antimicrobials. Gas chromatography-mass spectra (GC-MS) analysis of methanol extract (ME) and essential oil (EO) detected 79 and 20 compounds, respectively. The major compounds identified in ME and their abundance were β-sitosterol acetate (16.06%), cholest-5-en-3-yl (9Z)-9-octadecenoate (9.54%), 1-dodecanol (7.57%), (S)-(E)-(−)-4-acetoxy-1-phenyl-2-dodecen-1-one (6.03%), neophytadiene (5.7%), (E)-2-nonadecene (3.9%), hexanol-4-D2 (2.92%), and decane (2.4%). Most compounds have known bioactive functions. In EO, the major compounds were stearyl alcohol (25.38%); cis-9-tetradecenoic acid, isobutyl ester (22.95%); butyl 9-tetradecenoate (10.62%); 11,13-dimethyl-12-tetradecen-1-ol acetate (10.14%); ginsenol (3.48%); and diisooctyl phthalate (2.54%). All compounds are known to be bioactive. The antioxidant activity of ME and EO ranged from 48.3 to 84.2% radical scavenging activity (RSA) and 45.6 to 82% RSA, respectively, with dose dependency. The disc diffusion assay for the antimicrobial activity of ME revealed high inhibition against Acenetobacter baumannii (130.2%), Pseudomonas aeruginosa (100.3%), and Staphylococcus aureus (87.7%). The MIC, MBC/MFC, and MBIC values for ME were 0.5–1.0, 2–4, and 0.5–1.0 mg/mL and for EO were 0.31–0.62, 1.25–2.5, and 0.31–0.62 μL/mL, respectively, indicating inhibition potential as well as inhibition of biofilm formation. The tolerance test values indicated bactericidal activity against most strains and bacteriostatic/fungistatic activity against A. baumannii, E. faecalis, and C. albicans. The antiquorum sensing activity of ME achieved by pyocyanin inhibition assay on P. aeruginosa showed a 51.6% inhibition at 500 μg/mL. These results suggest that ME and EO derived from A. polystachyus leaves are potent, valuable, cost-effective antioxidants and antimicrobials. Both extracts may effectively combat pathogenic and resistant microbes.

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Antibacterial activity of β-sitosterol isolated from the leaves of Odontonema strictum (Acanthaceae)

Cited by 26 publications

References 29 publications

Antimicrobial sesquiterpenes from the cultured mycobiont Diorygma pruinosum against methicillin-resistant Staphylococcus aureus isolated from Vietnamese street foods

Antimicrobial sesquiterpenes from the cultured mycobiont Diorygma pruinosum against methicillin-resistant Staphylococcus aureus isolated from Vietnamese street foods

Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of Cyphostemma adenocaule: A Combined In Vitro and In Silico Study

Phytochemical Composition, Antioxidant, Antimicrobial, Antibiofilm, and Antiquorum Sensing Potential of Methanol Extract and Essential Oil from Acanthus polystachyus Delile (Acanthaceae)

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