Antibacterial Activity Test Turmeric(Curcuma longa L.) Extract Herbal Oil in Extra Virgin Olive Oil Against Staphylococcus aureus and Propionibacterium acnes

Abstract

Turmeric and olive oil can be combined to produce herbal oil extracts. The purpose of this study was to determine the content of active compounds and to determine the effect of variations in the concentration of extracts on antibacterial activity. Turmeric is combined with extra virgin olive oil using the hot maceration method. The combination is formulated into five turmeric concentration namely 10%, 20%, 30%, and 40% in 100 mL of solution with variations in oven temperature of 30 °C, 40 °C, 50 °C, and 60 °C. The phytochemical test of secondary metabolite compounds, triterpenoid, flavonoid, and tannin. The herbal oil extract positive for triterpenoid and flavonoid compounds. The results of the antibacterial test against Staphylococcus aureus at concentrations of 10%, 20%, 30%, and 40% is 1.7 mm, 3.3 mm, 3.6 mm, and 3.9 mm, while in Propionibacterium acne is 1.6 mm, 2.6 mm, 2.9 mm, and 3.6 mm. Total Plate Count (TPC) test on the herbal oil extract against Staphylococcus aureu produces living microbes for 5,2x10 8 CFU/ mL, 4,8x10 8 CFU/mL, 3,7x10 8 CFU/mL and 3,4x10 8 CFU/mL, while in Propionibacterium acne for 3,0x10 8 CFU/ mL, 2,0x10 8 CFU/ mL, 1,6x10 8 CFU/ mL, and 1,3x10 8 CFU/ mL.